Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-23T06:40:04.349Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of soya oligosaccharides and soya oligopeptides on lipid metabolism in hyperlipidaemic rats

Published online by Cambridge University Press:  15 November 2011

Shali Xie ,
Jundong Zhu ,
Yanqi Zhang ,
Kai Shi ,
Yuangang Shi  and
Xiao Ma
Shali Xie
Affiliation:
West China School of Public Health, Sichuan University, No. 17, Section 3, South Renmin Road, Chengdu, Sichuan610041, People's Republic of China Department of Health Education and Medical Humanities, College of Humanities and Social Sciences, Third Military Medical University, No. 30 Gaotanyan Road, Chongqing400038, People's Republic of China
Jundong Zhu*
Affiliation:
Department of Nutrition and Food Hygiene, College of Military Preventive Medicine, Third Military Medical University, No. 30 Gaotanyan Road, Chongqing400038, People's Republic of China
Yanqi Zhang
Affiliation:
Department of Health Statistics, College of Military Preventive Medicine, Third Military Medical University, No. 30 Gaotanyan Road, Chongqing400038, People's Republic of China
Kai Shi
Affiliation:
Department of Health Education and Medical Humanities, College of Humanities and Social Sciences, Third Military Medical University, No. 30 Gaotanyan Road, Chongqing400038, People's Republic of China
Yuangang Shi
Affiliation:
Department of Nutrition and Food Hygiene, College of Military Preventive Medicine, Third Military Medical University, No. 30 Gaotanyan Road, Chongqing400038, People's Republic of China
Xiao Ma*
Affiliation:
West China School of Public Health, Sichuan University, No. 17, Section 3, South Renmin Road, Chengdu, Sichuan610041, People's Republic of China
*
*Corresponding authors: Professor J. Zhu, email [email protected]; Professor X. Ma, fax +86 28 5585080, email [email protected]
*Corresponding authors: Professor J. Zhu, email [email protected]; Professor X. Ma, fax +86 28 5585080, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

In the present study, we aimed to examine the effects of soya oligosaccharides (SOS) and soya oligopeptides (SOP) on blood lipid levels, release of vasoactive substances, antioxidant activity and faecal bile acid (FBA) excretion in rats fed a high-fat diet (HFD). Male Sprague–Dawley rats were evenly divided into five groups according to diets as follows: regular diet (control), HFD, HFD enriched with 2 % of SOS (SOS), HFD enriched with 3 % of SOP (SOP) and HFD enriched with 2 % SOS and 3 % SOP (SOSP). The results showed that SOS and SOP significantly reduced plasma total cholesterol, LDL-cholesterol and TAG, whereas HDL-cholesterol concentration was significantly increased. Furthermore, SOS and SOP reduced plasma apoB, apoE and the apoB:apoAI ratio, whereas apoAI was significantly increased. Moreover, SOS and SOP also reduced plasma thromboxane A2 (TXA2) and the TXA2:prostacyclin (PGI2) ratio, whereas plasma PGI2 and nitric oxide were significantly increased. In addition, SOS and SOP significantly reduced serum and liver malondialdehyde concentrations and increased FBA excretion. However, we did not observe obvious influences of SOS and SOP on superoxide dismutase activities in the liver of HFD-fed rats. The combination of 2 % SOS and 3 % SOP showed a more marked effect than SOS or SOP alone in improving the lipid profile, release of vasoactive substances and increasing FBA excretion (P < 0·05). In summary, SOS and SOP might help prevent atherosclerosis through improving abnormal blood lipid levels, regulating vasoactive substances and protecting against oxidative stress.

Keywords

Soya oligosaccharidesSoya oligopeptidesLipid metabolismAtherosclerosis
Type
Full Papers
Information
British Journal of Nutrition , Volume 108 , Issue 4 , 28 August 2012 , pp. 603 - 610
Copyright
Copyright © The Authors 2011

CVD is the leading cause of mortality in the world, accounting for the deaths of seventeen million people each year or approximately one-third (29 %) of global deaths annually(1). CVD prevention is a global public health priority. According to the estimation of the WHO, 88 % of the global mortality and disease burden from CVD occurs in low- and middle-income countries(1). For example, CVD causes over three million deaths each year in China, and at least 230 million Chinese are suffering from CVD. The origin of CVD is multifactorial and its pathological basis is atherosclerosis. Hypercholesterolaemia is recognised as a major and independent risk factor of atherosclerosis and CVD(Reference Lusis2). About 60–70 % of plasma cholesterol is present in the LDL fraction(Reference Halpern3). Plasma LDL-cholesterol (LDL-C) crosses the endothelial barrier and reaches the vascular subendothelial intima, whereby promoting monocyte/macrophage differentiation. Subsequently, monocyte-derived macrophages take up cholesterol through scavenger receptors and become lipid-laden foam cells(Reference Rader and Puré4). Steinberg et al. (Reference Steinberg, Parthasarathy and Carew5) showed that the cholesterol accumulating in macrophage-derived foam cells comes from circulating lipoproteins, mainly from the atherogenic LDL. Therefore, plasma lipids play a critical role in the initiation and progression of the atherosclerotic lesion(Reference Rader and Puré4), and it is essential to keep blood cholesterol levels in the appropriate range for the prevention of atherosclerosis and CVD. On the basis of a meta-analysis, a 1 % decrease in plasma cholesterol level can reduce the risk of CVD up to 3 %(Reference Brown, Stukovsky and Zhao6).

Hypolipidaemic drugs, particularly statins, are the preferred treatment for reducing blood cholesterol levels because of their efficacy and safety(Reference Armitage7). However, dietary modification is the mainstay of treatment of lipid abnormalities and can have a significant cholesterol-lowering effect(Reference Bruckert and Rosenbaum8).

Soyabean, as a traditional Chinese food, has been consumed for thousands of years in China due to its beneficial effects on health. Soya contains a variety of phytochemicals with special health functions, such as soya isoflavones, saponins, phytic acid, sterol and so on. Besides these, soya oligosaccharides (SOS) and soya oligopeptides (SOP) are two types of bioactive substances extracted from soyabean. SOS is the general term for soluble sugars in soyabean, and it is mainly composed of stachyose, raffinose and sucrose. SOP refers to peptides with molecular weights lower than 1000 Da, and it is derived from soya protein by enzyme hydrolysis. Some reports(Reference Sugano, Goto and Yamada9, Reference Anderson, Johnstone and Cook-Newell10) have suggested that both soya protein and SOP have effects on improving the lipid profile in hypercholesterolaemic subjects, whereas SOP is more effective than soya protein. Many studies found that oligosaccharides have cholesterol-lowering properties. However, little information is known about the effect of SOS on lipoprotein metabolism in high-cholesterol-fed animals, especially the combined effects of SOS and SOP on improving dyslipidaemia. It has been shown that probiotics, such as bifidobacteria and lactobacilli, have beneficial effects on cholesterol metabolism in vitro and in vivo (Reference Taylor and Williams11). Lan et al. (Reference Lan, Williams and Verstegena12) reported that SOS is a powerful prebiotic that can increase caecal bifidobacterium and lactobacilli populations in animals and human subjects. SOP is the necessary and suitable nutrient for the growth of intestinal bifidobacteria(Reference Bezkorovainy13).

In the present study, we aimed to determine whether SOS and/or SOP had significant effects on lipid metabolism in a rat model with hyperlipidaemia induced by a high-fat diet (HFD). Besides plasma lipid parameters, we examined vasoactive substances, antioxidant activity and faecal bile acid (FBA) excretion in order to explore the potential application of SOS and SOP in CVD prevention. The second aim of the study was to determine whether the combination of SOS and SOP had additive or synergistic effects on these parameters.

Materials and methods

Materials

SOS extracted from soyabean whey was provided by Shanghai Far-reaching Food Company Limited (Shanghai, China). The total sugar content was ≥ 70 %.

SOP was provided by Shandong Zhongshi Duqing Biotech Company Limited (Jinan, China). The peptide content was ≥ 80 %.

Animals, diets and experimental protocol

A total of sixty healthy male Sprague–Dawley rats were obtained from the Laboratory Animal Center of the Third Military Medical University, and the body weight (BW) ranged from 190 to 210 g. The animals were individually housed in metabolic cages in an environment-controlled room (20–23°C and 40–60 % of humidity) under a 12 h light–12 h dark cycle with free access to food and water. All animals were fed for 8 weeks, and BW was monitored biweekly throughout the study. Food intake was measured three times per week. The animal protocol was approved by the Third Military Medical University Institutional Animal Care Committee and conducted in accordance with the Third Military Medical University guidelines for the care and use of laboratory animals.

After a week of adaptation, sixty Sprague–Dawley rats were evenly divided into five groups according to diets as follows: regular diet (control), HFD, HFD enriched with 2 % of SOS (SOS), HFD enriched with 3 % of SOP (SOP) and HFD enriched with 2 % SOS and 3 % SOP (SOSP). The regular diet was composed of the following ingredients (g/100 g diet): bean cake, 20; wheat starch, 19; maize starch, 15; rice starch, 20; bran, 15; fishmeal, 5; bone meal, 3; yeast, 1; salt, 1; and cod liver oil, 1. The total protein/carbohydrate/fat content of the regular diet was approximately 18, 52 and 5 %, respectively. The ingredients of the HFD were as follows: 10 % lard, 2 % cholesterol, 1 % bile salt and 87 % regular diet. In the SOS group, HFD was supplemented with 2 % SOS by replacing 2 g of bran. In the SOP group, HFD was supplemented with 3 % SOP by replacing 3 g of fishmeal. In the SOSP group, HFD was supplemented with 2 % SOS and 3 % SOP by replacing 2 g of bran and 3 g of fishmeal, respectively. Diets of the HFD and experimental groups were iso-energetic and iso-nitrogenic. At the end of the experiment, after overnight fasting, rats were anaesthetised using pentobarbital sodium, and blood samples from the femoral vein were collected in tubes containing EDTA (1·4 g/l) and Trasylol (100 kU/l). Then, plasma was separated for subsequent biochemical analysis and stored at − 70°C. Immediately after killing the animal, liver tissues were dissected and stored at − 70°C for later antioxidant activity assay. Atherosclerosis index (AI) was calculated according to the Fridewald formula:

\begin{eqnarray} AI = LDL\hyphen C/HDL\hyphen C. \end{eqnarray}

All faeces were collected for three consecutive days at the end of the experiment. The collected faeces were stored at − 20°C for later determination of FBA content by phosphomolybdate colorimetry after 16 h of vacuum freeze-drying.

Biochemical analysis

Serum lipid profile analysis

Serum total cholesterol (TC), TAG, LDL-C and HDL-cholesterol (HDL-C) levels were determined by commercial enzymatic test kits (Biosino Bio-technology and Science Inc., Beijing, China) using an automatic biochemical analyser (Type AU2700; Olympus, Tokyo, Japan). ApoAI, apoB and apoE were determined by immunoturbidimetry using assay kits from Biosino Bio-technology and Science Inc. These procedures were fully automated and have been described in detail elsewhere(Reference Ikeda, Shibuya and Senba14).

Evaluation of antioxidant activities

Superoxide dismutase (SOD) activity in the serum and liver was determined by the xanthine oxidase method using a commercially available kit (SOD Detection Kit; Nanjing Jiancheng Bioengineering Institute, Nanjing, China)(Reference Oyanagui15). Malondialdehyde (MDA) in the serum and liver was measured by the thiobarbituric acid method(Reference Chen, Huang and Lin16) using a commercially available kit (MDA Detection Kit, Nanjing Jiancheng Bioengineering Institute). The assay was based on the ability of a chromogenic agent to react with MDA, yielding a stable chromophore with maximal absorbance at 532 nm.

Prostanoid release measurements

In order to measure the release of thromboxane A2 (TXA2) and prostacyclin (PGI2), direct RIA was carried out to determine the concentrations of thromboxane B2 and 6-ketoPGF1a, the stable degradation products of TXA2 and PGI2, respectively(Reference Bouaziz, Marie and Richert17). RIA kits were supplied by the General Hospital of PLA, Beijing, China.

Plasma nitric oxide assay

Plasma NO concentration was determined by colorimetric technique and nitrate reductase method using a Nitrate/Nitrite Colorimetric Assay kit (Nanjing Jiancheng Bioengineering Institute). This assay was based on the enzymatic conversion of nitrate to nitrite by nitrate reductase, and the concentration of NO was indirectly measured by determining both nitrate and nitrite levels in the plasma.

Determination of faecal bile acids

Total FBA was determined by spectrophotometry based on the colour reaction between phosphomolybdate and bile acid(Reference Hong and Meng18). Protein and pigment were precipitated from faeces by adding ethanol, and total bile acids were extracted by chloroform and methanol (2:1, v/v). Phosphomolybdate reagent (phosphomolybdate (2·5 g), glacial acetic acid (100 ml) and concentrated sulphuric acid (10 ml)) was used to form colour reaction, and then it was quantified by a 721 spectrophotometer (wavelength: 690 nm) (Shanghai Phenix Optical Scientific Instrument Company Limited, Shanghai, China).

Statistical analysis

All statistical calculations were carried out with Statistical Package of SPSS 13.0 (Release 13.01S China: Beijing Stats Data Mining Company Limited, Beijing, China; permanent license). Data were expressed as mean values and standard deviations. For data with normal distribution and homogeneity of variance, significance of difference (P < 0·05) between mean values was determined by ANOVA coupled with the least significant differences (LSD) test, whereas it was determined by the Dunnett-T3 test for data with heterogeneity of variance.

Results

Body weight and food intake

Table 1 shows that neither the pre-treatment BW of rats nor weight gains after the 8-week treatment were significantly different among the five groups. The mean daily food intake of rats in the HFD and experimental groups was higher than that of the control group during the 8-week experiment; however, the difference was not statistically significant.

Table 1 Effects of soya oligosaccharides (SOS) and soya oligopeptides (SOP) on body weight and food intake of rats*

(Mean values and standard deviations, n 12 per group)

HFD, high-fat diet; SOSP, HFD enriched with 2 % SOS and 3 % SOP.

* There were no significant difference between groups.

Lipid profile changes

Table 2 shows the effects of SOS and SOP on serum TC, TAG, LDL-C and HDL-C levels. Serum TC, TAG and LDL-C levels in the HFD group were significantly higher than those of the control group, whereas HDL-C level was significantly lower than that of the control group (P < 0·05). The addition of SOS and SOP alone or together with HFD resulted in significantly decreased serum TC, TAG and LDL-C levels, whereas HDL-C level was significantly increased compared with the HFD group (P < 0·05). In particular, the combination of SOS and SOP was significantly more effective than SOS and SOP alone in altering TC, LDL-C and HDL-C levels (P < 0·05).

Table 2 Effects of soya oligosaccharides (SOS) and soya oligopeptides (SOP) on lipid profile of rats

(Mean values and standard deviations, n 12 per group)

TC, total cholesterol; LDL-C, LDL-cholesterol; HDL-C, HDL-cholesterol; HFD, high-fat diet; SOSP, HFD enriched with 2 % SOS and 3 % SOP.

* Mean values were significantly different from those of the control group (P < 0·05).

† Mean values were significantly different from those of the HFD group (P < 0·05).

‡ Mean values were significantly different from those of the SOS group (P < 0·05).

§ Mean values were significantly different from those of the SOP group (P < 0·05).

Fig. 1 shows the change of AI value of each group. AI value of the HFD group was significantly higher than that of the control group, whereas the value of the three experimental groups was significantly lower than that of the HFD group (P < 0·05). There was no statistically significant difference among the control and experimental groups.

Fig. 1 Changes of atherosclerosis index (AI). Values are means and standard deviations represented by vertical bars. * Mean value was significantly different from that of the control group (P < 0·05). † Mean values were significantly different from that of the high-fat diet (HFD) group (P < 0·05). LDL-C, LDL-cholesterol; HDL-C, HDL-cholesterol; SOP, soya oligopeptides; SOS, soya oligosaccharides; SOSP, HFD enriched with 2 % SOS and 3 % SOP.

Effects of soya oligosaccharides and soya oligopeptides on plasma apo levels

Table 3 shows the effects of SOS and SOP on plasma apo levels in rats. Plasma apoAI level in the HFD group was significantly lower, whereas apoB and apoE levels were significantly higher than those of the control group (P < 0·05). ApoAI levels of the three experimental groups were significantly increased compared with the HFD and control groups (P < 0·05), and the most significant effect was observed in the SOSP group (P < 0·05). ApoB levels of the SOP and SOSP groups were significantly decreased compared with those of the other three groups, and apoB level of the SOS group was significantly decreased compared with that of the HFD group (P < 0·05). ApoE level of the three experimental groups was significantly lower than that of the HFD group (P < 0·05), and the values were not statistically different from the control except for the SOS group. Comparisons within the three experimental groups revealed that the significant difference was only obtained between the SOS and SOSP groups (P < 0·05).

Table 3 Effects of soya oligosaccharides (SOS) and soya oligopeptides (SOP) on plasma apo levels of rats

(Mean values and standard deviations, n 12 per group)

HFD, high-fat diet; SOSP, HFD enriched with 2 % SOS and 3 % SOP.

* Mean values were significantly different from those of the control group (P < 0·05).

† Mean values were significantly different from those of the HFD group (P < 0·05).

‡ Mean values were significantly different from those of the SOS group (P < 0·05).

§ Mean values were significantly different from those of the SOP group (P < 0·05).

In terms of the apoB:apoAI ratio, it was significantly increased in the HFD group compared with that of the control group, whereas it was significantly decreased in the three experimental groups compared with that in both HFD and control groups (P < 0·05). Furthermore, the apoB:apoAI ratio of the three experimental groups was in an order as follows: SOSP group < SOP group < SOS group (P < 0·05).

Effects of soya oligosaccharides and soya oligopeptides on vasoactive substances

Table 4 shows the effects of SOS and SOP on plasma vasoactive substances. Plasma PGI2 and NO levels of the HFD group were significantly lower than those of the control group, whereas TXA2 and the TXA2:PGI2 ratio of the HFD group were significantly higher than those of the control group (P < 0·05). Plasma PGI2 and NO levels of the three experimental groups were significantly higher than those of the HFD group, whereas TXA2 and the TXA2:PGI2 ratio of the three experimental groups were significantly lower than those of the HFD group, but higher than those of the control group (P < 0·05). Compared within the three experimental groups, the SOSP group was more effective in reducing TXA2 and the TXA2:PGI2 ratio, as well as increasing NO than the SOS or SOP group (P < 0·05).

Table 4 Effects of soya oligosaccharides (SOS) and soya oligopeptides (SOP) on vasoactive substances of rats

(Mean values and standard deviations, n 12 per group)

TXA2, thromboxane A2; PGI2, prostacyclin; NO, nitric oxide; HFD, high-fat diet; SOSP, HFD enriched with 2 % SOS and 3 % SOP.

* Mean values were significantly different from those of the control group (P < 0·05).

† Mean values were significantly different from those of the HFD group (P < 0·05).

‡ Mean values were significantly different from those of the SOS group (P < 0·05).

§ Mean values were significantly different from those of the SOP group (P < 0·05).

Superoxide dismutase activity and malondialdehyde content in livers and serum

Table 5 shows that SOD activity in the serum, as well as MDA contents in the liver and serum of the HFD group were significantly higher than those of the control group (P < 0·05), whereas SOD activity in the liver of the HFD group was not statistically different from that of the control group. SOD activity in the liver of the three experimental groups was significantly lower than that of the control group (P < 0·05). SOD activity in serum and the MDA content in the liver and serum of the three experimental groups were significantly higher than those of the control group, but lower than those of the HFD group (P < 0·05), except for the liver MDA content of the SOSP group, which was not significantly different from that of the control group. Among the three experimental groups, the MDA content in the liver of the SOSP group was significantly lower than that of the SOS group (P < 0·05), whereas no significance was obtained from the other experimental groups.

Table 5 Effects of soya oligosaccharides (SOS) and soya oligopeptides (SOP) on superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in liver and serum of rats

(Mean values and standard deviations, n 12 per group)

HFD, high-fat diet; SOSP, HFD enriched with 2 % SOS and 3 % SOP.

* Mean values were significantly different from those of the control group (P < 0·05).

† Mean values were significantly different from those of the HFD group (P < 0·05).

‡ Mean values were significantly different from those of the SOS group (P < 0·05).

Dry faecal weight and bile acid excretion

Table 6 shows the FBA levels of rats. Dry faecal weight of the HFD group and the three experimental groups were higher than that of the control group. However, there were no significant differences among any groups (P>0·05). FBA of the three experimental groups was significantly higher than that of the HFD and control groups, and FBA of the HFD group was significantly higher than that of the control group (P < 0·05). Compared with the three experimental groups, FBA content of the SOSP group was significantly higher than that of the other two experimental groups (P < 0·05).

Table 6 Effects of soya oligosaccharides (SOS) and soya oligopeptides (SOP) on faecal dry weight and bile acids of rats

(Mean values and standard deviations, n 12 per group)

HFD, high-fat diet; SOSP, HFD enriched with 2 % SOS and 3 % SOP.

* Mean values were significantly different from those of the control group (P < 0·05).

† Mean values were significantly different from those of the HFD group (P < 0·05).

‡ Mean values were significantly different from those of the SOS group (P < 0·05).

§ Mean values were significantly different from those of the SOP group (P < 0·05).

Discussion

In the present study, we did not observe significant differences of the mean daily food intake and BW gains among five groups, indicating that the consumption of diet by rats was not altered by high fat, SOS and SOP, and thus the BW gains were not significantly different among the five groups.

The reduction of TC or LDL-C in plasma has been reported to reduce the risk of CVD. Numerous studies in human subjects and animals have shown the hypocholesterolaemic effect of soya protein(Reference Anderson, Johnstone and Cook-Newell10, Reference Tonstad, Smerud and Høie19, Reference Teixeira, Potter and Weigel20). Some studies reported that the effect of soya protein on lipid metabolism is attributed to its component of peptides(Reference Hori, Wang and Chan21, Reference Duranti, Lovati and Dani22). In the present study, we observed that serum TC and TAG of the SOP group were decreased by 34·7 and 40·2 % compared with the HFD group, respectively. These results are consistent with findings of Duranti et al. (Reference Duranti, Lovati and Dani22), who showed that plasma TC and TAG of rats fed a hypercholesterolaemic diet were reduced by 36 and 34 %, respectively, by treatment with 20 mg/kg BW per d of soya 7S globulin-α’ subunit for 28 d. By suppressing cholesterol micelle solubility, Zhong et al. (Reference Zhong, Zhang and Ma23) showed that SOP interferes with the micellar solubilisation of cholesterol, resulting in decreased intestinal cholesterol absorption and ensuing serum cholesterol reduction.

Chen et al. (Reference Chen, Liu and Zhu24) reported that SOS effectively reduces serum TC and TAG levels, thus improving the HFD-induced abnormal blood lipid level in rats. Similarly, the present study showed that SOS reduced serum TC and TAG concentrations of hyperlipidaemic rats by 37·7 and 50·4 %, respectively. With a powerful prebiotic ability, SOS is fermented in the colon by bifidobacterium, yielding large amounts of SCFA, such as butyrate and propionate(Reference Bang, Chio and Kim25). It has been hypothesised that SCFA, in particular propionate and butyrate, are able to influence lipid metabolism(Reference Bornet, Alamowitch, Slama, Philips, Weldlock and Williams26). Butyrate can inhibit hepatic cholesterol synthesis, while propionate may inhibit the synthesis of fatty acids in the liver, thereby reducing the rates of TAG secretion(Reference Trautwein, Rieckhoff and Erbersdobler27). Therefore, the present data suggested that the improved lipid metabolism observed in the SOS group was partly caused by an alteration in the absorption and/or synthesis of cholesterol through SCFA. In the present study, we observed that the combined treatment of SOS and SOP reduced TC and TAG levels by 10·3, 14·4 and 4·4 %, 20·6 % compared with SOS and SOP alone, respectively, suggesting that the combination of SOS and SOP was more effective in improving dyslipidaemia, thus reducing the risk of atherosclerotic CVD.

There is increasing evidence that both reducing LDL-C and raising HDL-C can result in significant cardiovascular benefit(Reference Sirtori and Fumagalli28). In the present study, LDL-C was reduced by 66·9 % in the SOP group and by 59·4 % in the SOS group, whereas HDL-C was increased by 112·5 and 119·2 %, respectively. These results suggested that these effects of SOS and SOP on LDL-C and HDL-C were beneficial for atherosclerotic CVD. Some studies confirmed the reduction in plasma TC and LDL-C without significant effect on HDL-C by soya protein(Reference Tonstad, Smerud and Høie19, Reference Hermansen, Sondergaard and Høie29). Zhao et al. (Reference Zhao, Wang and Wu30) observed that SOP reduces serum TC and TAG levels of hyperlipidaemic rats, whereas HDL-C is not significantly altered. However, we observed a significant increase of plasma HDL-C in the SOP group. The precise reason for this inconsistency with other reports remains unclear. It may result from the SOP used in the present study, which was different from other studies, especially the molecular weight of SOP. It has been proposed that the regulation of cholesterol homoeostasis by SOP depends on the activation of LDL receptors and LDL degradation in the liver(Reference Erdmann, Cheung and Schröder31). Lovati et al. (Reference Lovati, Manzoni and Gianazza32) demonstrated that peptides derived from soya 7S globulin-α’ subunit are able to up-regulate LDL receptors’ activity, thus resulting in receptor-mediated LDL catabolism(Reference Manzoni, Duranti and Eberini33). However, 7S lacking the α’-subunit has no effect on the up-regulation of LDL receptors, indicating that soya peptides with hypocholesterolaemic activity are involved in the regulation of LDL receptors. The observed decrease in AI values suggested that SOS and SOP, especially their combination, were auxiliary in decreasing the risk of atherosclerosis and CVD.

High apoB, the structural protein of LDL, and a high apoB:apoAI ratio are strongly related to the increased atherosclerotic cardiovascular risk. In contrast, high apoAI, mainly representing HDL and being crucial in transferring cholesterol from tissues to the liver, is inversely related to atherogenic risk(Reference Walldius, Jungner and Aastveit34). In the present study, we observed a significantly higher apoB and apoB:apoAI ratio, exhibiting an atherogenic lipid profile as well as a significantly lower apoAI in HFD-fed rats. The present data also showed a significant reduction of apoB by 43·3 % and an increase of apoAI by 197·8 % in SOS-fed rats. In a clinical study, Teixeira et al. (Reference Teixeira, Potter and Weigel20) observed that soya protein effectively reduces plasma apoB, whereas the change in apoAI is not significant. However, in the present study, SOP not only reduced apoB but also increased apoAI effectively. Moreover, the combination of SOP and SOS showed a more marked effect on reducing the apoB:apoAI ratio and increasing apoAI than SOP and SOS alone. These data indicated that the combination of SOP and SOS could be more effective in reducing serum lipid risk factors for CVD than SOP or SOS alone.

Endothelial dysfunction plays an important role in the early development of atherosclerosis(Reference Sitia, Tomasoni and Atzeni35). PGI2 and NO are anti-atherogenic because of their vasodilating effects, whereas TXA2 is a potent vasoconstrictor(Reference Praticò, Cyrus and Li36–Reference Var, Yildirim and Onur38). In the present study, we observed that HFD reduced the release of PGI2 and NO, and increased the TXA2 level, suggesting that endothelium-dependent vasorelaxation was attenuated by hyperlipidaemia(Reference Dusting, Hyland and Hickey39), and the effect of hypercholesterolaemia on endothelial cells leads to the inhibition of NO synthesis(Reference Sitia, Tomasoni and Atzeni35). In vitro study has shown that SOP increases the release of PGI2 and NO, but it does not affect TXA2(Reference Ringseis, Matthes and Lehmann40). The present study showed that SOP not only increased the release of PGI2 and NO by 67·9 and 48·9 %, respectively, but also decreased TXA2 by 53·6 % compared with the HFD group. SOS exerted similar effects. Furthermore, the combination of SOS and SOP was more effective in reducing TXA2 and the TXA2:PGI2 ratio, as well as increasing NO than SOS or SOP alone, thus improving endothelial dysfunction and cardiovascular health.

It has been shown that oxidative stress contributes to endothelial dysfunction and atherosclerosis(Reference Lusis2). However, dietary antioxidants may protect against oxidative events in the body, and the antioxidant activity reduces CVD by preventing LDL oxidation(Reference Sitia, Tomasoni and Atzeni35, Reference Mesa, Silván and Olza41). In the present study, oxidative stress occurred in HFD-fed rats with elevated liver and serum levels of MDA, the ultimate product of lipid peroxidation. The antioxidant defence system, such as SOD, is important to protect the body from oxidative damage(Reference Mahfouz and Kummerow42). Many experimental results showed that HFD or hyperlipidaemia can reduce SOD activity in rats(Reference Watanabe and Fukui43). Surprisingly, in the present study, the serum SOD activity of the HFD group was not reduced, and it was higher than that of the control and experimental groups. The present findings are consistent with those of Arafa et al. (Reference Arafa44), who showed that a HFD does not decrease the liver SOD activity, which is higher than that of normal diet-fed rats and curcumin-treated rats. We supposed that the result was due to the high level of MDA, which resulted in the increase of SOD activity for maintaining the balance between oxidative stress and antioxidant defences.

Gibbs et al. (Reference Gibbs, Zougman and Masse45) reported that the antioxidant activity of soya protein is associated with the fractions 11S or glycinin as the precursor of antioxidant peptides. In another study, it has been shown that the ingestion of soya peptide in rats reduces paraquat-induced oxidative stress by preventing the elevation of the concentration of serum thiobarbituric acid-reactive substances, thus effectively preventing LDL oxidation(Reference Takenaka, Annaka and Kimura46). The present data show that SOP decreases MDA by 32·8 and 51·6 % in the liver and serum, respectively, thus reducing oxidative stress. Chen et al. (Reference Chen, Muramoto and Yamauchi47) found that peptides with a Pro-His-His sequence demonstrate the greatest antioxidant activity among all tested peptides, and their activity is decreased by removing a His residue from the C-terminus. Chen et al. (Reference Chen, Liu and Zhu24) reported that the activities of antioxidant enzymes in the liver and serum, such as catalase and SOD, are significantly elevated when SOS is orally administered to rats for 45 d. The present data show that SOS could reduce oxidative stress by increasing the serum SOD activity and reducing the MDA content in SOS-treated rats, suggesting that SOS protected blood vessels and endothelial cells from oxidative damage by inhibiting LDL oxidation, thus protecting against atherosclerosis.

Bile acids are critical in maintaining cholesterol homoeostasis. Binding of bile acids and increasing their faecal excretion have been hypothesised as an effective way to reduce cholesterol by dietary factors(Reference Anderson, Siesel, Furda and Brine48). Iwami et al. (Reference Iwami, Sakakibara and Ibuki49) reported that SOP binds bile acids, thereby enhancing bile acid and faecal steroid excretion, which may contribute to the hypocholesterolaemic activity. In the present study, SOP-fed rats demonstrated significantly greater FBA excretion than those fed with normal diets or HFD. Greater FBA excretion leads to the increased bile acid production from cholesterol, thus reducing serum cholesterol concentrations. Choi et al. (Reference Choi, Adachi and Utsumi50) identified the bile acid-binding region in SOP and found that the glycinin-derived peptide VAWWMY is responsible for this bile acid-binding ability. Therefore, the present data support the hypothesis that the absorption of cholesterol and bile acids could be further decreased by SOP, and the increased bile acid excretion is an important contributor to the hypocholesterolaemic effect of SOP. SOS-fed rats also showed significantly higher excretion of FBA compared with the control and HFD groups. A 30 d study in human subjects(Reference Bang, Chio and Kim25) showed that SOS intake of 3 g/d significantly increases faecal bifidobacteria counts, SCFA concentrations and lipid output in women. The cholesterol-lowering effect of bifidobacteria is attributed to increasing deconjugation of bile acids, production of SCFA and cholesterol binding in the intestinal lumen(Reference Taylor and Williams11). Therefore, we suggested that increasing FBA excretion associated with promotion of intestinal bifidobacteria proliferation and the production of SCFA by SOS is also important for blood cholesterol-lowering and cardioprotective effects. In the present study, we observed that a combination of SOS and SOP was more effective in increasing FBA excretion than SOS and SOP alone. SOS is a powerful prebiotic oligosaccharide, while SOP is also suitable to sustain growth of intestinal bifidobacteria. Therefore, the increasing FBA excretion by the combination of SOS and SOP could be ascribed to an additive or synergistic effect.

In summary, the present study shows that ingestion of 2 % SOS or 3 % SOP influence lipid metabolism, release of vasoactive substances as well as antioxidant activity and increase FBA excretion. In particular, the combination of 2 % SOS and 3 % SOP is more effective than SOS or SOP alone, especially in improving the lipid profile and increasing FBA excretion. These results indicate that SOS and SOP have potential value in CVD prevention. Further studies should focus on clarifying the mechanisms of action of the lipid effects of SOS and SOP.

Acknowledgements

The authors thank the Department of Nutrition and Food Hygiene, Third Military Medical University, for laboratory and technical support of the present study. They are also grateful to Dr Fang Chen and Junhua Peng for their valuable discussions on the methods of bile acid measurement. This work was supported by the Sci & Tech Research Project Foundation of Scientific Committee of Chongqing (200300168). S. X., J. Z. and X. M. designed the research; S. X. conducted the research; Y. Z., K. S. and Y. S. analysed the data; and S. X. and J. Z. wrote the manuscript.

References

1World Health Organization (2008) The Global Burden of Disease: 2004 Update. Geneva, Switzerland: WHO. http://www.who.int/healthinfo/global_burden_disease/GBD_report_2004update_full.pdf (accessed September 2010).Google Scholar
2Lusis, AJ (2000) Atherosclerosis. Nature 407, 233–241.CrossRefGoogle ScholarPubMed
3Halpern, MJ (1995) Lipids and atherosclerosis. Mol Aspects Med 16, 509–710.CrossRefGoogle ScholarPubMed
4Rader, DJ & Puré, E (2005) Lipoproteins, macrophage function, and atherosclerosis: beyond the foam cell? Cell Metab 1, 223–230.CrossRefGoogle ScholarPubMed
5Steinberg, D, Parthasarathy, S, Carew, TE, et al. (1989) Beyond cholesterol. Modifications of low-density lipoprotein that increase its atherogenicity. N Engl J Med 320, 915–924.Google ScholarPubMed
6Brown, BG, Stukovsky, KH & Zhao, XQ (2006) Simultaneous low-density lipoprotein-C lowering and high-density lipoprotein-C elevation for optimum cardiovascular disease prevention with various drug classes, and their combinations: a meta-analysis of 23 randomized lipid trials. Curr Opin Lipidol 17, 631–636.CrossRefGoogle ScholarPubMed
7Armitage, J (2007) The safety of statins in clinical practice. Lancet 370, 1781–1790.CrossRefGoogle ScholarPubMed
8Bruckert, E & Rosenbaum, D (2011) Lowering LDL-cholesterol through diet: potential role in the statin era. Curr Opin Lipidol 22, 43–48.CrossRefGoogle ScholarPubMed
9Sugano, M, Goto, S, Yamada, Y, et al. (1990) Cholesterol-lowering activity of various undigested fractions of soybean protein in rats. J Nutr 120, 977–985.CrossRefGoogle ScholarPubMed
10Anderson, JW, Johnstone, BM & Cook-Newell, ME (1995) A meta-analysis of the effects of soy protein intake on serum lipids. N Engl J Med 333, 276–282.CrossRefGoogle ScholarPubMed
11Taylor, GR & Williams, CM (1998) Effects of probiotics and prebiotics on blood lipids. Br J Nutr 80, S225–S230.CrossRefGoogle ScholarPubMed
12Lan, Y, Williams, BA, Verstegena, MWA, et al. (2007) Soy oligosaccharides in vitro fermentation characteristics and its effect on caecal microorganisms of young broiler chickens. Anim Feed Sci Technol 133, 286–297.CrossRefGoogle Scholar
13Bezkorovainy, A (2001) Probiotics: determinants of survival and growth in the gut. Am J Clin Nutr 73, 399S–405S.CrossRefGoogle ScholarPubMed
14Ikeda, T, Shibuya, Y, Senba, U, et al. (1991) Automated immunoturbidimetric analysis of six plasma apoliproteins: correlation with radial immunodiffusion assays. J Clin Lab Anal 5, 90–95.CrossRefGoogle Scholar
15Oyanagui, Y (1984) Reevaluation of assay methods and establishment of kit for superoxide dismutase activity. Anal Biochem 142, 290–296.CrossRefGoogle ScholarPubMed
16Chen, CY, Huang, YL & Lin, TH (1998) Association between oxidative stress and cytokine production in nickel-treated rats. Arch Biochem Biophys 356, 127–132.CrossRefGoogle ScholarPubMed
17Bouaziz, A, Marie, M, Richert, A, et al. (1998) Direct physical factors and PGI2 and TXA2 secretions by a human endothelial cell line: in vitro investigation of pressure and shear stress applied independently or in synergy. Thromb Res 90, 279–289.CrossRefGoogle ScholarPubMed
18Hong, NK & Meng, CW (1983) Determination of total bile acids in bile by phosphomolybdate colorimetry. Shanghai Med J 6, 35–37.Google Scholar
19Tonstad, S, Smerud, K & Høie, L (2002) A comparison of the effects of 2 doses of soy protein or casein on serum lipids, serum lipoproteins, and plasma total homocysteine in hypercholesterolemic subjects. Am J Clin Nutr 76, 78–84.CrossRefGoogle ScholarPubMed
20Teixeira, SR, Potter, SM, Weigel, R, et al. (2000) Effects of feeding 4 levels of soy protein for 3 and 6 wk on blood lipids and apolipoproteins in moderately hypercholesterolemic men. Am J Clin Nutr 71, 1077–1084.CrossRefGoogle ScholarPubMed
21Hori, G, Wang, MF, Chan, YC, et al. (2001) Soy protein hydrolyzate with bound phospholipids reduces serum cholesterol levels in hypercholesterolemic adult male volunteers. Biosci Biotechnol Biochem 65, 72–78.CrossRefGoogle ScholarPubMed
22Duranti, M, Lovati, MR, Dani, V, et al. (2004) The alpha’ subunit from soybean 7S globulin lowers plasma lipids and upregulates liver beta-VLDL receptors in rats fed a hypercholesterolemic diet. J Nutr 134, 1334–1339.CrossRefGoogle ScholarPubMed
23Zhong, F, Zhang, XM, Ma, JG, et al. (2007) Fractionation and identification of a novel hypocholesterolemic peptide derived from soy protein Alcalase hydrolysates. Food Res Int 40, 756–762.CrossRefGoogle Scholar
24Chen, H, Liu, LJ, Zhu, JJ, et al. (2010) Effect of soybean oligosaccharides on blood lipid, glucose levels and antioxidant enzymes activity in high fat rats. Food Chem 119, 1633–1636.CrossRefGoogle Scholar
25Bang, MH, Chio, OS & Kim, WK (2007) Soy oligosaccharide increases fecal bifidobacteria counts, short-chain fatty acids, and fecal lipid concentrations in young Korean women. J Med Food 10, 366–370.CrossRefGoogle Scholar
26Bornet, F, Alamowitch, C & Slama, G (1994) Short chain fatty acids and metabolic effects in humans. In Gum and Stabilisers for Food Industry, 7th ed., pp. 217–229 [Philips, GO, Weldlock, DJ and Williams, TE, editors]. Oxford: Oxford University Press.Google Scholar
27Trautwein, EA, Rieckhoff, D & Erbersdobler, HF (1998) Dietary inulin lowers plasma cholesterol and triacylglycerol and alters biliary bile acid profile in hamsters. J Nutr 128, 1937–1943.CrossRefGoogle ScholarPubMed
28Sirtori, CR & Fumagalli, R (2006) LDL-cholesterol lowering or HDL-cholesterol raising for cardiovascular prevention. A lesson from cholesterol turnover studies and others. Atherosclerosis 186, 1–11.CrossRefGoogle ScholarPubMed
29Hermansen, K, Sondergaard, M, Høie, L, et al. (2001) Beneficial effects of a soy-based dietary supplement on lipid levels and cardiovascular risk markers in type 2 diabetic subjects. Diabetes Care 24, 228–233.CrossRefGoogle ScholarPubMed
30Zhao, XJ, Wang, XX, Wu, BX, et al. (2002) Regulation effects of soy active peptides on lipid parameters of rats fed a high fat diet. Chin J Health Lab Technol 12, 421–422.Google Scholar
31Erdmann, K, Cheung, BW & Schröder, H (2008) The possible roles of food-derived bioactive peptides in reducing the risk of cardiovascular disease. J Nutr Biochem 19, 643–654.CrossRefGoogle ScholarPubMed
32Lovati, MR, Manzoni, C, Gianazza, E, et al. (2000) Soy protein peptides regulate cholesterol homeostasis in Hep G2 cells. J Nutr 130, 2543–2549.Google ScholarPubMed
33Manzoni, C, Duranti, M, Eberini, I, et al. (2003) Subcellular localization of soybean 7S globulin in HepG2 cells and LDL receptor up-regulation by its alphaV constituent subunit. J Nutr 133, 2149–2155.CrossRefGoogle Scholar
34Walldius, G, Jungner, I, Aastveit, AH, et al. (2004) The apoB/apoA-I ratio is better than the cholesterol ratios to estimate the balance between plasma proatherogenic and antiatherogenic lipoproteins and to predict coronary risk. Clin Chem Lab Med 42, 1355–1363.CrossRefGoogle Scholar
35Sitia, S, Tomasoni, L, Atzeni, F, et al. (2010) From endothelial dysfunction to atherosclerosis. Autoimmun Rev 9, 830–834.CrossRefGoogle ScholarPubMed
36Praticò, D, Cyrus, T, Li, H, et al. (2000) Endogenous biosynthesis of thromboxane and prostacyclin in 2 distinct murine models of atherosclerosis. Blood 96, 3823–3826.CrossRefGoogle ScholarPubMed
37Naseem, KM (2005) The role of nitric oxide in cardiovascular diseases. Mol Aspects Med 26, 33–65.CrossRefGoogle ScholarPubMed
38Var, A, Yildirim, Y, Onur, E, et al. (2003) Endothelial dysfunction in preeclampsia. Increased homocysteine and decreased nitric oxide levels. Gynecol Obstet Invest 56, 221–224.CrossRefGoogle ScholarPubMed
39Dusting, GJ, Hyland, R, Hickey, H, et al. (1995) Angiotensin-converting enzyme inhibitors reduce neointimal thickening and maintain endothelial nitric oxide function in rabbit carotid arteries. Am J Cardiol 76, 24E–27E.CrossRefGoogle ScholarPubMed
40Ringseis, R, Matthes, B, Lehmann, V, et al. (2005) Peptides and hydrolysates from casein and soy protein modulate the release of vasoactive substances from human aortic endothelial cells. Biochim Biophys Acta 1721, 89–97.CrossRefGoogle ScholarPubMed
41Mesa, MD, Silván, JM, Olza, J, et al. (2008) Antioxidant properties of soy protein-fructooligosaccharide glycation systems and its hydrolyzates. Food Res Int 41, 606–615.CrossRefGoogle Scholar
42Mahfouz, MM & Kummerow, FA (2000) Cholesterol-rich diets have different effects on lipid peroxidation, cholesterol oxides, and antioxidant enzymes in rats and rabbits. J Nutr Biochem 11, 293–302.CrossRefGoogle ScholarPubMed
43Watanabe, S & Fukui, T (2000) Suppressive effect of curcumin on trichloroethylene-induced oxidative stress. J Nutr Sci Vitaminol (Tokyo) 46, 230–234.CrossRefGoogle ScholarPubMed
44Arafa, HM (2005) Curcumin attenuates diet-induced hypercholesterolemia in rats. Med Sci Monit 11, BR228–BR234.Google ScholarPubMed
45Gibbs, BF, Zougman, A, Masse, R, et al. (2004) Production and characterization of bioactive peptides from soy hydrolysate and soy-fermented food. Food Res Int 37, 123–131.CrossRefGoogle Scholar
46Takenaka, A, Annaka, H, Kimura, Y, et al. (2003) Reduction of paraquat-induced oxidative stress in rats by dietary soy peptide. Biosci Biotechnol Biochem 67, 278–283.CrossRefGoogle ScholarPubMed
47Chen, HM, Muramoto, K, Yamauchi, F, et al. (1996) Antioxidant activity of designed peptides based on the antioxidative peptide isolated from digests of a soybean protein. J Agric Food Chem 44, 2619–2623.CrossRefGoogle Scholar
48Anderson, JW & Siesel, AE (1990) Hypocholesterolemic effects of oat products. In New Developments In Dietary Fiber: Physiological, Physiochemical, and Analytical Aspects, pp. 17–36 [Furda, I and Brine, CJ, editors]. New York, NY: Plenum Press.CrossRefGoogle Scholar
49Iwami, K, Sakakibara, K & Ibuki, F (1986) Involvement of post-digestion hydrophobic peptides in plasma cholesterol-lowering effect of dietary plant protein. Agric Biol Chem 50, 1217–1222.Google Scholar
50Choi, SK, Adachi, M & Utsumi, S (2002) Identification of the bile acid-binding region in the soy glycinin A1aB1b subunit. Biosci Biotechnol Biochem 66, 2395–2401.CrossRefGoogle ScholarPubMed
Figure 0

Table 1 Effects of soya oligosaccharides (SOS) and soya oligopeptides (SOP) on body weight and food intake of rats*(Mean values and standard deviations, n 12 per group)

Figure 1

Table 2 Effects of soya oligosaccharides (SOS) and soya oligopeptides (SOP) on lipid profile of rats(Mean values and standard deviations, n 12 per group)

Figure 2

Fig. 1 Changes of atherosclerosis index (AI). Values are means and standard deviations represented by vertical bars. * Mean value was significantly different from that of the control group (P < 0·05). † Mean values were significantly different from that of the high-fat diet (HFD) group (P < 0·05). LDL-C, LDL-cholesterol; HDL-C, HDL-cholesterol; SOP, soya oligopeptides; SOS, soya oligosaccharides; SOSP, HFD enriched with 2 % SOS and 3 % SOP.

Figure 3

Table 3 Effects of soya oligosaccharides (SOS) and soya oligopeptides (SOP) on plasma apo levels of rats(Mean values and standard deviations, n 12 per group)

Figure 4

Table 4 Effects of soya oligosaccharides (SOS) and soya oligopeptides (SOP) on vasoactive substances of rats(Mean values and standard deviations, n 12 per group)

Figure 5

Table 5 Effects of soya oligosaccharides (SOS) and soya oligopeptides (SOP) on superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in liver and serum of rats(Mean values and standard deviations, n 12 per group)

Figure 6

Table 6 Effects of soya oligosaccharides (SOS) and soya oligopeptides (SOP) on faecal dry weight and bile acids of rats(Mean values and standard deviations, n 12 per group)