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Are the number of nucleolar precursor body and size of pronuclear correlated with embryo development and ploidy status in 1PN zygotes?: an analysis through the time-lapse monitoring and pre-implantation genetic testing

Published online by Cambridge University Press:  13 December 2024

Shun Xiong Open the ORCID record for Shun Xiong [Opens in a new window] ,
Jiang Wang Open the ORCID record for Jiang Wang [Opens in a new window] ,
Yang Gao ,
Lihong Wu ,
Xiangwei Hao ,
Jia hong Zhu Open the ORCID record for Jia hong Zhu [Opens in a new window] ,
Keya Tong ,
Guoning Huang Open the ORCID record for Guoning Huang [Opens in a new window] ,
Jun xia Liu  and
Wei Han
Shun Xiong
Affiliation:
Chongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine Center, Women and Children’s Hospital of Chongqing Medical University, Chongqing, China Chongqing Clinical Research Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, China
Jiang Wang
Affiliation:
Chongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine Center, Women and Children’s Hospital of Chongqing Medical University, Chongqing, China Chongqing Clinical Research Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, China
Yang Gao
Affiliation:
Chongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine Center, Women and Children’s Hospital of Chongqing Medical University, Chongqing, China Chongqing Clinical Research Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, China
Lihong Wu
Affiliation:
Chongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine Center, Women and Children’s Hospital of Chongqing Medical University, Chongqing, China Chongqing Clinical Research Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, China
Xiangwei Hao
Affiliation:
Chongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine Center, Women and Children’s Hospital of Chongqing Medical University, Chongqing, China Chongqing Clinical Research Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, China
Jia hong Zhu
Affiliation:
Chongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine Center, Women and Children’s Hospital of Chongqing Medical University, Chongqing, China Chongqing Clinical Research Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, China
Keya Tong
Affiliation:
Chongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine Center, Women and Children’s Hospital of Chongqing Medical University, Chongqing, China Chongqing Clinical Research Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, China
Guoning Huang
Affiliation:
Chongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine Center, Women and Children’s Hospital of Chongqing Medical University, Chongqing, China Chongqing Clinical Research Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, China
Jun xia Liu*
Affiliation:
Chongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine Center, Women and Children’s Hospital of Chongqing Medical University, Chongqing, China Chongqing Clinical Research Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, China
Wei Han*
Affiliation:
Chongqing Key Laboratory of Human Embryo Engineering, Center for Reproductive Medicine Center, Women and Children’s Hospital of Chongqing Medical University, Chongqing, China Chongqing Clinical Research Center for Reproductive Medicine, Chongqing Health Center for Women and Children, Chongqing, China
*
Corresponding authors: Junxia Liu; Email: [email protected]; Wei Han; Email: [email protected]
Corresponding authors: Junxia Liu; Email: [email protected]; Wei Han; Email: [email protected]
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Abstract

This study aimed to demonstrate the utilization value of 1PN embryos. The 1PN zygotes collected from December 2021 to September 2022 were included in this study. The embryo development, the pronuclear characteristics, and the genetic constitutions were investigated. The overall blastocyst formation and good-quality blastocyst rates in 1PN zygotes were 22.94 and 16.24%, significantly lower than those of 2PN zygotes (63.25 and 50.23%, respectively, P = 0.000). The pronuclear characteristics were found to be correlated with the developmental potential. When comparing 1PN zygotes that developed into blastocysts to those that arrested, the former exhibited a significantly larger area (749.49 ± 142.77 vs. 634.00 ± 119.05, P = 0.000), a longer diameter of pronuclear (29.81 ± 3.08 vs. 27.30 ± 3.00, P = 0.000), and a greater number of nucleolar precursor body (NPB) (11.56 ± 3.84 vs. 7.19 ± 2.73, P = 0.000). Among the tested embryos, the diploidy euploidy rate was significantly higher in blastocysts in comparison with the arrested embryos (66.67 vs. 11.76%, P = 0.000), which was also significantly higher in IVF-1PN blastocysts than in ICSI-1PN blastocysts (75.44 vs. 25.00%, P = 0.001). However, the pronuclear characteristics were not found to be linked to the chromosomal ploidy once they formed blastocysts.

In summary, while the developmental potential of 1PN zygotes is reduced, our study shows that, in addition to the reported pronuclear area and diameter, the number of NPB is also associated with their developmental potential. The 1PN blastocysts exhibit a high diploidy euploidy rate, are recommend to be clinically used post genetic testing, especially for patients who do not have other 2PN embryos available.

Keywords

chromosomal ploidydevelopmental potentialmono-pronuclear zygotepronuclear characteristics
Type
Research Article
Information
Zygote , Volume 32 , Issue 6 , December 2024 , pp. 459 - 465
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Copyright
© The Author(s), 2024. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally to the research and preparation of the manuscript.

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