A screening method was used to characterize seed thermal responses of prostrate knotweed and common purslane, two important weeds invading wheat in the humid Pampa. Through this method, it was possible to detect thermal conditions that induce or break dormancy in both species. In addition, we were able to quantify changes in dormancy level in seed populations as a function of time of burial after dispersal, through changes in width of the thermal range within which germination can occur. Plotting the overlap of this thermal range and observed soil temperature throughout the year allowed the prediction of the seedling emergence period. This prediction was in agreement with observed seedling emergence in the field for both species, during 2 consecutive yr. From the analysis carried out under laboratory conditions, it was also possible to estimate required thermal time for germination of the nondormant fraction of the population and the base temperature above which thermal time is accumulated. The results obtained from this study are the basis for the formulation of seed germination models that predict not only the occurrence of seedling emergence in the field, but also the dynamics of germination within those periods.

In a semiarid Mediterranean site in central Spain, field experiments were conducted on a Calcic Haploxeralf (noncalcic brown soil), which had been managed with three crop rotations and two tillage systems (no-tillage and conventional tillage) since 1987. The crop rotations consisted of barley→vetch, barley→sunflower, and a barley monoculture. The study took place in two growing seasons (1992–1994) to assess the effects of management practices on the weed seedbank. During this period, spring weed control was not carried out in winter crops. In the no-tillage system, there was a significant increase in the number of seeds of different weed species: anacyclus, common purslane, corn poppy, knotted hedge-parsley, mouse-ear cress, spring whitlowgrass, tumble pigweed, venus-comb, and Veronica triphyllos. Conversely, the presence of prostrate knotweed and wild radish was highest in plots under conventional tillage. These results suggest large differences in the weed seedbank as a consequence of different soil conditions among tillage systems, but also the necessity of spring weed control when a no-tillage system is used. With regard to crop rotations, the number of seeds of knotted hedge-parsley, mouse-ear cress, and spring whitlowgrass was greater in the plots under the barley→vetch rotation. Common lambsquarters dominated in the plots under the barley→sunflower rotation, whereas venus-comb was the most frequent weed in the barley monoculture. Larger and more diverse weed populations developed in the barley→vetch rotation rather than in the barley→sunflower rotation or the barley monoculture.

Replacement series studies were conducted under controlled conditions to determine the effect of phosphorus (P) rates and population densities on the competitiveness of smooth pigweed and common purslane with lettuce. Densities were 2, 4, and 8 plants per 113 cm2, whereas P rates were 0, 0.4, and 0.8 g PL−1 soil. A P-deficient Histosol (0.3 mg water-extractable P L−1 soil) was used. High P fertility enhanced the competitive ability of lettuce in smooth pigweed-lettuce mixtures. Smooth pigweed was not responsive to P rates. However, luxurious P consumption by smooth pigweed occurred, reducing the amount of the nutrient available for lettuce absorption. In common purslane-lettuce mixtures, the weed was responsive to P rates, increasing its competitive ability, whereas no increase in lettuce competitive ability was observed. Both weed species were more competitive than lettuce. Competition for P appears to be the main mechanism of common purslane interference on lettuce grown in low-P organic soils. Alternative fertilization strategies (i.e., banded applications) may reduce the effect of smooth pigweed on lettuce.

Weed seeds can require an exposure to light for induction of germination. Conducting tillage operations at night and thus preventing the photoinduction of germination has been proposed as a means to reduce weed emergence in agricultural systems. This research was conducted to evaluate night tillage as a weed management option and to determine which tillage operations have the greatest effect on light-mediated recruitment. Weed emergence was evaluated after conducting factorial combinations of day and night moldboard plowing and disking in the springtime from 1992 through 1995. The light environment during disking generally had a slightly greater effect on emergence than the light environment during plowing. Emergence of pigweed species and giant foxtail was, at most, 30 to 55% higher following day vs. night disking. Emergence of other weeds was not affected by the light environment during tillage. We conclude that night tillage may not be a viable approach to weed management due to insufficient reductions in weed emergence associated with night tillage and the high degree of variability in the recruitment response to light conditions during tillage.

Field experiments, conducted from 1991 to 1994, generated information on weed seedbank emergence for 22 site-years from Ohio to Colorado and Minnesota to Missouri. Early spring seedbank densities were estimated through direct extraction of viable seeds from soil cores. Emerged seedlings were recorded periodically, as were daily values for air and soil temperature, and precipitation. Percentages of weed seedbanks that emerged as seedlings were calculated from seedbank and seedling data for each species, and relationships between seedbank emergence and microclimatic variables were sought. Fifteen species were found in 3 or more site-years. Average emergence percentages (and coefficients of variation) of these species were as follows: giant foxtail, 31.2 (84%); velvetleaf, 28.2 (66); kochia, 25.7 (79); Pennsylvania smartweed, 25.1 (65); common purslane, 15.4 (135); common ragweed, 15.0 (110); green foxtail, 8.5 (72); wild proso millet, 6.6 (104); hairy nightshade, 5.2 (62); common sunflower, 5.0 (26); yellow foxtail, 3.4 (67); pigweed species, 3.3 (103); common lambsquarters, 2.7 (111); wild buckwheat, 2.5 (63), and prostrate knotweed, 0.6 (79). Variation among site-years, for some species, could be attributed to microclimate variables thought to induce secondary dormancy in spring. For example, total seasonal emergence percentage of giant foxtail was related positively to the 1st date at which average daily soil temperature at 5 to 10 cm soil depth reached 16 C. Thus, if soil warmed before mid April, secondary dormancy was induced and few seedlings emerged, whereas many seedlings emerged if soil remained cool until June.

The critical period for weed control (CPWC) identifies the phase of the crop growth cycle when weed interference results in unacceptable yield losses; however, the effect of planting date on CPWC is not well understood. Field studies were conducted in 2004 and 2005 at Urbana, IL, to determine CPWC in sweet corn for early May (EARLY) and late-June (LATE) planting dates. A quantitative series of treatments of both increasing duration of interference and length of weed-free period were imposed within each planting-date main plot. The beginning and end of the CPWC, based on 5% loss of marketable ear mass, was determined by fitting logistic and Gompertz equations to the relative yield data representing increasing duration of weed interference and weed-free periods, respectively. Weed interference stressed the crop more quickly and to a greater extent in EARLY, relative to LATE. At a 5% yield-loss level, duration of weed interference for 160 and 662 growing-degree days (GDD) from crop emergence marked the beginning of the CPWC for EARLY and LATE, respectively. When maintained weed-free for 320 and 134 GDD, weeds emerging later caused yield losses of less than 5% for EARLY and LATE, respectively. Weed densities exceeded 85 plants m−2 for the duration of the experiments and predominant species included barnyardgrass, common lambsquarters, common purslane, redroot pigweed, and velvetleaf. Weed canopy height and total aboveground weed biomass were 300% and 500% higher, respectively, for EARLY compared with LATE. Interactions between planting date and CPWC indicate the need to consider planting date in the optimization of integrated weed management systems for sweet corn. In this study, weed management in mid-June–planted sweet corn could have been less intensive than early May–planted corn, reducing herbicide use and risk of herbicide carryover to sensitive rotation crops.

Three years of field trials have been carried out in Zaragoza, Spain, using different biodegradable mulch materials in processing tomatoes. The aim was to evaluate weed control with several biodegradable mulches as alternatives to black polyethylene (PE) mulch. The treatments were rice straw, barley straw, maize harvest residue, absinth wormwood plants, black biodegradable plastic, brown kraft paper, PE, herbicide, manual weeding, and unweeded control. Assessments focused on weeds and on crop yield. A laboratory study showed that 1 kg/m2 of organic mulch was sufficient to cover the soil for rice, barley straw, and maize harvest residue. The most abundant weed species in the field were purple nutsedge, common purslane, common lambsquarters, and large crabgrass and a change in weed composition was observed between treatments and years. Most weed species were controlled by the mulching materials except that purple nutsedge was controlled only by paper mulch. The other species were well controlled by PE and biodegradable plastic and also by some of the organic mulch treatments. Best weed control and lowest weed biomass were achieved by paper followed by PE and biodegradable plastic. The best organic mulch was rice straw and the worst weed control was from absinth wormwood. Tomato yield was highest for PE followed by paper, manual weeding, biodegradable plastic, and rice straw and was clearly related to weed control. Paper, biodegradable plastic, and rice straw are potential substitutes for PE and herbicides.

The growth habits of the crop and competing weed species are important determinants of crop–weed competition. Three cowpea genotypes with similar vegetative vigor but different growth habit were assessed for their relative competitiveness with two weed species. ‘Iron-Clay’ (IC) grows erect, ‘IT89KD-288’ (288) is semierect, and ‘UCR 779’ (779) is prostrate. Common purslane, a short-statured weed, and common sunflower, a tall species, were planted within the cowpea rows. Cowpea canopy height and width, leaf area, dry weight, and light intensity above and below sunflower and cowpea canopies were measured weekly from 21 d after planting. Sunflower reduced the leaf area, amount of light received, and biomass of all cowpea genotypes. Purslane reduced the leaf area of 779 and the biomass of 288 and 779, but the biomass and leaf area of IC were not affected. The presence of sunflower increased the height of IC and 288, but the presence of purslane decreased the canopy height of 779. IC reduced sunflower biomass, whereas IC and 779 reduced purslane biomass. IC and 288 reduced sunflower leaf area, whereas IC and 779 reduced purslane leaf area. The growth analysis of biomass, leaf area, and canopy height of cowpeas and weeds showed similar results. The experiments indicate that cowpea genotypes differ in their ability to compete with purslane and sunflower. IC was the most competitive genotype, suggesting that an erect growth habit may be more effective in suppressing weeds than semierect or prostrate growth habits.

Organic vegetable producers have limited options for managing weeds. They cite weed management as their number one research priority. Studies were conducted in 2004 and 2005 at the Black Sea Agricultural Research Institute, Samsun, Turkey, to determine the weed suppressive effects of summer cover crops in organic kale production. Treatments consisted of grain sorghum, sudangrass, hairy vetch, grain amaranth, pea, and fallow. Weed density and total weed dry biomass were assessed before and at 14, 28, and 56 d after incorporation (DAI) of the cover crops. Kale was transplanted 14 DAI and hand weeded once after last weed evaluation (56 DAI). All cover crops produced at least 1 ton/ha (t/ha) biomass; grain sorghum produced more dry matter than all other cover crops in both years. After incorporation of the cover crops, hairy vetch and sorghum treatments showed fewer species, lower weed density, and total weed dry biomass compared with other treatments. Cover crops suppressed emergence of common purslane, common lambsquarters, redroot pigweed, European heliotrope, field pennycress, annual sowthistle, black nightshade, shepherd's-purse, wild mustard, sun spurge, Persian speedwell, annual mercury, and jimsonweed up to 56 DAI. Total kale yield in hairy vetch treatments was more than double that of the no cover crop, and was significantly higher than yield from the other cover crop treatments. These results indicate that hairy vetch, grain sorghum, and sudangrass have ability to suppress early-season weeds in organic kale production.

Greenhouse studies were conducted to assess the intensity of smooth pigweed and common purslane aboveground interference (AI) and belowground interference (BI) with lettuce and to determine primary mechanisms of interference of each species as affected by P fertility rates. Lettuce was transplanted in mixtures with either smooth pigweed or common purslane according to four partitioning regimes: no interference, full interference, BI, and AI. Soil used was low in P for optimum lettuce yields, therefore P was added at rates of 0, 0.4, and 0.8 grams of P per liter of soil. Shoot and root biomass and plant height were measured for each species, as well as P tissue content. The data obtained indicated that smooth pigweed interfered with lettuce primarily through light interception by its taller canopy. A secondary mechanism of interference was the absorption of P from the soil through luxury consumption, increasing the P tissue content without enhancing smooth pigweed biomass accumulation. In contrast, common purslane competed aggressively with lettuce for P. Because the weed grew taller than lettuce, light interception was a secondary interference factor.

Abrasive weed control is a novel weed management tactic that has great potential to increase the profitability and sustainability of organic vegetable cropping systems. The objective of this study was to determine the effect of air-propelled organic abrasive grits (e.g., organic fertilizers) on weed seedling emergence and growth and vegetable crop growth. A series of thirteen greenhouse trials were conducted to determine the susceptibility of weeds to abrasive weed control with one of six organic materials including: corn cob grits, corn gluten meal, greensand fertilizer, walnut shell grits, soybean meal, and bone meal fertilizer. In addition, crop injury was quantified to determine the potential utility of each organic material as abrasive grits in tomato and pepper cropping systems. Of the six organic materials, corn gluten meal, greensand fertilizer, walnut shell grits, and soybean meal provided the broadest range of POST weed control. For example, one blast of corn gluten meal and greensand fertilizer reduced Palmer amaranth (one-leaf stage) seedling biomass by 95 and 100% and green foxtail (one-leaf stage) biomass by 94 and 87%, respectively. None of the organic materials suppressed weed seedling emergence when applied to the soil surface, suggesting that residual weed control with abrasive grits is unlikely. Tomato and pepper stems were relatively tolerant of abrasive grit applications, though blasting with select materials did increase stem curvature in tomato and reduced biomass (corn cob grit) and relative growth rate (corn gluten meal and greensand) in pepper. Results suggest that organic fertilizers can be effectively used as abrasive grits in vegetable crops, simultaneously providing weed suppression and supplemental crop nutrition. Field studies are needed to identify cultural practices that will increase the profitability of multifunctional abrasive weed control in organic specialty crops.

Laboratory studies were conducted to examine the leaf surface, epicuticular wax content, and spray droplet behavior on common lambsquarters, common purslane, and velvetleaf. Adaxial and abaxial leaf surfaces were examined using scanning electron microscopy, and leaf wax was extracted and quantified for all three weed species. The spread of 1-μl droplets of distilled water, primisulfuron solution (without surfactant), primisulfuron solution with a nonionic low foam wetter/spreader adjuvant (0.25% v/v), and with an organosilicone wetting agent (0.1% v/v) was determined on the adaxial leaf surfaces of each of the weed species. Glands and trichomes were present on both the adaxial and abaxial leaf surfaces of velvetleaf. Common purslane had neither glands nor trichomes on either side of the leaf. Common lambsquarters did not have any glands or trichomes, but it had globular bladder hairs on both adaxial and abaxial leaf surfaces. Stomata were present on both adaxial and abaxial leaf surfaces in all three weed species. Common purslane had a much lower number of stomata per unit area of leaf as compared with velvetleaf or common lambsquarters. Common lambsquarters had the highest epicuticular wax content on the leaf surface (274.5 μg cm−2), followed by common purslane (153.4 μg cm−2) and velvetleaf (7.4 μg cm−2). There were no significant variations in the spread of the 1-μl droplet of distilled water and primisulfuron (without adjuvant) among the species. Spread of primisulfuron droplets with surfactant was highest on the leaf surface of velvetleaf that had the lowest wax content. Droplet spread was greatest with organosilicone surfactant followed by the nonionic surfactant.

Crops that effectively compete with weeds may be more suitable in low-input agricultural systems. A 2-yr field experiment was conducted in northern Greece to assess the competitiveness of seven annual aromatic plants (anise, sweet fennel, sweet basil, dill, coriander, parsley, and lacy phacelia) on common purslane, common lambsquarters, black nightshade, and barnyardgrass. The phytotoxicity of the essential oils produced by these aromatic plants was also determined using a perlite-based bioassay with barnyardgrass. Separation, identification, and quantification of the volatile compounds of these essential oils were also performed. After the harvest of the aromatic plants (8 wk after planting), the greatest weed fresh weight reduction (94 to 100%) was recorded in lacy phacelia, whereas the least (0 to 30%) was recorded in parsley. Lacy phacelia and sweet fennel produced the greatest fresh biomass yield in weedy and weed-free treatments, whereas parsley, dill, and coriander produced the lowest. Biomass of sweet fennel and anise was reduced by only 9 to 11% by weed competition, whereas biomass of lacy phacelia was not significantly affected. The essential oils isolated from sweet fennel and sweet basil were the most phytotoxic on barnyardgrass, whereas those isolated from lacy phacelia and anise were the least phytotoxic. Conclusively, aromatic plants with great competitiveness such as lacy phacelia, anise, and sweet fennel provided great weed suppression and they could be cultivated with low inputs in herbicides. However, high competitiveness of aromatic plants may not always be correlated with high essential oil phytotoxicity.

Field studies were carried out in Laingsburg, MI, from 2002 to 2004 on Houghton muck soil to assess the impacts of cover crops and soil fertility regimes on weed populations and celery yield. The cover crops were oilseed radish, cereal rye, hairy vetch, and a bare ground control. The fertility rates were full (180, 90, and 450 kg ha−1 nitrogen [N], phosphorus pentoxide [P2O5], and potassium oxide [K2O], respectively), half (90, 45, and 225 kg ha−1 N, P2O5, and K2O, respectively), and low (90 kg ha−1 N). Each cover crop treatment was combined with the low or half rate of fertilizer. An additional treatment with bare ground plus the full rate of fertilizer was added as standard practice. Treatments were maintained in the same location for the duration of the study. Major weed species were common chickweed, prostrate pigweed, shepherd's-purse, common purslane, and yellow nutsedge. Each year, oilseed radish consistently produced the greatest biomass and provided over 98% early season weed biomass suppression. Hairy vetch and cereal rye provided about 70% weed suppression in early spring. Soil fertility level affected weed populations during the 2004 growing season. In 2004, weed biomass in treatments without cover crops or with vetch increased when greater amounts of fertilizer were applied. Within individual fertility levels, higher celery yields were recorded in the oilseed radish plots. For example, in the low fertility rate, celery yield was 34.8, 29.2, 23.9, and 24.4 ton ha−1 in the oilseed radish, cereal rye, hairy vetch, and control plots, respectively in 2003. Overall, the results of this experiment indicate that when included in a system where hoeing and hand-weeding are the only weed control methods, cover crops can successfully improve weed management and celery yield on muck soils, allowing reduced fertilizer inputs.

Weeds that emerge between rows in fresh market tomatoes after the critical period of competition are not suppressed by the crop and can produce large quantities of seed. A living mulch planted between rows might limit weed seed production. Buckwheat was seeded between tomato rows after the critical period in 2007 and 2008 in field studies near Lafayette, IN. Weeds were allowed to emerge after the critical period (CP), controlled throughout the growing season (no seed threshold [NST]), or mowed to limit seed production (MOW). Buckwheat and MOW plots were mowed twice after the critical period in 2007 and once in 2008. Seed banks were sampled after the critical period and in the following spring. Tomato yields were not reduced by growing buckwheat between rows. Seed bank densities for common purslane and carpetweed, which escaped mowing due to their prostrate habits, increased in all treatments. Germinable seed bank densities were 306 seeds m−2 or less in the NST and buckwheat treatments but 755 seeds m−2 or more in the CP treatments for species with erect habits in both years. Seed bank densities were lower in the MOW treatment than in the CP treatments in 2007 but not in 2008. In a parallel experiment conducted in adjacent plots, buckwheat was seeded at five rates (0, 56, 112, 168, and 224 kg seed ha−1). Plots were mowed and emergent weeds sampled as described for the intercrop experiment. Weed densities before mowing decreased linearly with buckwheat seed rate. After mowing, no relationship was detected between seed rate and weed densities. This study supports the hypothesis that a living mulch planted after the critical period can be used to limit seed bank growth without reducing tomato yields, but additional research is needed to better understand the effect of mowing on living mulch growth and weed suppression.

Trials were carried out to investigate the effects of seed burial depth on seedling emergence rate of 20 weed species. Marked depth-mediated variation in emergence ability of the different species was observed, together with a general pattern of decreasing emergence with increasing soil depth. At 10 cm, only johnsongrass, velvetleaf, catchweed bedstraw, and cutleaf geranium emerged, albeit only in limited numbers. Species most severely inhibited by burial depth were buckhorn plantain, large crabgrass, common purslane, chickweed, and corn spurry, none of which emerged from beyond 6 cm. In all species, depth-mediated inhibition was found to be sigmoidal (polynomial regression). In addition, the number of seedlings and rate of seedling emergence decreased when depth of burial increased. The depth at which the number of emerged seedlings was halved varied by species and ranged from 3.6 cm for common purslane and chickweed to 7 cm for velvetleaf and catchweed bedstraw. Excessive burial depth generally induced dormancy (in roughly 85% of cases) rather than suicide germination. A close inverse relation (second-degree equation) between seed unit weight and depth-mediated inhibition was observed. The physiological involvement of depth inhibition in seed bank ecology is discussed.

There are limited PRE herbicide options available to provide residual weed control in sweet corn grown on organic soils in the Everglades Agricultural Area (EAA). Field studies were established to determine the efficacy of PRE applied saflufenacil + dimethenamid-P at six rates ranging from 10 + 88 to 319 + 2802 g ai ha−1 on weed control and sweet corn tolerance on organic soils in the EAA in 2011 and 2012. Saflufenacil + dimethenamid-P is a premix recently labeled for PRE weed control in field corn at 50 + 438 to 90 + 788 g ha−1 depending on soil texture. There was no phytotoxic effect of PRE applied saflufenacil + dimethenamid-P on sweet corn. At 42 d after treatment, common lambsquarters, common purslane, and spiny amaranth were controlled 90% with saflufenacil + dimethenamid-P at 58 + 508, 71 + 622, and 58 + 512 g ha−1, respectively. Sweet corn yield at 95% of the weed-free yield was estimated to be obtained at 69 + 606 g ha−1 of saflufenacil + dimethenamid-P. Our results show that saflufenacil + dimethenamid-P at 69 + 606 to 71 + 622 g ha−1 controlled three common weeds and maintained acceptable sweet corn yield. Labeled rates of saflufenacil + dimethenamid-P for field corn on mineral soils were adequate for weed control in sweet corn on organic soils.

The weed management needs of organic producers are unique because they rely primarily on cultural and physical management strategies. Recommendations regarding commonly used tools for weed management could benefit this sector of agriculture. The objectives of this research were to (1) determine the optimum time of day for propane flaming to achieve maximum weed reductions while minimizing corn damage; (2) assess whether flaming, rotary hoeing, or a combination of the two tools best manages early-season weeds without injuring dry beans; and (3) evaluate the use of growing degree days (GDD) to optimize rotary hoe timing. Experiments were carried out between 2006 and 2009 in Hickory Corners and East Lansing, MI. Flaming reduced broadleaf weed densities by at least 82% when done in the morning to midafternoon but only reduced densities by 58% when weeds were flamed in the evening. Common lambsquarters, redroot pigweed, and velvetleaf were easier to control by flaming than common ragweed and common purslane. Flaming did not reduce grass weed densities. When comparing flaming and rotary hoeing, the two treatments that achieved the highest level of weed control and highest dry bean yields were flaming prior to bean emergence followed by two rotary hoeings and rotary hoeing three times (no flaming). However, the added cost of the flamer may only be justified when wet conditions make rotary hoeing ineffective. Flaming dry beans POST resulted in significant injury and yield reductions of 60%; therefore this practice is not recommended. Timing rotary hoe passes every 300 GDD (base 3.3 C) from the time of soybean or dry bean planting resulted in fewer passes compared with the 7-d or 150 GDD treatments, while maintaining similar levels of weed control and yields similar to the weed-free treatment in 1 of 2 yr for each crop.

Mortality of weed seeds at temperatures of 39, 42, 46, 50, 60, and 70 C was recorded through time under controlled laboratory conditions similar to those of soil solarization for six weed species: annual sowthistle, barnyardgrass, black nightshade, common purslane, London rocket, and tumble pigweed. Time and temperature requirements for thermal death varied considerably among the species studied. Barnyardgrass, London rocket, and annual sowthistle were more susceptible to heat treatment than black nightshade, common purslane, and tumble pigweed. Temperatures of 50 C and above were lethal for seeds of all species. Common purslane seeds were unaffected at 46 C and below, tumble pigweed and barnyardgrass seeds were unaffected at 42 C and below, and black nightshade seeds were unaffected at 39 C. Nonlinear models for mortality as a function of duration of heat treatment were developed for each species at each temperature at which mortality occurred. These models provide an empirical relationship for the construction of field-applicable decision models that could predict the accumulation of time and temperature combinations for effective solarization of weed seeds.

The root exudates produced by sorghums contain a biologically active constituent known as sorgoleone. Seven sorghum accessions were evaluated for their exudate components. Except for johnsongrass, which yielded 14.8 mg root exudate/g fresh root wt, sorghum accessions consistently yielded approximately 2 mg root exudate/g fresh root wt. Exudates contained four to six major components, with sorgoleone being the major component (> 85%). Three-dimensional structure analysis was performed to further characterize sorgoleone's mode of action. These studies indicated that sorgoleone required about half the amount of free energy (493.8 kcal/mol) compared to plastoquinone (895.3 kcal/mol) to dock into the QB-binding site of the photosystem II complex of higher plants. Light, cryo-scanning, and transmission electron microscopy were utilized in an attempt to identify the cellular location of root exudate production. From the ultrastructure analysis, it is clear that exudate is being produced in the root hairs and being deposited between the plasmalemma and cell wall. The exact manufacturing and transport mechanism of the root exudate is still unclear. Studies were also conducted on sorgoleone's soil persistence and soil activity. Soil impregnated with sorgoleone had activity against a number of plant species. Recovery rates of sorgoleone-impregnated soil ranged from 85% after 1 h to 45% after 24 h. Growth reduction of 9 14-d-old weed species was observed with foliar applications of sorgoleone.