The IUCN Red List is widely used to guide conservation policy and practice. However, in most cases the evaluation of a species using IUCN Red List criteria takes into account only the global status of the species. Although subpopulations may be assessed using the IUCN categories and criteria, this rarely occurs, either because it is difficult to identify subpopulations or because of the effort involved. Using the jaguar Panthera onca as a model we illustrate that wide-ranging species that are assigned a particular category of threat based on the IUCN Red List criteria may display considerable heterogeneity within individual taxa in terms of the level of risk they face. Using the information available on the conservation status of the species, we evaluated the jaguar's current geographical range and its subpopulations. We identified the most threatened subpopulations, using the extent of occurrence, area of occupancy, population size and the level of threat to each subpopulation. The main outcome of this analysis was that although a large subpopulation persists in Amazonia, virtually all others are threatened because of their small size, isolation, deficient protection and the high human population density. Based on this approach, future conservation efforts can be prioritized for the most threatened subpopulations. Based on our findings we recommend that for future Red List assessments assessors consider the value of undertaking assessments at the subpopulation level. For the jaguar, sub-global assessments should be included on the Red List as a matter of urgency.

In this paper, we conduct the study of long-term age-sex-specific mortality forecasting for subpopulations in different areas of China: cities, towns and counties. We use a modified CMI (Continuous Mortality Investigation) Mortality Projections Model, which has been discussed in Huang & Browne (Paper I), for modelling purposes. From the historical experience, we find that people in cities have lower mortality rates and higher mortality improvement rates than people in towns and counties for most ages. If this trend continues, the mortality of different areas will diverge further in the future. From the projection results, we find that there will be significant mortality and life expectancy differences between cities, towns and counties for both males and females. Sensitivity analysis for long-term rates of mortality improvement and the speed of convergence from “initial” to “long-term” rates of mortality improvement are conducted. Uncertainties are attached to the central estimates to overcome the limitation of the original CMI approach from which only deterministic results can be obtained.

Monoclonal antibodies to human leucocyte antigens, including anti-CD45 and anti-CD68, have been used to describe microglia in flatmounts of normal adult human retina for the first time. Anti-CD45 (the leucocyte common antigen) intensely labeled large numbers of cells in a regular distribution across the retina; anti-CD68 and anti-macrophage antibodies labeled fewer cells with distinctive morphologies, suggesting the presence of subpopulations of microglia in the human retina expressing leucocyte antigens.