This research aims to contribute to our knowledge of the chronology of the main cultural entities of the Bronze Age in the Southern Urals. The objectives of this work include the verification of earlier conclusions regarding individual sites, expanding the date series for the key cultures with reliable dates, and creating reference series for the Bayesian modeling of key archaeological sites. Thirty-two samples were selected from reliable contexts. They reflect seven different cultural traditions of the 2nd millennium calBC (Sintashta, Srubnaya, Alakul, Fedorovka, Cherkaskul, Final Bronze Age1, Transition to Early Iron Age2). Collagen (human and domestic animal bones), charcoal, and wood samples were used for radiocarbon dating. Pairs of different-type samples (human bone – animal bone, animal bone – charcoal) were obtained for the same undisturbed burial and the building floor at the time of its abandonment. The data and the composition of δ15N and δ13C isotopes allow the new dates to be considered reliable. Furthermore, the new results do not conflict with the previously obtained accelerator mass spectrometry (AMS) dates. Determining boundary intervals for the main cultures of the Andronovo cultural community (Alakul and Fedorovka) resulted in statistically reliable conclusions on their relationship. The Alakul culture appeared earlier than the Fedorovka culture. The latter has a migratory origin in the Southern Urals. The two traditions have a long history of interaction, but the Alakul culture ending earlier. The series of dates for the Final Bronze Age, divided into two sub-periods, has been significantly expanded. Bayesian modeling of the chronology of the stratified settlement Sintashta II (Levoberezhnoe) made it possible to determine the intervals of the main phases of its history: 2004–1852 calBC (Sintashta culture) and 1410–1170 calBC (Final Bronze Age). Intermediate Cherkaskul phase is represented by a single date (1731–1541 cal BC).