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Dietary inclusion of Clostridium butyricum cultures alleviated impacts of high-carbohydrate diets in largemouth bass (Micropterus salmoides)

Published online by Cambridge University Press:  11 December 2023

Jiajie Tao ,
Ye Gong ,
Shiwen Chen ,
Wenfei Li ,
Ruitao Xie ,
Haitao Zhang ,
Naisong Chen ,
Xuxiong Huang  and
Songlin Li Open the ORCID record for Songlin Li [Opens in a new window]
Jiajie Tao
Affiliation:
Research Centre of the Ministry of Agriculture and Rural Affairs on Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, People’s Republic of China
Ye Gong
Affiliation:
Research Centre of the Ministry of Agriculture and Rural Affairs on Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, People’s Republic of China
Shiwen Chen
Affiliation:
Research Centre of the Ministry of Agriculture and Rural Affairs on Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, People’s Republic of China
Wenfei Li
Affiliation:
Research Centre of the Ministry of Agriculture and Rural Affairs on Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, People’s Republic of China
Ruitao Xie
Affiliation:
Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture and Rural Affairs, Zhanjiang, People’s Republic of China
Haitao Zhang
Affiliation:
Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture and Rural Affairs, Zhanjiang, People’s Republic of China
Naisong Chen
Affiliation:
Research Centre of the Ministry of Agriculture and Rural Affairs on Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, People’s Republic of China National Demonstration Center on Experiment Teaching of Fisheries Science, Shanghai Ocean University, Shanghai, People’s Republic of China
Xuxiong Huang
Affiliation:
Research Centre of the Ministry of Agriculture and Rural Affairs on Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, People’s Republic of China National Demonstration Center on Experiment Teaching of Fisheries Science, Shanghai Ocean University, Shanghai, People’s Republic of China
Songlin Li*
Affiliation:
Research Centre of the Ministry of Agriculture and Rural Affairs on Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, People’s Republic of China National Demonstration Center on Experiment Teaching of Fisheries Science, Shanghai Ocean University, Shanghai, People’s Republic of China
*
*Corresponding author: Dr S. Li, fax +86 21 61900465, email slli@shou.edu.cn
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Abstract

A 60-d feeding trial was conducted to explore the potential regulatory effects of dietary Clostridium butyricum cultures (CBC) supplementation in high-carbohydrate diet (HCD) on carbohydrate utilisation, antioxidant capacity and intestinal microbiota of largemouth bass. Triplicate groups of largemouth bass (average weight 35·03 ± 0·04 g), with a destiny of twenty-eight individuals per tank, were fed low-carbohydrate diet and HCD supplemented with different concentration of CBC (0 %, 0·25 %, 0·50 % and 1·00 %). The results showed that dietary CBC inclusion alleviated the hepatic glycogen accumulation induced by HCD intake. Additionally, the expression of hepatic ampkα1 and insulin signaling pathway-related genes (ira, irb, irs, p13kr1 and akt1) increased linearly with dietary CBC inclusion, which might be associated with the activation of glycolysis-related genes (gk, pfkl and pk). Meanwhile, the expression of intestinal SCFA transport-related genes (ffar3 and mct1) was significantly increased with dietary CBC inclusion. In addition, the hepatic antioxidant capacity was improved with dietary CBC supplementation, as evidenced by linear decrease in malondialdehyde concentration and expression of keap1, and linear increase in antioxidant enzyme activities (total antioxidative capacity, total superoxide dismutase and catalase) and expression of antioxidant enzyme-related genes (nrf2, sod1, sod2 and cat). The analysis of bacterial 16S rRNA V3–4 region indicated that dietary CBC inclusion significantly reduced the enrichment of Firmicutes and potential pathogenic bacteria genus Mycoplasma but significantly elevated the relative abundance of Fusobacteria and Cetobacterium. In summary, dietary CBC inclusion improved carbohydrate utilization, antioxidant capacity and intestinal microbiota of largemouth bass fed HCD.

Keywords

Clostridium butyricum culturesLargemouth bassInsulin signalling pathwayAntioxidant capacityIntestinal microbiota
Type
Research Article
Information
British Journal of Nutrition , Volume 131 , Issue 8 , 28 April 2024 , pp. 1308 - 1325
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Copyright
© The Author(s), 2023. Published by Cambridge University Press on behalf of The Nutrition Society

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