The economic injury level (EIL) of the maize stem borer, Chilo partellus (Swinhoe) was determined by artificially infesting maize (cv. Katumani) plants at three different growth stages; viz, 20, 40 and 60 days old plants with varying densities of newly hatched larvae; viz, 2, 4, 6, 8, 10 and 12 per plant constituting the borer population. The grain yield data obtained from the infested and the protected plants showed that maximum yield reduction and stalk damage occurred to the 20-day old crop, while there was insignificant larval effect on yields for the 60-day old crop. Regression analyses gave a significant linear equation (y = a + bx) which estimated the yields per plant at different infestation levels and these were used to calculate the economic injury level at each stage. The resultant EIL was 3.2 and 3.9 larvae/plant for 20- and 40-day old plants, respectively.