Experiments are conducted to evaluate the tolerance of three deciduous species: black locust, honeylocust, apple, and three coniferous species: Douglas fir, ponderosa pine, and Japanese black pine, in the seedling stage to napropamide. Deciduous species were more susceptible to napropamide than coniferous species. Domestic apple had the slowest seed germination and root development and was more susceptible. The deciduous species had more secondary roots in the shallow soil layer which contained most of the herbicide than the coniferous species. The roots of all woody species tested in vitro were inhibited significantly by contact with the herbicide, but only shoot growth of domestic apple and black locust was inhibited. Injury to woody nursery crop seedlings may be avoided by delaying herbicide application.