Purple witch-weed is a parasitic plant that significantly impacts sorghum yields in semi-arid regions. It also affects crops like corn, millets, and rice. Developing purple witch-weed-resistant sorghum varieties is essential for integrated purple witch-weed management. This study evaluated the response of 48 sorghum genotypes to purple witch-weed under both pot and field conditions. Resistant varieties (Berhan and Framida) and susceptible varieties (Assosa-1, Adukara, and/or ETSL102967) were used as controls. The findings revealed substantial variability among the sorghum landraces in their response to purple witch-weed. Early maturing genotypes exhibited lower purple witch-weed densities, while late maturing ones were more susceptible. Notably, the ETSL102969 landrace showed strong resistance, comparable to the resistant variety Berhan. Additionally, the ETSL102970 landrace demonstrated superior resistance compared to Framida. Based on these results, ETSL102969 and ETSL102970 are recommended as valuable sources of resistance for breeding programs aimed at improving sorghum resistance against purple witch-weed in Ethiopia.