An understanding of the growth and demise of ice sheets over North America is essential to inform future climate models. One poorly studied subject is the glacial dynamics during interstadial Marine Isotope Stage (MIS) 3 (57–29 ka). To better constrain the southern margin of the Laurentide Ice Sheet during this time period, we re-examined a stratigraphic sequence in southeast Manitoba, Canada, and provide robust evidence for advance and retreat of ice. Around 46.6 ± 5.1 ka (1σ error), fluvial sands were deposited under similar precipitation and significantly cooler summer temperatures than present-day. Ice then advanced south over the area, before retreating once again and a return to boreal forest and grassland conditions. The area was then covered by proglacial Lake Vita, dammed by ice to the north. Geochronology constraints indicate Lake Vita existed from ca. 44.3 ± 3.6 to 30.4 ± 2.3 ka (1σ error), although gaps in the optical and finite radiocarbon ages suggest either a lack of data or plausible temporary ice-margin advances during this time period. Ice covering most of Manitoba during MIS 3 is in line with global δ18O records, and glacially influenced sediment deposition in the Mississippi River basin.

Detailed reconstructions of the vegetation of Iberia during the last glacial inception are rare due to the limited number of terrestrial sites recording this period. Active retreat of El Asperillo cliff, located on the Atlantic coast of southwestern Iberia, has exposed a fossil organic level dating back to one of the early stades of the last glacial cycle. Pollen and macrofossil analyses from this site show that the Doñana area was covered mainly by steppic vegetation; temperate trees survived the coldest periods, albeit in reduced numbers. Mediterranean taxa are extremely reduced, in contrast with other dry areas of southern Iberia over this time span. This vegetation suggests cold and arid climatic conditions, in accordance with paleoclimatic reconstructions based on several Atlantic marine cores.

Macro- and megafossil studies are of great value in palaeoecology because such evidence is spatially precise, directly radiocarbon dated, and usually taxon-specific. Here, we present new macro- and megafossil data from ten sites from the Gredos Mountains, central Iberian Peninsula, that suggest persistent forest cover through the late Holocene, with a widespread belt of pinewoods in the highlands of the Central Iberian Mountains. Well-preserved pine cones found at several sites revealed that both Pinus sylvestris and Pinus nigra were present in the area during the middle and late Holocene at locations of important biogeographical interest. The P. sylvestris forests represent one of the southernmost locations of its entire range. P. nigra was not known to have occurred in central Spain during the Holocene; it was found at the westernmost edge of its range in siliceous soils, a rare environment compared with the rest of its distribution. Finally, we explored the potential for obtaining a long pine chronology from central Iberia using tree-ring measurements and radiocarbon dating of pine subfossil logs.