Multichannel seismic data acquired over the northern Pacific margin of the Antarctic Peninsula, have shown the presence of high-amplitude sub-bottom reflections across part of the South Shetland accretionary prism. Detailed seismic data analysis, such as true amplitude signal recovery, reflection coefficient determinations and closely-spaced semblance velocity analysis, have been carried out in order definitely to assign this bottom simulating reflector to the base of the stability field for methane hydrate. This represents the first evidence of gas hydrate layers in the Antarctic Peninsula region.