This paper presents results of accelerator mass spectrometry radiocarbon (AMS 14C) dating of prehistoric samples (human and animal bones, cremated human bones, charcoals, and other charred plant macroremains) from archaeological sites located in the area of Dobużek Scarp, on the Sokal Ridge in central-eastern Poland (E Poland). The date list reports 46 14C age measurements performed within the project “The Dobużek Scarp Microregion as a part of a physiological and biocultural frontier between the Baltic and the Pontic zone (from the 6th to the 2nd millennium BC)” conducted in 2016–2021. The resulting 14C dates fall into quite a long interval, which in terms of the regional archaeological periodization lasts from the Middle Eneolithic to the Early Iron Age, and in terms of the climatological one corresponds with the Subboreal.