Although there are a number of marine organisms located from various parts of the world, little information exists from Iranian coastal waters regarding the distribution of ascidian species. Hence, the present study attempts to describe the presence and distribution of Phallusia nigra from the four different stations along Persian Gulf coastal waters. The current study is the first record of the ascidian P. nigra off the Larak Island, Hengam Island, Qeshm Island and Bandar-e-Lengeh along the Iranian coasts of the Persian Gulf.