The basic mechanism of insulin secretion involves the coupling of glucose metabolism with secondary signals, which maintains insulin release for the duration of elevated blood glucose levels(Reference Oliveira, Latorraca and de Mello1).
In rodents, malnutrition during the critical stages of development causes a permanent loss of glucose sensitivity and secretory capacity in pancreatic islets(Reference Latorraca, Reis and Carneiro2), which is probably the result of alterations to the coupling of stimuli with insulin secretion. In pancreatic islets from rats fed a low-protein (LP) diet during intra-uterine life and/or lactation, reductions in glucokinase (Gck) and hexokinase (Hxk) activity and content(Reference Heywood, Mian and Milla3), decreases in Ca2+ uptake and/or Ca2+ efflux(Reference Latorraca, Carneiro and Mello4), and impairments in insulin secretion in response to activators of the cyclic AMP (cAMP)/protein kinase A (PKA) and phospholipase C (PLC)/protein kinase C (PKC) pathways(Reference Cherif, Reusens and Dahri5) have been observed.
During pregnancy, several reversible β-cell adaptations occur to compensate for the increased demand for insulin, and these adaptations include enhanced secretion and a lower threshold for glucose-stimulated insulin secretion(Reference Parsons, Brelje and Sorenson6–Reference Sorenson and Brelje8). Adaptations to insulin secretion and stimulation thresholds are mediated, at least in part, by changes in the activity/content of key enzymes involved in glucose metabolism (glucose transporter 2 (GLUT2), Gck and Hxk) as well as by modulation of the insulin secretory process (PLC/PKC and cAMP/PKA)(Reference Weinhaus, Stout and Sorenson9–Reference Weinhaus, Bhagroo and Brelje11).
Permanent damage to the secretory process, resulting from early protein restriction, may impair classical β-cell adaptation during pregnancy and contribute to the onset of diabetes. Thus, in the present study, we evaluated glucose homeostasis and investigated various steps involved in the coupling of stimuli with insulin secretion, particularly those that may be involved in impaired β-cell sensitivity to glucose and a reduced activation threshold for glucose-stimulated insulin secretion. The present study examined islets isolated from pregnant or non-pregnant rats that had been exposed to a low-protein (LPP and LPNP, respectively) or control (CP and CNP, respectively) diet from the fetal stage until adulthood, as well as islets isolated from pregnant and non-pregnant rats that had been protein-restricted during intra-uterine life and lactation but were then allowed to recover after weaning (RP and RNP, respectively).
Materials and methods
Animals and diets
The experimental procedures involving rats were performed in accordance with the guidelines of the Brazilian College for Animal Experimentation (COBEA) and were approved by the Ethics Committee at the Federal University of Mato Grosso (protocol no. 23 108·002104/08-5). Male and virgin female Wistar rats (85–90 d old) were obtained from the University's own breeding colony. Pairing was performed by housing males with females overnight (one male with four females), and pregnancy was confirmed by the examination of vaginal smears for the presence of sperm. Pregnant females were separated at random and maintained from the first day of pregnancy until the end of lactation on isoenergetic diets containing either 6 % protein (LP diet) or 17 % protein (control (C) diet). Spontaneous delivery took place at day 22 of pregnancy, and large litters were then reduced to eight pups when they were 3 d of age to ensure a standard litter size per mother. At weaning (fourth week after birth), females were divided into three groups: C, consisting of offspring born to and suckled by mothers fed a C diet and subsequently fed the same diet after weaning; LP, consisting of the offspring of mothers fed a LP diet and subsequently fed the same diet after weaning; R, consisting of the offspring of mothers fed a LP diet, but fed a C diet after weaning. At 90 d old, pairing was performed and the CNP and CP groups and the RNP and RP groups were fed a C diet, whereas the LPNP and LPP groups were maintained on a LP diet from the first day until the 19th day or from first day until the 15th day of pregnancy. The diets were isoenergetic, as described by Milanski et al. (Reference Milanski, Arantes and Ferreira12). During the experimental period, the rats had access to their respective diets and to water ad libitum and were housed at 22°C with a 12 h light–12 h dark cycle. In vivo studies have shown that pregnant rats become progressively resistant to insulin after day 16 of pregnancy(Reference Leturque, Ferré and Sabatin13). On the other hand, it has been observed that in isolated perfused pancreases, the glucose stimulation threshold reached a nadir of 3·25 mm-glucose on days 12 and 15 of pregnancy, and insulin secretion peaked at 4-fold on day 15 of pregnancy(Reference Sorenson and Brelje8). Thus, one group of rats was maintained on the dietary regimen until day 15 of pregnancy, when the islets were isolated to evaluate insulin secretion. The second group of rats was maintained on the study diet until 19 d of pregnancy, during which the glucose and insulin tolerance tests were performed to evaluate insulin sensitivity. After undergoing the insulin tolerance test, rats were anaesthetised with sodium thiopental and decapitated, and the body weights of fetuses were recorded. The remaining rats were weighed and euthanised, and blood was collected for biochemical analysis. Since it was not possible to evaluate all variables in the same animal, the number of individual experiments varied among the groups, but was representative of at least three different litters.
Glucose tolerance test
After a 12 h fast, glucose (200 g/l) was administered intraperitoneally at a dose of 2 g/kg body weight. Blood samples were obtained from the cut tip of the tail at 0, 30, 60 and 120 min post-injection for the determination of serum glucose and insulin concentrations. The trapezoidal method was used to calculate glucose and insulin responses during the glucose tolerance test. These responses were calculated by estimating the total area under the glucose (ΔG) and insulin (ΔI) curves(Reference Matthews, Altman and Campbell14).
Insulin tolerance test
After a 12 h fast, insulin (regular) was administered intraperitoneally at a dose of 1·5 U/kg body weight. Blood samples were obtained from the cut tip of the tail at 0, 5, 10 and 15 min post-injection for the determination of serum glucose concentrations. The glucose response during the insulin tolerance test was evaluated by the constant of disappearance of plasma glucose (Kitt) that was calculated from the slope of the fall in log-transformed plasma glucose between 0 and 15 min after insulin administration(Reference Lundbaek15), when the glucose concentration declined linearly.
Serum parameters
Blood samples were collected and allowed to clot. Sera were stored at − 20°C for the subsequent measurement of serum glucose by the oxidase–peroxidase method(Reference Trinder16), and serum albumin concentrations were determined by a colorimetric method(Reference Doumas, Watson and Biggs17). Serum insulin levels were determined by RIA(Reference Scott, Atwater and Rojas18).
Total insulin content and insulin secretion in isolated islets
Islets were isolated by collagenase digestion of the pancreas, as described by Boschero et al.(Reference Boschero, Szpak-Glasman and Carneiro19). For each experimental group, twenty islets were sonicated in 1 ml of distilled water and maintained at − 20°C until total insulin content determination by RIA(Reference Scott, Atwater and Rojas18).
Groups of five islets were first incubated for 45 min at 37°C in Krebs-bicarbonate buffer with the following composition: 115 mm-NaCl, 5 mm-KCl, 2·56 mm-CaCl2, 1 mm-MgCl2, 10 mm-NaHCO3, 15 mm-HEPES and 5·6 mm-glucose, supplemented with 3 g bovine serum albumin/l and equilibrated with a mixture of 95 % O2–5 % CO2 to give a pH of 7·4. This medium was replaced with fresh buffer, and then the islets were further incubated for 1·5 h with glucose concentrations of 2·8, 5·6, 8·3, 11·1 and 16·7 mm. The glucose concentration producing a response that was 50 % of the maximum (50 % effective concentration) was expressed as the mean negative logarithm (pD2).
In the second series of the experiments, insulin secretion was measured in response to the following: (1) glucose (2·8 or 8·3 mm) in the absence and presence of isobutylmethylxanthine (IBMX 1 mm; Sigma) and (2) glucose (2·8 or 8·3 mm) in the absence or presence of phorbol-12-myristate 13-acetate (PMA; 100 nm; Sigma). The insulin released was measured by RIA using rat insulin as the standard(Reference Scott, Atwater and Rojas18).
Glucose metabolism
The rate of glucose metabolism was measured in islets freshly isolated by collagenase digestion.
2-Deoxy-d-glucose uptake
Groups of twenty islets were incubated in 100 μl of buffered Krebs-bicarbonate solution supplemented with 3 μCi [14C]2-deoxy-d-glucose (Amersham Biosciences) with enough unlabelled glucose (Sigma-Aldrich) to achieve a final concentration of 2·8 or 8·3 mm. Tubes were incubated in a shaking water-bath at 37°C, and transport was stopped after 2 h with 0·5 ml Trizol reagent (Invitrogen Life Science). Scintillation fluid (3 ml) was added to the vials. Specific radioactivity was determined by liquid scintillation spectrometry. Uptake was expressed as pmol/islet per h.
d-Glucose utilisation and oxidation
Glucose utilisation and glucose oxidation were measured in batches of twenty islets in final incubation volumes of 100 μl of buffered Krebs-bicarbonate solution supplemented with trace amounts of both [5-3H]glucose and d-[U-14C]glucose (3 μCi/ml) (Amersham Biosciences) as well as non-radioactive glucose at a final concentration of either 2·8 or 8·3 mm. The wells were suspended in 20 ml scintillation vials that were gassed with 95 % O2 and 5 % CO2 and capped airtight with rubber membranes. The vials were then shaken continuously for 2 h at 37°C in a water-bath. After incubation, 0·1 ml of 0·2 m-HCl and 0·2 ml NaCl were injected through the rubber cap into the glass cup containing the incubation medium and into the counting vial, respectively. After 1 h at 4°C, 10 ml of the scintillation fluid were added to NaCl, and radioactivity was counted. To determine glucose utilisation, cups were transferred to a fresh set of scintillation vials, each containing 500 μl of distilled water. The vials containing the islets were then incubated for 18 h to allow the 3H-labelled water in the incubation solution to equilibrate with the water at the base of the vial before the cups and islets were removed, and 10 ml of the scintillation fluid were added to the distilled water. Specific radioactivity was determined by liquid scintillation spectrometry. Rates of glucose utilisation and glucose oxidation were determined and expressed as pmol/islet per h.
Measurement of intracellular cyclic AMP content
The cAMP content of islets was determined with the cAMP Enzyme immunoassay system (Amersham cAMP Biotrak Enzyme immunoassay System #RPN225; Amersham Life Sciences) according to the manufacturer's instructions. Groups of twenty islets were incubated in Krebs solution supplemented with 2·8 or 8·3 mm-glucose in the presence of 250 μm-IBMX. The content of cAMP was measured by enzyme immunoassay.
Western blotting
After isolation by collagenase digestion of the pancreas and subsequent separation by handpicking, a pool of at least 500 clean islets from each experimental group was homogenised by sonication (15 s) in an anti-protease cocktail (10 mm-imidazole at pH 8·0, 4 mm-EDTA, 1 mm-ethylene glycol tetra-acetic acid, 0·5 g pepstatin A/l, 2 g aprotinin/l, 2·5 mg leupeptin/l, 30 mg trypsin inhibitor/l, 200 μm-dl-dithiothreitol and 200 μm-phenylmethylsulfonyl fluoride). After sonication, an aliquot of the extract was collected, and the total protein content was determined by the dye-binding protein assay kit (Bio-Rad Laboratories). Samples containing 50 μg of protein from each experimental group were incubated for 5 min at 80°C with 4 × concentrated Laemmli sample buffer (1 mm-sodium phosphate at pH 7·8, 0·1 % bromophenol blue, 50 % glycerol, 10 % SDS and 2 % mercaptoethanol) (4:1, v/v) and then run on 10 % polyacrylamide gels at 120 V for 30 min. Electrotransfer of proteins to nitrocellulose membranes (Bio-Rad) was done for 1 h at 120 V (constant) in buffer containing methanol and SDS. After checking the efficiency of transfer by staining with Ponceau S, the membranes were blocked with 5 % skimmed milk in Tween-Tris-buffered saline (TTBS) (10 mm-Tris, 150 mm-NaCl and 0·5 % Tween 20) overnight at 4°C. GLUT2, Gck, HxkI, adenylyl cyclase 3 (AC3), PKAα, PKCα and PLCβ1 were detected in the membranes after a 2 h incubation at room temperature with primary antibodies (diluted 1:500, v/v, in TTBS containing 3 % dry skimmed milk; Santa Cruz Biotechnology). The membranes were then incubated with a secondary specific IgG antibody (diluted 1:5000, v/v, in TTBS containing 3 % dry skimmed milk) for 2 h at room temperature. After incubation with a horseradish peroxidase-conjugated secondary antibody, enhanced chemiluminescence (SuperSignal West Pico) was used for detection by autoradiography. Band intensities were quantified by optical densitometry (Scion Image).
Statistical analysis
The results are presented as means and standard deviations. For the islet data, n refers to the number of experiments performed. To evaluate the effect of PMA or IBMX, values obtained from differences between insulin secretion in the presence and absence of each potentiator were considered. Levene's test for homogeneity of variances was initially used to check the fit of the data to the assumptions for parametric ANOVA. To correct for variance heterogeneity or non-normality, data were log-transformed(Reference Sokal and Rohlf20). Except for the body weight of offspring, which was analysed by one-way ANOVA, and glucose-stimulated insulin secretion, evaluated by three-way ANOVA (nutritional status, physiological status and glucose concentration), all results were analysed by two-way ANOVA (nutritional status and physiological status). Where necessary, these analyses were complemented by the least significant difference test to determine the significance of individual differences. The level of significance was set at P <0·05. Data were analysed using the Statistica Software package (StatSoft).
Results
Glucose homeostasis in pregnant rats recovered from early malnutrition
At the end of the recovery period and before pregnancy, the body weight in the R groups was significantly lower than in the C groups, but it was higher than in the LP groups (F 2,29= 106·5, P< 0·0001). During pregnancy, body-weight gain was higher in the pregnant groups than in the non-pregnant groups, independent of the nutritional status (F 1,29= 202·4, P< 0·0001). However, in pregnant rats, body-weight gain was lower in the LPP group than in the CP group (P< 0·05). In the RP group, body-weight gain did not differ from that of the CP and LPP groups. Thus, pregnant rats had higher final body weights than non-pregnant rats (F 1,29= 49·2, P< 0·0001), but the RP rats exhibited higher final body weights than the LPP rats and lower body weights than the CP rats (P< 0·05). The serum albumin level was lower in the LP groups than in the R and C groups, independent of the physiological status (F 2,19= 10·4, P< 0·001; Table 1).
Table 1 Initial and final body weight, body-weight gain, serum albumin and total protein concentrations from non-pregnant or pregnant rats maintained on a control diet (CNP and CP, respectively), a low-protein diet (LPNP and LPP, respectively) or recovered after weaning (RNP and RP, respectively) (Number of rats, mean values and standard deviations)
a,b,c,dMean values within a row with unlike superscript letters were significantly different (P< 0·05; least significant difference test).
* Mean values were significantly different between nutritional status (P< 0·05; two-way ANOVA).
The basal serum glucose levels as well as the mean total areas under the ΔG curves in response to a glucose load were lower in the pregnant groups than in the non-pregnant groups (F 1,28= 5·2, P< 0·03 and F 1,28= 39·2, P< 0·0001, respectively), independent of the nutritional status. Pregnancy did not enhance the basal serum insulin concentration in the R group, which had a lower value than the CP and LPP groups (P< 0·05). In contrast, the mean total areas under the ΔI curves were significantly increased by pregnancy only in the LP diet-fed group. The ΔI curve from the RP group was lower than that from the CP group (P< 0·05) and was similar to that of the LPP group. The ΔG:ΔI ratios were significantly affected by nutritional status, physiological status and by the interaction of the two (F 2,28= 4·4, P< 0·05, F 1,28= 12·1, P< 0·001, F 2,28= 4·8, P< 0·05, respectively). Thus, the ΔG:ΔI ratio of the LPNP group was higher compared with that of the other groups (P< 0·01). Pregnancy only reduced the ΔG:ΔI ratio in the LPP group (P< 0·01). To measure insulin sensitivity, we performed an insulin tolerance test to calculate glucose disappearance in response to an acute insulin dose. Insulin sensitivity was higher in the LPNP group than in the other groups (F 2,18= 15·3, P< 0·001). Moreover, Kitt in the RNP group was smaller than that in the CNP group (P< 0·05). Pregnancy induced insulin resistance in the CP (P< 0·05) and LPP (P< 0·001) groups but not in the RP group, which had Kitt similar to that in the RNP group (Table 2).
Table 2 Fasting serum glucose and insulin concentrations, total areas under the glucose (ΔG) and insulin (ΔI) curves, ΔG:ΔI ratio obtained from the intraperitoneal glucose tolerance test and glucose disappearance ratio (Kitt) obtained from the intraperitoneal insulin tolerance test from non-pregnant or pregnant rats maintained on a control diet (CNP and CP, respectively), a low-protein diet (LPNP and LPP, respectively) or recovered after weaning (RNP and RP, respectively) (Number of rats, mean values and standard deviations)
a,b,c,dMean values within a row with unlike superscript letters were significantly different (P< 0·05; least significant difference test).
* Mean values were significantly different between physiological status (P< 0·05; two-way ANOVA).
Fetuses from mothers undergoing protein deficiency up to the 12th week of age had lower body weights than those from the other groups (LPP = 1·5 (sd 0·2) g, RP = 4·0 (sd 0·4) g, CP = 3·0 (sd 0·2) g). Recovered rats had 30 % heavier fetuses than those of the C group (F 2,30= 187·20, P= 0·000).
Total insulin content and insulin secretion in islets from pregnant rats recovered from early malnutrition
Insulin secretion in the presence of 2·8 mm-glucose was greater in islets from the RNP rats than in islets from the LPNP and CNP rats. In the presence of 8·3 and 16·7 mm-glucose, insulin secretion in islets from the RNP and CNP rats was similar and greater, respectively, compared with the LPNP rats. The dose–response curve for glucose from the LPNP group was shifted to the right compared with the curves from the RNP and CNP groups (P< 0·01 and P< 0·05, respectively), and the latter two groups had similar glucose dose–response curves. There was no difference in insulin secretion between the pregnant groups in the presence of 2·8 and 8·3 mm-glucose. In the RP group, maximal insulin release was obtained at a dose of 16·7 mm-glucose, whereas in the LPP and CP groups, maximal insulin secretion occurred at a dose of 11·1 mm-glucose. Under conditions of 11·1 and 16·7 mm-glucose, insulin secretion in islets from the RP rats was greater compared with that from the LPP and CP rats. In islets from pregnant rats, the dose–response curves were shifted to the left compared with the curves for non-pregnant rats (F 2,12= 22·89, P< 0·01), except in the RP group, which had a curve similar to that of the RNP group. The half-maximal release concentrations of glucose for islets from the CNP, CP, LPNP, LPP, RNP and RPP groups were 9·4 (sd 1·5), 7·7 (sd 0·15), 11·2 (sd 0·1), 7·1 (sd 0·5), 8·8 (sd 0·62) and 10·4 (sd 0·15) mm-glucose, respectively (Fig. 1(A)–(C)).
Fig. 1 Glucose stimulation of insulin secretion by islets from the (A) control non-pregnant (CNP) and control pregnant (CP), (B) low-protein non-pregnant (LPNP) and low-protein pregnant (LPP) or (C) recovered non-pregnant (RNP) and recovered pregnant (RP) rats. (D) The total insulin content and the increment in glucose-stimulated insulin secretion (2·8 or 8·3 mm-glucose) by (E and F) isobutylmethylxanthine and (G and H) phorbol-2-myristate 13-acetate of islets isolated from the non-pregnant or pregnant rats that had been subjected to the control diet (CNP and CP, respectively) or the low-protein diet (LPNP and LPP, respectively) or those that had recovered after weaning (RNP and RP, respectively). Values are means, with standard deviations represented by vertical bars (n 3–8 experiments). a–rMean values with unlike letters were significantly different (P< 0·05; least significant difference test).
The islets from the LPNP and RNP groups exhibited similar total insulin contents, and these values were significantly lower than those for islets from the CNP group (P< 0·01 and P< 0·05, respectively). Pregnancy increased the total insulin content only in the C and R groups, but the islets from the RP group had lower total insulin contents than those from the CP group (P< 0·001; Fig. 1(D)).
At non-stimulatory (Fig. 1(E)) and stimulatory (Fig. 1(F)) glucose concentrations, the increase in insulin secretion induced by IBMX in islets from the LPNP and CNP groups was similar, and this increment was greater than that observed for islets from the RNP group (P< 0·0001). At a dose of 2·8 mm-glucose, the magnitude of insulin secretion induced by IBMX did not differ among the RP, LPP and CP groups. The potentiator effect of IBMX at a dose of 2·8 mm-glucose was greater in islets from the RP group than those from the RNP group (P< 0·001), lower in islets from the LPP group than those from the LPNP group (P< 0·01) and equal in islets from the CP and CNP groups. Upon increasing the glucose concentration to 8·3 mm, the magnitude of potentiation of IBMX was greater in islets from all pregnant rat groups compared with those from the non-pregnant rat groups (F 1,22= 101·2, P< 0·001).
At a dose of 2·8 mm-glucose, the increase in insulin secretion by PMA was greater in the RNP group than in the LPNP group (P< 0·01), but the increase in the RNP group was significantly lower compared with that in the CNP group (P< 0·001). Pregnancy enhanced the potentiator effect of PMA in all groups (F 1,23= 157·4, P< 0·001), but this effect was lower in islets from the RP group than in islets from the LPP and CP groups (P< 0·001), which exhibited a similar magnitude of insulin secretion (Fig. 1(G)). At a dose of 8·3 mm-glucose, the potentiation of insulin secretion by PMA in the islets from the LPNP group was lower than that exhibited by the islets from the CNP group (P< 0·05). The potentiator effect of PMA in islets from the RNP group did not differ from that exhibited by islets from the LPNP and CNP groups. The magnitude of potentiation of PMA was greater in islets from all pregnant rat groups compared with that in islets from the non-pregnant rat groups (F 1,19= 92·2, P< 0·001). However, PMA evoked a similar insulin secretion in islets from the LPP and CP groups, and both increases were greater than those exhibited by islets from the RP group (P< 0·05 and P< 0·01, respectively; Fig. 1(H)).
Expressions of glucose transporter 2, glucokinase and hexokinase, and glucose metabolism in islets from pregnant rats recovered from early malnutrition
The GLUT2 content was similar to that in islets from the RNP and LPNP groups, and both groups exhibited a significantly higher GLUT2 content than the CNP (P< 0·001) and CP groups (P< 0·001). Pregnancy did not modify the GLUT2 content in islets from the LPP and CP groups in relation to the LPNP and CNP groups, respectively, but it decreased the GLUT2 expression in islets from the RP group compared with those from the RNP group (P< 0·001; Fig. 2(A)). The Gck and Hxk contents were affected by nutritional status (F 2,12= 9·9, P< 0·05 and F 2,16= 4·1, P< 0·05, respectively). Thus, islets from the LPP and LPNP groups had higher Gck expression than islets from the RP, RNP, CP and CNP groups (Fig. 2(B)). In contrast, the Hxk expression was lower in islets from the RP and RNP groups compared with those from the other groups (Fig. 2(C)).
Fig. 2 (A) Glucose transporter 2, (B) glucokinase and (C) hexokinase concentrations detected by Western blot in islets isolated from non-pregnant or pregnant rats maintained on a control diet (CNP and CP, respectively), a low-protein diet (LPNP and LPP, respectively) or recovered after weaning (RNP and RP, respectively). Values are means, with standard deviations represented by vertical bars (n 3–5 experiments). a,b,cMean values with unlike letters were significantly different (P< 0·05; least significant difference test). * Mean values were significantly different between nutritional status (P< 0·05; two-way ANOVA).
The glucose uptake rate observed in the presence of 2·8 mm-glucose was similar in islets from the RNP and CNP groups, and both glucose uptake rates were lower in relation to that exhibited by islets from the LPNP group (P< 0·001; Fig. 3(A)). In the presence of 8·3 mm-glucose, islets from the RNP group exhibited a lower glucose uptake rate than islets from the CNP group (P< 0·05), a rate which was also lower than that of islets from the LPNP group (P< 0·001) (Fig. 3(B)). Regardless of the glucose concentration used for the glucose uptake assay, pregnancy increased the glucose uptake rates in islets from the RP (P< 0·001) and CP groups (P< 0·001 and P< 0·05, respectively) and reduced the rates in islets from the LPP group (P< 0·001) (Fig. 3(A) and (B)).
Fig. 3 (A, B) Glucose uptake, (C, D) glucose utilisation and (E, F) glucose oxidation rates in the presence of 2·8 or 8·3 mm-glucose (respectively) in islets isolated from non-pregnant or pregnant rats maintained on a control diet (CNP and CP, respectively), a low-protein diet (LPNP and LPP, respectively) or recovered after weaning (RNP and RP, respectively). Values are means, with standard deviations represented by vertical bars (n 8 experiments). a,b,c,d,eMean values with unlike letters were significantly different (P< 0·05; least significant difference test). * Mean values were significantly different between physiological status (P< 0·05; two-way ANOVA).
In the presence of either 2·8 (Fig. 3(C)) or 8·3 mm-glucose, 3H2O production was similar in all non-pregnant groups (Fig. 3(D)). When 2·8 mm-glucose was used for the assay, pregnancy decreased the glucose utilisation rate in islets from the CP and LPP groups in relation to those from the CNP (P< 0·001) and LPNP (P< 0·05) groups, respectively, but enhanced it in islets from the RP group compared with those from the RNP group (P< 0·001) (Fig. 3(C)). After increasing the glucose concentration to 8·3 mm, the glucose utilisation ratio increased in islets from the CP and RP groups compared with those from the CNP (P< 0·001) and RNP groups (P< 0·05), respectively, but not in islets from the LPP group compared with those from the LPNP group (Fig. 3(D)).
To examine the next step in glucose metabolism, we evaluated the glucose oxidation rate in the presence of 2·8 (Fig. 3(E)) and 8·3 mm-glucose (Fig. 3(F)). Under basal conditions, 14CO2 production was decreased in islets from the LNP and RNP groups compared with those from the CNP group (P< 0·05). Pregnancy improved this parameter in all groups (F 1,29= 260, P< 0·001), but the magnitude of this increment was higher in islets from the LPP group than in those from the CP and RNP groups. Thus, islets from the LPP and CP groups exhibited equivalent oxidation rates, and these rates were higher than those in islets from the RP group (P< 0·05). However, the 14CO2 production from the latter group was not significantly different from that in the CP group (Fig. 3(E)). Under stimulatory concentrations, the glucose oxidation rate was only altered by physiological status (F 1,26= 1398·0, P< 0·01). The islets from pregnant rats had higher glucose oxidation rates compared with the islets from non-pregnant rats (Fig. 3(F)).
Intracellular cyclic AMP content in islets from pregnant rats recovered from early malnutrition
The measurement of intracellular cAMP content was evaluated in the presence of 2·8 or 8·3 mm-glucose with IBMX (0·5 mm) to inhibit the degradation of this second messenger. In the first condition, the cAMP content was lower in islets from the LPNP group than in those from the RNP and CNP groups (P< 0·001). Pregnancy increased the intracellular cAMP content in all groups (F 1,17= 366·5, P< 0·001). However, islets from the RP group had a higher cAMP content than those from the LPP group (P< 0·001), and islets from the LPP group had a significantly higher cAMP content than those from the CP group (P< 0·001) (Fig. 4(A)). In the presence of 8·3 mm-glucose, the intracellular cAMP contents in islets from the RNP and LPNP groups were similar and significantly lower than that in islets from the CNP group (P< 0·001). Pregnancy enhanced the cAMP content in all groups (F 1,17= 254·7, P< 0·001), and no significant difference was observed among the LPP, RP and CP groups (Fig. 4(B)).
Fig. 4 Intracellular cyclic AMP (cAMP) content in the presence of (A) 2·8 or (B) 8·3 mm-glucose in islets isolated from non-pregnant or pregnant rats subjected to a control diet (CNP and CP, respectively), a low-protein diet (LPNP and LPP, respectively) or recovered after weaning (RNP and RP, respectively). Values are means, with standard deviations represented by vertical bars (n 10 experiments). a,b,c,d,eMean values with unlike letters were significantly different (P< 0·05; least significant difference test).
Expressions of adenylyl cyclase 3, protein kinase A-α, phospholipase C-β1 and protein kinase C-α in islets from pregnant rats recovered from early malnutrition
The protein content of AC3 in islets from the RNP and LPNP groups was higher than that in islets from the CNP group (P< 0·001). Pregnancy increased the AC3 expression by approximately 23 and 124 % in islets from the LPP and CP groups in relation to those from the LPNP (P< 0·01) and CNP groups (P< 0·001), respectively, and reduced the AC3 expression by approximately 21 % in islets from the RP group compared with those from the RNP group (P< 0·01) (Fig. 5(A)).
Fig. 5 (A) Adenylyl cyclase 3 (AC3), (B) protein kinase A-α (PKAα), (C) phospholipase C-β1 (PLCβ1) and (D) protein kinase C-α (PKCα) concentrations detected by Western blot in islets isolated from non-pregnant or pregnant rats maintained on a control diet (CNP and CP, respectively), a low-protein diet (LPNP and LPP, respectively) or recovered after weaning (RNP and RP, respectively). Values are means, with standard deviations represented by vertical bars (n 3–4 experiments). a,b,c,d,eMean values with unlike letters were significantly different (P< 0·05; least significant difference test). * Mean values were significantly different between nutritional status (P< 0·05; two-way ANOVA).
The content of PKAα was modified by nutritional status (F 2,12= 16·60; P< 0·001). Thus, the islets from the R (RNP and RP) and LP (LPNP and LPP) rats had PKAα contents that were 156 and 118 % higher than the islets from the C rats (CNP and CP), respectively (Fig. 5(B)).
The PLCβ1 content was higher in islets from the RNP and LPNP groups than that in islets from the CNP group (P< 0·01 and P< 0·05, respectively). The islets from the LPP and CP groups had higher PLCβ1 protein expression than those from the LPNP (P< 0·05) and CNP groups (P< 0·001), respectively, whereas the islets from the RP group exhibited similar PLCβ1 protein expression levels to those from the RNP group. The PLCβ1 protein expression did not differ among the three pregnant groups (Fig. 5(C)). The protein expression of PKCα in islets from the RP rats was lower than that in islets from the RNP group (P< 0·01), whereas its content was similar in islets from the CP, LPP, CNP and LPNP groups (Fig. 5(D)).
Discussion
In the present study, recovered non-pregnant rats demonstrated the indicators of in vivo (fasting serum insulin concentration, ΔI and ΔG:ΔI ratio) and in vitro (dose–response curve) insulin secretions similar to control non-pregnant rats, despite a deficit in the total insulin content per islet in relation to the control non-pregnant rats. Furthermore, recovered non-pregnant rats were more insulin resistant than low-protein and control non-pregnant rats, but their glucose tolerance (accessed by ΔG and fasting glucose concentration) was similar to the glucose tolerance of those two groups. In a previous study(Reference Weinhaus, Stout and Sorenson9), male low-protein and recovered rats exhibited increased insulin sensitivity, normal total insulin content per islet and impairment of insulin secretion. Differences between sexes may be related to a diverse hormonal milieu that could be modulating insulin secretion and sensitivity. The present results are contrary to the observation that females fed a low-protein diet during early life become relatively more insulin deficient and glucose intolerant(Reference Chamson-Reig, Thyssen and Hill21). However, alterations in the metabolic pathway that contribute to glucose intolerance may be related to the age of the animals studied. For example, Chamson-Reig et al. (Reference Chamson-Reig, Thyssen and Hill21) observed animals at 130 d of age, whereas the present study was conducted with animals that were 90 d old.
Islets from female rats fed a low-protein diet up to the 12th week of age displayed greater glucose uptake but similar glucose utilisation compared with islets from the control rats. Thus, we hypothesised that glucose that is not metabolised by glycolysis is shifted to other pathways, such as the pentose-phosphate shunt, providing β-cells with either NADP as a high-energy substrate for insulin secretion or ribose substrates for the synthesis of nucleotides. Interestingly, the expression of Gck and Hxk did not correlate with the glucose utilisation rate and was not consistent with insulin secretory profiles observed in islets from low-protein and recovered non-pregnant rats. These results suggest that Gck and Hxk activity is more important than Gck and Hxk expression for determining the glycolytic flux and the shift of the dose–response curve. This supposition is reinforced by a report that islets from rats recovered from early malnutrition after weaning displayed reduced Gck and Hxk activity(Reference Heywood, Mian and Milla3). Finally, in contrast to evidence suggesting that GLUT2 may be an essential enabling factor for glucose responsiveness(Reference Johnson, Newgard, LeRoith, Taylor and Olefsky22), in the present study, islets from the low-protein and recovered non-pregnant rat groups that overexpressed GLUT2 exhibited different glucose responsiveness.
The ability of cAMP to potentiate insulin secretion stimulated by glucose through the activation of PKA is well documented(Reference Yang and Gillis23). In the present study, islets from the low-protein and recovered non-pregnant rats exhibited reduced cAMP content despite increased AC3 content. Because cAMP generation dependent on the AC3 isoform is modulated by Ca2+/calmodulin complexes(Reference Tian and Laychock24), and Ca2+ handling is compromised in islets from low-protein non-pregnant rats(Reference Latorraca, Carneiro and Mello4), it is reasonable to speculate that AC activity was reduced, at least in islets from low-protein non-pregnant rats. The lower potentialisation magnitude elicited by IBMX on insulin secretion in the face of increased PKA expression observed in islets from the low-protein and recovered non-pregnant rats also indicates impaired PKA activity, but to a greater degree in islets from the recovered non-pregnant rats than from the low-protein non-pregnant rats.
The PLC/PKC pathway was also altered in islets from the recovered and low-protein non-pregnant rats. The PLC content was increased in both groups, possibly in an attempt to increase inositol 1,4,5-triphosphate and diacylglycerol levels. The PKC content was not modified, but its activity was reduced, especially in islets from the low-protein non-pregnant rats, considering the weak PMA potentialisation on insulin secretion.
Curiously, the pattern of insulin resistance(Reference Leturque, Ferré and Sabatin13, Reference Freemark25) and the functional changes of the endocrine pancreas(Reference Sorenson and Brelje8) typical of pregnancy were observed in the low-protein pregnant rats but not in the recovered pregnant rats. Even so, recovered pregnant rats did not develop gestational diabetes or glucose intolerance, possibly because insulin sensitivity and insulin secretion were reciprocally related. However, fetuses from the recovered pregnant rats exhibited greater body weights than those from the low-protein and control pregnant rats, which is an indication of disrupted maternal metabolism.
To address the increased insulin demand, islets should combine mechanisms that enhance their secretory capacity and reduce the threshold for glucose-stimulated insulin secretion. Total insulin content per islet, a measure that reflects insulin biosynthesis and/or β-cell mass, must be amplified during pregnancy(Reference Sorenson and Brelje8). In the present study, islets from the recovered pregnant rats increased the total insulin content, but it was not enough to reach the value of islets from the control pregnant rat group. However, islets from the recovered pregnant rats exhibited greater glucose responsiveness and lower glucose sensitivity than islets from the control rats. In contrast, islets from the low-protein pregnant rats had lower total insulin content and reduced glucose responsiveness but similar glucose sensitivity in relation to islets from the control pregnant rats. These results suggest alterations in one or more mechanisms of insulin secretion.
The glucose-stimulated insulin response curves shifted to lower glucose concentrations during pregnancy, has been associated with an increase in glucose utilisation due to the enhanced activity and/or content of GLUT2, Gck and Hxk, as well as to an increase in glucose oxidation(Reference Weinhaus, Stout and Sorenson9). Contrary to these assumptions, the Gck and Hxk contents in the present study were not altered in any pregnant groups, but the dose–response curve for islets from the recovered pregnant rats was shifted to the right in relation to islets from the low-protein and control pregnant rats. The GLUT2 content and glucose uptakes were similar across the islets from the recovered and control pregnant rats. Thus, there are no correlations between GLUT2, Gck and Hxk expression and glucose sensitivity in islets from the pregnant rats. In addition, islets from the control pregnant rats did not show enhancement of GLUT2, Gck or Hxk contents, but exhibited glucose uptake, utilisation and oxidation compatible with increased glucose sensitivity. It is interesting to emphasise that Weinhaus et al. (Reference Weinhaus, Stout and Sorenson9) observed similar Gck and Hxk expression levels in control islets and islets at day 15 of pregnancy on a protein equivalence basis. Increased Gck and Hxk expressions were observed only when enzyme levels were normalised on the basis of DNA. Considering that our glucose utilisation and oxidation results in islets from the recovered and low-protein rats did not correlate with the changes observed in glucose sensitivity, we investigated other pathways of stimulus–secretion coupling that could explain the secretory pattern exhibited by islets from the low-protein and recovered rats.
The expected rise in cAMP generation previously reported in pregnancy(Reference Weinhaus, Bhagroo and Brelje11) was observed in the present study, even in islets from the recovered pregnant rats with decreased AC3 contents. The cAMP generation profile of islets from the recovered and low-protein pregnant rats indicated increased AC3 activity. As previously reported(Reference Milanski, Arantes and Ferreira12), pregnancy did not increase the PKA content. However, taking into account the increased cAMP generation and the potentiator effect of IBMX in low glucose concentrations, it is possible to infer that PKA activity was reduced in islets from the pregnant rats subjected to prolonged protein restriction, as well as in islets from the pregnant rats rescued from early protein restriction. Hence, the pattern of glucose sensitivity observed in islets from the recovered and low-protein pregnant rats did not correlate with alterations in the cAMP/PKA pathway.
The PLC/PKC system is involved in the reduction of the stimulatory threshold glucose concentration and in the potentiation of insulin secretion during pregnancy(Reference Sorenson and Brelje8), and this system is impaired in a model of protein restriction after weaning(Reference Ferreira, Barbosa and Stoppiglia26). The PLC content was similar across all islets from the pregnant rats, and PKC expression was reduced in islets from the recovered pregnant rats. In addition, PMA was less efficient in potentiating insulin secretion in the recovered pregnant rats, indicating a lower PKC activity in that group. Thus, the PLC/PKC system in islets from the recovered pregnant rats appears to be severely attenuated.
Conclusion
These results suggest that protein restriction in early life impairs the classical β-cell adaptive changes during pregnancy and disrupts maternal metabolism, but not enough to promote the onset of gestational diabetes. The loss of the adaptive capacity of islets from the recovered pregnant rats apparently resulted from the uncoupling of glucose metabolism and the amplifying signals of the secretory process, as well as from a severe attenuation of the PLC/PKC pathway. Alterations exhibited by islets from the pregnant rats undergoing sustained protein deficiency up to the 12th week of age appeared to result more from reduced islet mass and/or insulin biosynthesis than from impairment of the stimulus–secretion coupling process.
Acknowledgements
The authors are grateful to Celso Roberto Afonso for excellent technical assistance and to Dr Maria Alice Rostom de Mello for kindly providing the [14C]2-deoxy-d-glucose for glucose metabolism assays in isolated islets. This study was supported by the Brazilian foundations CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, grant no.: 305155/2004-0), FAPEMAT (Fundação de Amparo à Pesquisa do Estado de Mato Grosso, grant no.: 0786/2006) and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, grant no.: PROCAD 022/2007). This work is part of a dissertation presented by Letícia Martins Ignácio de Souza as a partial requirement for the Master's degree in Biosciences at the Faculty of Nutrition, Universidade Federal de Mato Grosso. L. M. I.-S. and S. R. R. carried out the assays in isolated islets, B. L. B. conducted the in vivo experiments. V. C. A., R. V. V. and F. F. performed the RIA and analysis and interpretation of the data. A. C. B., E. M. C. and M. A. d. B. R. contributed equally to this work in various aspects of this study. M. Q. L. conceived and designed the study. L. M. I.-S. drafted the manuscript along with the other authors. All authors read and approved the final manuscript. The authors declare that they have no conflict of interest.
