Field experiments from 1997 to 1999 examined cotton cv. ‘Coker 312’ that was genetically transformed to tolerate glufosinate. None of the glufosinate treatments caused visible injury to the glufosinate-tolerant cotton, but treatments were lethal to nontransformed or nonexpressing cotton. No glufosinate treatment adversely affected plant height at maturity, total number of nodes, bolls per plant, or boll positions. Glufosinate applications of 0.6 kg ha−1 made at eight stages of growth, ranging from cotyledon stage to 50% open boll, did not adversely affect yield or fiber quality as measured by micronaire or fiber length and strength. Sequential glufosinate applications up to four stages of growth from the zero- to one-leaf stage to the 14- to 15-leaf stage or individual glufosinate applications at 3.3 kg ha−1 made at the two- to three-leaf stage of growth also did not adversely affect yield or fiber quality. Overall yields in these studies were low relative to normal Texas Southern High Plains cotton yield because these studies were conducted using a Coker 312 parental line, which is generally a poor performer in this region. This research indicated that the transformation events for glufosinate tolerance in cotton were successful and the glufosinate-tolerance gene was expressed throughout the growing season. Transformation and field testing of other cotton varieties are needed to improve varietal performance on the Texas Southern High Plains.