Ice-wedge activity can be used to reconstruct past environmental conditions. We investigated the moisture source and timing of ice-wedge formation on the Blackstone Plateau. A section of permafrost exposed ice wedges that developed at two distinct depths: the first set formed syngenetically and penetrated alluvial silts from the top of permafrost; the second set, truncated by an erosional or thaw contact, was found solely in icy muddy gravels (>3.1 m depth). The δ18O and D-excess records of the ice wedges suggest that they formed from freezing of snow meltwater whose isotopic composition evolved during meltout. The 14CDOC results suggest that climate was favorable to ice-wedge growth between 32,000–30,000 and 14,000–12,500 cal yr BP, but there was likely a hiatus during the last glacial maximum due to climate being too dry. During the early to mid-Holocene, ice wedges were inactive as a result of warmer and wetter climate. Ice wedge re-initiated around 6360 cal yr BP, with a peak in activity between 3980 and 920 cal yr BP, a period characterized by cool and moist climate. Overall, timing of ice-wedge activity was broadly consistent with the climate and vegetation evolution in the western Arctic.