Tree-ring cellulose is a commonly used material for radiocarbon analysis. Extracting cellulose is labor-consuming and several devices that enable batchwise extraction have been developed. However, these devices bear the risk of sample contamination. The present study describes a new device which improves upon two aspects of currently available devices. First, to prevent cross-sample-contamination, we redesigned the drainage module to enable independent removal of chemical waste from each individual sample funnel. Second, we added covers to the sample funnels to reduce the risk of external contamination. Cellulose purity (i.e., holocellulose) was confirmed by Fourier Transform Infrared (FTIR) Spectroscopy. Furthermore, accuracy of the radiocarbon analysis was confirmed by results of 14C-blank samples and samples of known age. In conclusion, while maintaining labor-saving, our modified device significantly reduces the risk of sample contamination during extraction of tree-ring cellulose.

The Brazil Nut tree (Bertholletia excelsa, Lecythidaceae) is a species of considerable historical, economic and ecological importance in South America. Radiocarbon dating indicates some individuals can live from hundreds to more than 1000 years, which means they have the potential to reconstruct deep time growth patterns and their relationship to anthropogenic management or climate change from pre-colonial to present times. However, age estimates vary considerably amongst trees dated with different methods (i.e. tree-ring analysis, radiocarbon-dating, and repeated diameter measurements). Here we analyze living Brazil Nut trees growing in four distinct regions across the Brazilian Amazon using two dating methods: tree-ring counting and radiocarbon dating. Our results show that the congruence between the two methods varies amongst regions, and the highest congruence is found at the site of Tefé, Amazonas. This region features archaeological sites with anthropogenic Terra Preta soils, and is known for its long-term human forest management. This management likely enhanced light and nutrient availability, which possibly enabled the trees to grow at higher rates and form annual rings. Our findings highlight the need for better understanding of the growth of Brazil Nut trees for ecological research, but also the potential of dendrochronology for exploring climate change and human-forest interactions in the Amazon Basin.

The available paleosol and paleowood data from the head of the Akkol trough valley, South Chuya range, indicates a climatically driven glacier dynamic in the Russian Altai. Radiocarbon dating of paleosols and paleotree fragments help determine the beginning of the Neoglacial in this high mountain region in the middle of the Holocene. New data limit the advance of the Sofiysky glacier at that time by the front of the Historical moraine. Less so than during the Historical stage (2.3–1.7 cal kBP), glacial activity 5–4 cal kBP is also supported by rapid reforestation. The Akkem moraine in trough valleys of the Russian Altai accumulated prior to the Holocene. The limitations and difficulties of radiocarbon dating of paleosols should be considered when interpreting the dating results.

To determine periods of incremental landslide movement and their possible relationship to regional seismic events, the tree-ring records of 32 tilted and damaged conifers at three sites on landslides in the Gravelly Range of southwestern Montana were examined. Several signs of disturbance in the tree-ring record indicating landslide movement were observed. Commonly, the tree-ring record displayed a marked reduction in annual ring width and/or the reaction wood formation. The tree-ring records from the three landslide sites indicate multiple periods of movement during the 20th century. Many of the periods of movement indicated by the strongest signals (most trees) at the sites occurred the year following significant earthquakes in the region. Those seismic events for which evidence in the tree-ring record was found at one or more of the three sites are the 1983 Borah Peak, 1959 Hebgen Lake, 1935 Helena, 1925 Clarkson, and 1908 Virginia City earthquakes. This study suggests that many of the landslide movements were triggered by, or are coincident with, earthquakes as much as 200 km from the study area.

Part of a large male woolly mammoth (Mammuthus primigenius) was preserved in permafrost in northern Yakutia. It was radiocarbon dated to ca. 18,50014C yr BP (ca. 22,500 cal yr BP). Dung from the lower intestine was subjected to a multiproxy array of microscopic, chemical, and molecular techniques to reconstruct the diet, the season of death, and the paleoenvironment. Pollen and plant macro-remains showed that grasses and sedges were the main food, with considerable amounts of dwarf willow twigs and a variety of herbs and mosses. Analyses of 110-bp fragments of the plastid rbcL gene amplified from DNA and of organic compounds supplemented the microscopic identifications. Fruit-bodies of dung-inhabiting Ascomycete fungi which develop after at least one week of exposure to air were found inside the intestine. Therefore the mammoth had eaten dung. It was probably mammoth dung as no bile acids were detected among the fecal biomarkers analysed. The plant assemblage and the presence of the first spring vessels of terminal tree-rings of dwarf willows indicated that the animal died in early spring. The mammoth lived in extensive cold treeless grassland vegetation interspersed with wetter, more productive meadows. The study demonstrated the paleoecological potential of several biochemical analytical techniques.

A dendrogeomorphological approach is presented, using wood characteristics of native oak (Quercus robur L.) to infer dynamics of aeolian sediment transport in drift-sand areas. Wood samples, taken from oaks in two drift-sand areas, were analysed to study changes in tree-ring pattern and wood anatomy as a consequence of burying or exposure from drift sand.

In all cases, the wood of the sampled oaks showed sudden changes in anatomy and tree-ring width due to burial by drift sand or subsequent exposure after erosion of the new soil surface. After aerial stems became covered by drift sand, the wood lost its characteristic ring-porous features, and tree rings became strongly reduced in width with less distinct ring boundaries. Buried stems that became exposed after erosion showed an abrupt increase in ring width and turned distinctly ring porous again. Roots that were exposed also adopted clear ring-porous features, increased in ring width and anatomically resembled aerial stem wood.

Using tree-ring analysis, it is possible to precisely date sand deposition and erosion events by detecting the concurrent changes in anatomy of woody structures. This study indicates the high potential of dendrogeomorphology as a tool to study drift-sand dynamics with a high temporal, i.e. annual, resolution for a period going back as long as the maximum age of the trees present (in this study at least 250 years). Since the signals of past deposition and erosion events are conserved in the wood, this is the only method that can be used to reconstruct drift-sand dynamics when the actual landforms are no longer present.