Haloxyfop-methyl toxicity to johnsongrass was reduced when sprayed in combination with 2,4-D in greenhouse studies. The antagonism was not seen when 2,4-D application followed haloxyfop-methyl treatment by 72 h or preceded it by 24 h. The antagonism of haloxyfopmethyl activity was evident regardless of chemical form of 2,4-D used (DMA, BOE ester, IPA, or acid). Absorption of 14C-haloxyfop-methyl into johnsongrass leaves was slowed by the presence of 2,4-D in the treatment solution but total absorption after 72 h was not affected. Translocation of 14C was decreased when the 14C-haloxyfop-methyl was applied in combination with 2,4-D. More haloxyfop-methyl, less haloxyfop, and more ether-soluble haloxyfop-methyl metabolites were found in treated leaves when haloxyfop-methyl was applied with 2,4-D compared to haloxyfop-methyl applied alone. The changes in haloxyfop-methyl translocation and metabolism caused by 2,4-D can quantitatively account for the antagonism observed.