The design and experimental works of an autonomous robotic platform for road marks painting are presented in this paper as the first autonomous system of its kind. The whole system involves two main sub-systems, namely: an autonomous mobile robot navigation system which is used for recognizing the roads and estimating the position of road marks, and automatic road marks painting system that is attached to the mobile robot platform to control the spray of the paint on the road’s surface. The experimental results show the capability of the proposed system to perform the task of autonomous road marks painting with accuracy of ±10 cm.