This research investigates a novel method for cultivating mycelium-based leather substitutes using a carefully formulated paste consistency substrate. The primary objectives are to enhance nutrient availability, facilitate scalability, and streamline cultivation processes. The study spans a 21-day cultivation period, during which a flower-based medium is employed, eliminating the need for labor-intensive harvesting techniques. Two fungal species, Ganoderma lucidum (rishi) and Pleurotus djamor (pink oyster) are tested to assess their compatibility with the growth method. These species were chosen based on their rapid colonization rates and inherent resilience. The investigation delves into various combinations of crosslinking agents, including glycerol (a plasticizer), commercial tanner, citric acid, and magnesium sulfate. The effects of these agents on tensile strength are compared and qualitative data is analyzed through the use of scanning electron microscopy (SEM) and stereo microscopy. Furthermore, the study explores the fabrication potential of non-woven textiles derived from mycelium, emphasizing their suitability as eco-friendly leather alternatives. Scaled prototypes are highlighted to demonstrate their feasibility. Post-treatment processes, such as dyeing with bio-based dyes and acrylic leather paint, are evaluated for their aesthetic impact. The research contributes a biodegradable material alternative that addresses the environmental challenges of high textile consumption. The findings add to the growing body of sustainable design methods in the realm of leather-like materials in bio-design.