Introduction
East Asia harbours a diverse range of relic monotypic genera dating back to the Paleozoic, Neogene and even Cretaceous periods (Tang et al., Reference Tang, Matsui, Ohashi, Dong, Momohara, Sonia, Qian, Yang, Ohsawa, Lu, Grote, Krestov, Ben, Werger, Robertson, Hobohm, Wang, Peng, Chen, Wang, Su, Zhou, Lin, He, Yan, Zhu, Hu, Yang, Li, Tomita, Wu, Yan, Zhang, He, Yi, Gong, Song, Song, Li, Zhang, Han, Shen, Huang, Luo and Jordi2018). Presently, some of these genera have degenerated into unique single-species populations that are reduced in size and limited to specific habitats in tropical and subtropical China. Notable examples include Ginkgo biloba L., Metasequoia glyptostroboides Hu & W. C. Cheng, Thuja sutchuenensis Franch, Taiwania cryptomerioides Hayata, Cathaya argyrophylla Chun et Kuang, and Nypa fruticans Wurmb (Tang and Ohsawa, Reference Tang and Ohsawa2002; Tang et al., Reference Tang, Yang, Ohsawa, Momohara, Hara, Chen and Fan2011, Reference Tang, Yang, Ohsawa, Arata, Yi, Kevin, Song, Zhang and He2015; He et al., Reference He, Tang, Wu, Wang, Ohsawa and Yan2015; Qian et al., Reference Qian, Yang, Tang, Momohara, Yi and Ohsawa2016; Lovly and Merlee Teresa, Reference Lovly and Merlee Teresa2016). Over the last 50 years, over 200 species have become extinct, and nearly 5000 plant species are currently threatened (Volis, Reference Volis2016). The decline of these wild plant populations in China during the 20th and 21st centuries is primarily attributed to habitat loss, environmental pollution and global climate change (Xu et al., Reference Xu, Rena, Wei, Ouyang, Li, Guo, Wen, Long, Wang and Hui2021) and their conservation is essential for biodiversity maintenance (Brown et al., Reference Brown, Owen, Peters, Zhang and Soffker2015; Qin et al., Reference Qin, Zhao, Yu, Liu, Liu, Xia, Peng, Li, Zhang, He, Yin, Lin, Liu, Hou, Liu, Liu, Cao, Li, Chen, Jin, Gao, Chen, Ma, Geng, Jin, Chang, Jiang, Cai, Zang, Wu, Ye, Lai, Liu, Lin and Xue2017). In addition to threats from their limited geographic range, small population size and fragile habitats, continuing the decline of such species is exacerbated primarily by low fruit set, seed set, seed germination and seedling survival rates (Neaves et al., Reference Neaves, Eales, Whitlock, Hollingsworth, Burke and Pullin2015). Understanding their life history and how they respond to their environment is, therefore, crucial for their conservation.
Seed germination is a critical phase in the plant life cycle, susceptible to various factors and often a bottleneck for natural regeneration (Jimenez-Alfaro et al., Reference Jimenez-Alfaro, Silveira, Fidelis, Poschlod and Commander2016; Jiang et al., Reference Jiang, Xie, Chai, Tang, Jiang, Qin and Wei2022). Research has shown that seeds of mangrove plants respond differently to their environment in various habitats (Su et al., Reference Su, Shen, Cai, Jiang, Yang and Xu2021). Key indicators of seed germination include initial germination time, germination rate and emergence rate, which are crucial for seed-to-seedling transition and natural regeneration (Rosbakh and Poschlod, Reference Rosbakh and Poschlod2015). These differences in seed germination traits reflect the adaptation strategies of different species (Li et al., Reference Li, Zhou, Lu, Li, Sun and Meng2020).
Mangroves grow in the intertidal region between land and sea and are affected by a variety of environmental factors such as light, moisture, salinity, tides, temperature, soil and wind (Zhang, Reference Zhang2019). Current studies on the relationship between mangroves and the environment have focused on shade tolerance, salt tolerance and flooding tolerance of seedlings (Lv et al., Reference Lv, Li, Yang, Zhang and Deng2019). Therefore, through simulation experiments, the seed germination characteristics of endangered mangrove plants are studied to explore the limiting factors of seedling regeneration, which can provide a basis for elucidating the causes of their endangerment and formulating conservation strategies (Li et al., Reference Li, Zhou, Lu, Li, Sun and Meng2020; Jiang et al., Reference Jiang, Xie, Chai, Tang, Jiang, Qin and Wei2022).
Vivipary in plants refers to a phenomenon in which sexually reproduced offspring germinate while still attached to the mother plant (Robert et al., Reference Robert, Oste, van der Stocken, de Ryck, Quisthoudt, Kairo, Dahdouh-Guebas, Koedam and Schmitz2015). This is mostly manifested in mangrove plants, which occur in tropical and subtropical intertidal zones and encounter harsh environmental conditions such as high salinity, high temperatures, waterlogging, hypoxia and tidal waves (Zhou et al., Reference Zhou, Cai, Fu, Hong, Shen and Li2016). Extensive research has shown that high salt inhibits growth, while low salt promotes it (Liu et al., Reference Liu, Qin and Zheng2017). Both viviparous and non-viviparous propagules prefer low salt environments, but some require specific salinity for germination (Robert et al., Reference Robert, Oste, van der Stocken, de Ryck, Quisthoudt, Kairo, Dahdouh-Guebas, Koedam and Schmitz2015). Viviparous propagules can survive prolonged flooding and benefit from tidal actions (Liu et al., Reference Liu, Yang, Liu, Chen and Jiang2022). Excessive flooding affects colonization and survival due to waterlogged soil, which reduces photosynthesis and growth (Yan et al., Reference Yan, Wang and Huang2004; Zhou et al., Reference Zhou, Weng, Su, Ye, Qu and Li2023). Light plays a crucial role in propagule colonization. Viviparous propagules with chlorophyll in their epidermis can photosynthesize, while those without cannot (Liu and Liao, Reference Liu and Liao2013). Propagules respond to varying light intensity by adjusting leaf area and chlorophyll content (Ulqodry et al., Reference Ulqodry, Matsumoto, Okimoto, Nose and Zheng2014). Interspecies differences exist in the effect of shading on embryonic axis survival (Duke and Watkinson, Reference Duke and Watkinson2002). The water column's salinity and pH change continuously, and different mangrove species cope with flooding with varying levels of success (Liu et al., Reference Liu, Yang, Liu, Chen and Jiang2022).
N. fruticans, a true mangrove plant of the genus Nypa in the Arecaceae family, thrives in calm estuaries and coastal areas. It is primarily found on the landward side of mangrove forests in low salinity areas (Zakaria et al., Reference Zakaria, Aslezaeim and Sofawi2017). N. fruticans is an ecologically and economically vital component of East Asian mangrove ecosystems (Lovly and Merlee Teresa, Reference Lovly and Merlee Teresa2016). Climate change and human activities have drastically reduced its global distribution (Jian et al., Reference Jian, Ban, Ren and Yan2010). Currently, its natural range is restricted to the paleotropics (India-Australia), and in China, it is naturally distributed only on Hainan Island (Zhang et al., Reference Zhang, Zhong, Lv, Fang and Cheng2022). Field surveys revealed that there are only four natural populations of N. fruticans in China, which are naturally distributed in Haikou, Wenchang, Qionghai and Wanning on Hainan Island. Only the Wanning population showed a cluster distribution in patches, while other populations were scattered. N. fruticans is a protected species and is listed in the Red Book of Chinese Plants, with its protection status upgraded from national level 3 to level 2 in 2021 (Fu, Reference Fu1991; Zhang et al., Reference Zhang, Zhong, Lv, Fang and Cheng2023).
Previous research on the reproductive characteristics of N. fruticans has revealed that it has a low fruit set rate of 37.58% and low germination rates of 1.85% (Rozainah and Aslezaeim, Reference Rozainah and Aslezaeim2010). Through the preliminary investigation, there were very few seedlings under the N. fruticans forest in China, indicating that there were serious seedling regeneration restrictions. However, the potential causes for this phenomenon are not clear. At present, there are few studies on the adaptability of N. fruticans seeds, so it is necessary to obtain their responses to environmental factors such as light, salinity and flooding time through single-factor gradient experiments, which will provide basic data for the in-depth study of their environmental adaptation mechanisms.
Therefore, we hypothesized that seed germination and seedling regeneration of N. fruticans are related to environmental factors and tested this hypothesis by (i) comparing fruit and seed traits of different N. fruticans populations, (ii) comparing seed germination characteristics of different N. fruticans populations and germination characteristics of seeds from a single population in multiple in situ N. fruticans habitats and (iii) exploring the effects of different environmental factors (light intensity, salinity and flooding time) on N. fruticans seed germination.
Materials and methods
Materials
N. fruticans is a tufted evergreen shrub that grows a height of 3–9 m. The rhizome is creeping and grows horizontally in the seashore mud. The infructescence is capitate, containing 32–38 mature carpels. Each small fruit develops from one carpel. Mature carpels are drupe-like, brown, shiny, obovate, 9–11 cm long, slightly compressed and hexagonal. Each small fruit contains one seed, which is round and measures about 3–4 cm long and 4 cm wide. N. fruticans has fruit all year round, but most fruit is produced in June and July.
Through preliminary field surveys, we found that there were four natural populations of N. fruticans plants on Hainan Island, with the population number being about 9319 trees (Table 1). Of these, seedlings were found in the understory of all populations, except the Qionghai population. Therefore, three natural populations of N. fruticans (Haikou, Wenchang and Wanning) were selected for the study.
Table 1 Geographic distribution and resource status of N. fruticans
In order to obtain sufficient seeds, 50 healthy plants were randomly selected as sampling mother trees and tagged at each of the three N. fruticans populations before the start of the experiment. To prevent the seeds from falling and being washed away by seawater, netting was placed over the infructescence and secured before ripening. When the infructescence was ripe and split, the netting was then transported back to the laboratory along with the infructescence.
Methods
Fruiting traits of N. fruticans from different populations
Before flowering, 100 trees of N. fruticans were randomly labelled from each population. After infructescence ripening, the natural fruit set rate was calculated for each population and repeated three times. Fruit set refers to the percentage of the total number of flowers in the tree that actually set fruit in the natural state. Twenty-five infructescences were randomly selected from each population and the infructescence diameter was measured with a tape measure. After infructescence dehiscence, the seed set rate of each infructescence was calculated. The seed set rate refers to the number of full seeds as a percentage of the total number of seeds. The maximum longitudinal length and the maximum transverse length of each fruitlet were measured with vernier callipers (Li et al., Reference Li, Zhou, Lu, Li, Sun and Meng2020; Jiang et al., Reference Jiang, Xie, Chai, Tang, Jiang, Qin and Wei2022).
Germination characteristics of N. fruticans seeds under natural environmental conditions
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(1) Germination characteristics of seeds from different populations under the common garden experiment
A common garden was constructed in Lingao County, Hainan Province, where there is a mangrove nursery of the Forestry Academy of Hainan Province. From April 2021 to June 2021, 20 infructescences were randomly selected from the Haikou, Wenchang and Wanning populations, respectively. The infructescences were ripe and cracked, and all seeds were packed into seedling bags (Glorious Yi Brand, Shandong Jianyang Biotechnology Co.) with nutrient-rich soil (Stanley Brand, Stanley Agricultural Group Inc.). The seedling bags were divided into three groups according to different populations and placed under the common garden experiment. The experiment was replicated three times. Seed germination rates and emergence rates of seedlings from different populations were counted after 1 and 3 months, respectively.
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(2) Germination characteristics of seeds from a single population in three in situ N. fruticans habitats
From April 2021 to June 2021, 30 infructescences were randomly selected from the Wanning population. Each infructescence was covered with 1 net pocket, totalling 30 net pockets (Summer Snow Brand, Qingdao Summer Snow Fishing Tackle Co.). All net pockets were equally divided into three groups and fixed on mother trees in the natural habitat of N. fruticans in Haikou, Wenchang and Wanning, respectively. The experiment was replicated three times. Seed germination rates and emergence rates of seedlings from different populations were counted after 1 and 3 months, respectively.
Effect of different environmental factors on the seed germination of N. fruticans
Six hundred and ninety full and pest-free N. fruticans seeds were randomly selected from the Wanning population and inserted into culture bags filled with sandy soil. Four light intensity treatment groups, 8 salinity treatment groups and 11 flooding treatment groups were designed, and single-factor experiments for light, salinity and flooding were established separately (Table 2). Different light intensity treatment levels were adjusted using shade nets (Lvandi, Greenland Shade Co.) with different shading effects. Salinity manipulations were carried out using sea salt (from Dongfang City Saltwork) and tap water. Salinity levels were checked and adjusted weekly. To reflect semi-diurnal tides, watering was divided into two injection/discharge cycles per day (Table 3). The flooding and draining for each group were controlled with mini water pumps (Type: HQB – 2000, rated power: 24 W, pump head:1.8 m and pump flow: 1400 L/h). Each group had 30 seeds (10 seeds per treatment in 3 replications). The experiment was conducted from March 2022 to June 2022, and the test site was located in the mangrove nursery base of the Hainan Academy of Forestry (Hainan Academy of Mangrove).
Table 2 Experimental design for the germination of N. fruticans seeds
Table 3 Time of injection and discharge of water per day
Data analysis
Six indices were selected to measure fruiting traits: infructescence diameter, number of small fruits per infructescence, maximum longitudinal length, maximum transverse length, fruit setting rate and seed-setting rate. Eight indices were selected to measure seed germination characteristics: initial germination time, germination duration, germination rate, initial emergence time, emergence duration, emergence rate, seedling height and number of leaves. The criterion for germination was the protrusion of the terminal bud from the pericarp, and the absence of germination of the embryonic axis for five consecutive days marked the end of the germination experiment. Meanwhile, the criterion for seedling emergence was the emergence of the first pair of leaves from the terminal bud, and the absence of seedling emergence for five consecutive days marked the end of the seedling emergence experiment.
Initial germination time was the length of time from the beginning of the germination test to the first germination event. Initial emergence time was defined as the length of time from the beginning of the germination test to the first seeding emergence. Germination duration was the length of time from the first germination event to the end of the germination test, and emergence duration was the length of time from the first seedling emergence to the end of the seedling emergence test. The germination (GP) and emergence rates (SR) were calculated as follows:
In the above equation, Ga represents the number of seeds that have germinated, Gn represents the number of seeds supplied for testing, and Ns represents the final number of seedlings that survived.
The mean and standard error (SE) of three replicates were calculated. Data on all measurement indicators were analysed for the differences among different treatments by the analysis of variance. If the difference was significant at P < 0.05, a Duncan test was employed to determine the potential source of the difference. All statistical analyses were performed with SPSS, version 16.0 (SPSS Inc., Chicago, IL, USA). Statistical significance was defined as P < 0.05.
Results
Fruiting traits of N. fruticans from different populations
The diameter and number of fruitlets per N. fruticans infructescence were 26.49 ± 2.13 cm and 82.16 ± 4.22, respectively. The infructescence diameter and number of small fruits from Wanning were significantly greater than those from Haikou and Wenchang. The maximum longitudinal length and the maximum transverse length of small fruits were 9.59 ± 0.11 cm and 7.19 ± 0.14 cm, respectively, and the differences in the maximum longitudinal length and the maximum transverse length among the various populations were not significant. In addition, the natural fruiting and seed-setting rates were only 21.11 ± 8.75 and 40.40 ± 5.31%, respectively, with the fruiting and seed-setting rates from Wanning also being significantly higher than those from Haikou and Wenchang (Table 4).
Table 4 Fruiting traits of N. fruticans from different populations
Different letters indicate a significant difference (P < 0.05).
Germination characteristics of N. fruticans seeds under the natural environment
Under the same common garden conditions, the differences in germination and seedling emergence rates among the three populations were highly significant. The germination and emergence rates of the Wanning population were significantly greater than those of the Haikou and Wenchang populations, and the order of germination rates and emergence rates among the various populations was Wanning > Wenchang > Haikou. Mean values of germination and seedling emergence rates were 36.58 ± 5.16 and 22.99 ± 3.74%, respectively (Fig. 1A). Under different common garden experiments, the germination rate of seeds from the same population did not differ significantly among three in situ habitats, though interestingly the emergence rate differed significantly. The seedling emergence rate in the Wanning habitat was significantly higher than that in the remaining two habitats (Fig. 1B).
Figure 1. Germination characteristics of N. fruticans seeds in the natural environment. Different letters indicate a significant difference (P < 0.05).
Effect of light intensity on N. fruticans seed germination
Under different light intensity treatments, the initial germination time, germination duration and germination rate of N. fruticans seeds were not significantly different (p > 0.05), though the initial emergence time, emergence duration, emergence rate, seedling height and number of leaves did differ among groups (p < 0.05).
All treatment groups started germination on the first day of the experiment and lasted for about 3 days. With increasing light intensity, the initial emergence time and emergence duration first decreased then increased, with minimum values of 6.33 and 8 days at 60% and maximum values of 9.67 and 13.33 days at 100% of light intensity, respectively (Fig. 2A). The germination rate and seedling emergence rate showed a trend of increasing and then decreasing, where the germination rate remained above 70% among all light treatments, and the seedling emergence rate had a maximum value of 70% at 60% light intensity, and the emergence rate (50%) under full light conditions was significantly smaller than the remaining three treatments (Fig. 2B). Seedling height showed a trend of increasing and then decreasing, with a maximum value of 34.53 cm at 60% light intensity and a minimum value of 21.75 cm at 100% (Fig. 2C). The number of leaves tended to decrease gradually, with a maximum value of 4.25 leaves at 20% light intensity and a minimum value of 1.75 leaves at 100% (Fig. 2D).
Figure 2. Effect of light intensity on N. fruticans seed germination. Different letters indicate a significant difference (P < 0.05).
Effect of salinity on N. fruticans seed germination
All germination indicators of N. fruticans seeds were significantly different under different salinity treatments (P < 0.05). Each indicator gradually increased in the salinity range of 0–20%, and the initial germination time, germination duration, initial emergence time and emergence duration increased by 12, 19, 24 and 26 days in a 20% saline solution compared with the control (Fig. 3A).
Figure 3. Effect of salinity on N. fruticans seed germination. Different letters indicate a significant difference (P < 0.05).
In the salinity range of 0–25‰, both germination and emergence rates first increased, then decreased among treatments, with maximum values of 63.33 and 50% at 5‰, respectively. In the salinity range of 5–25‰, these two indicators among treatments were significantly lower than those in the salinity range of 0–5‰. The germination rate and seedling emergence rate were 0 at salinities above 25‰ (Fig. 3B). Both seedling height and leaf number showed a trend of increasing and then decreasing, with maximum values at salinities of 5 and 10‰, respectively. Seedling height and leaf number under salinities of 0–10‰ were significantly greater than those under 10–20‰ (Figs. 3C and 3D).
Effect of flooding time on N. fruticans seed germination
Under different flooding treatments, the germination indicators of N. fruticans seeds were significantly different, with the exception of initial germination time (P < 0.05). All treatment groups started germination on the first day. With the increase of flooding time, the germination duration, initial emergence time and emergence duration first decreased, then increased. They all yielded minimum values under the conditions of 8 h/day, then they rose significantly up to maximum values under 22 h/day. The germination duration, initial emergence time and emergence duration were not significantly different among treatments under the conditions of 2–10 h/day flooding time and were significantly lower than those under the conditions of 12–22 h/day (Fig. 4A).
Figure 4. Effect of flooding time on N. fruticans seed germination. Different letters indicate a significant difference (P < 0.05).
The germination and emergence rates gradually increased among treatments at flooding times of 2–8 h/day, where the germination rate of all treatments had maximum values at 8 h/day, while the emergence rate had a maximum at both 6 and 8 h/day. The germination rate and seedling emergence rate gradually decreased among treatments at 8–22 h/day and were significantly lower than those at 2–8 h/day (Fig. 4B). Both seedling height and leaf number showed an increasing trend followed by a decreasing trend, with both reaching a maximum at 8 h/day. Seedling height was significantly greater under 6–12 h/day than the rest of the treatment groups, and leaf number was significantly greater under 2–8 h/day than under 10–22 h/day (Figs. 4C and 4D).
Discussion
Fruiting traits of N. fruticans in China under natural conditions
Fruiting rates, seed-setting rates, germination rates and seedling survival rates are crucial factors in determining the life history and population renewal of plants. Fruit and seed-setting rates are closely linked to plant mating patterns and pollination efficiency (Yang et al., Reference Yang, Wang and Ma2020). In our study, we observed that the fruiting and seed-setting rates of N. fruticans were relatively low, a phenomenon shared with other endangered plants like Rhododendron changii (Fang) Fang and Sonneratia × hainanensis W. C. Ko & al. These low rates might be attributed to N. fruticans' limited reproductive capabilities (Pluntz et al., Reference Pluntz, Coz, Peyrard, Pradel, Choquet and Cheptou2018; Zhang et al., Reference Zhang, Yang, Long, Li and Lv2019). Research indicates that N. fruticans predominantly relies on outcrossing for sexual reproduction; however, it also exhibits some selfing tendencies (Mantiquilla et al., Reference Mantiquilla, Abad, Barro, Basilio and Silvosa2015). Moreover, the flowering period exhibits dichogamy, where pistils mature before stamens. This temporal mismatch, combined with factors like short pollination windows and weather-related disruptions, hampers the successful pollination and seed development of N. fruticans, significantly affecting its reproduction (Valdes et al., Reference Valdes, Lima and Noblick2021).
For plant populations, a potential result of habitat fragmentation is the disruption of pollination systems, causing a decline in reproductive success as small populations become less attractive to pollinators (Winter et al., Reference Winter, Lehmann and Diekmann2008). Similar studies such as single-plant seed production of Sanicula europaea L. decreased when populations were reduced (Kolb and Lindhorst, Reference Kolb and Lindhorst2006). Fruit set was significantly lower in small populations of Catasetum viridiflavum Hook than in large populations (Donaldson et al., Reference Donaldson, Nanni, Zachariades and Kemper2010). In the present study, the Wanning population exhibited significantly higher rates of fruiting and seed-setting rates compared to the other two populations. This may be related to its heterogamous pollination and population size. Pollinating insects play an important role in the reproductive process of N. fruticans populations (Valdes et al., Reference Valdes, Lima and Noblick2021). Smaller populations will be less attractive to pollinators and their reproductive outputs will be lower compared to larger populations (Su et al., Reference Su, Shen, Cai, Jiang, Yang and Xu2021; Jiang et al., Reference Jiang, Xie, Chai, Tang, Jiang, Qin and Wei2022). Our field surveys confirmed this, with the Wanning population being the largest, with over 2000 plants, while Haikou and Wenchang populations each contained fewer than 200 plants. This underscores the impact of population size on N. fruticans' population renewal. The large-scale decline in N. fruticans populations can be attributed to extreme climatic conditions and human activities (Zhang et al., Reference Zhang, Zhong, Lv, Fang and Cheng2022). Studies reveal that N. fruticans, once distributed across a wide range, now survive only in the paleotropics due to the extreme climates triggered by Quaternary glaciations (Chen, Reference Chen2016). During this period, N. fruticans populations substantially declined, leading to a loss of genetic diversity. Additionally, N. fruticans is a widely utilized mangrove species, subjected to overharvesting and long-term exploitation. The construction of reclamation projects, mariculture bases and real estate developments can result in the extensive destruction of N. fruticans populations (Jian et al., Reference Jian, Ban, Ren and Yan2010).
Seed germination under natural conditions is influenced by factors such as geography, climatic factors and genetic differences (Sudrajat, Reference Sudrajat2016). Influenced by geography, the same tree species in different regions produce rich genetic variation due to geography, and this is reflected in seed germination characteristics through stable genetic material (Hao et al., Reference Hao, Zhou, Han, Zhai and Chen2021). Previous studies have shown that seed germination and germination speed of Pinus tabuliformis Carrière vary greatly in different regions, which may be caused by genetic factors or due to environmental influence (Wang et al., Reference Wang, Yan, Zhang, Ding, Zhang and Yang2015). The high variability in seed germination traits exhibited by Cercidiphyllum japonicum Siebold & Zucc was a result of a combination of their own attributes, habitat characteristics and geographic isolation (Li et al., Reference Li, Zhou, Lu, Li, Sun and Meng2020). In this study, the germination and seedling emergence rates of the Wanning population were significantly higher than those of the Haikou and Wenchang populations, which indicated that there were significant population differences in seed germination traits of N. fruticans. The reason may be that the long-term growth and reproduction of different N. fruticans populations in localized environments may contribute to the genetic differentiation of these populations, which ultimately leads to differences in seed germination and seedling growth characteristics among different populations. We also found that the germination rate of seeds from the same population did not differ significantly among three in situ habitats, though interestingly the emergence rate differed significantly. The seedling emergence rate in the Wanning habitat was significantly higher than that in the remaining two habitats. This suggests that the differences in germination characteristics among N. fruticans populations may be due to genetic variation among populations and are also influenced by seed source geography and habitat factors.
Effect of light intensity on N. fruticans seed germination
Plant species exhibit varying requirements for light during seed germination, with effects ranging from promotion to inhibition or no significant impact. Some seeds are highly light-sensitive and necessitate light for germination (Liu et al., Reference Liu, Ma, Li, Zhuang, Du, Xing and Liu2016; Zhang et al., Reference Zhang, Yang, Long, Li and Lv2019). Under darkness and constant temperature, the newly collected seeds of S. × hainanensis and S. ovata Backer did not completely germinate, and increased light promoted seed germination and radicle growth. Exposing the seeds to light for 12 h/day is optimal for their seed germination (Zhang et al., Reference Zhang, Yang, Long, Li and Lv2019; Ren et al., Reference Ren, Zhang, Zhang and Wang2021). Likewise, there were significant differences in the germination rates of Bruguiera gymnorhiza and Bruguiera sexangula (Lour.) Poir. seeds under different conditions of shading intensity (Zhang et al., Reference Zhang, Yang, Long, Li and Lv2019). In our study, we experimented with different light gradients by employing artificial shading to observe N. fruticans seed germination. Surprisingly, we found no significant difference in the initial germination time, germination duration and germination rate of N. fruticans seeds under different light intensities. This behaviour can be attributed to the nature of N. fruticans seeds. Unlike most plant seeds, N. fruticans seeds complete their germination while still attached to the parent tree (Su et al., Reference Su, Shen, Cai, Jiang, Yang and Xu2021; Jiang et al., Reference Jiang, Xie, Chai, Tang, Jiang, Qin and Wei2022; Zhang et al., Reference Zhang, Zhong, Lv, Fang and Cheng2022). The phenomenon of vivipary is the most common in the plant kingdom with mangrove plants, which can be divided into vivipary and cryptovivipary (Robert et al., Reference Robert, Oste, van der Stocken, de Ryck, Quisthoudt, Kairo, Dahdouh-Guebas, Koedam and Schmitz2015). In the former, the embryonic axis extends beyond the pericarp and gradually grows into a columnar seedling. Hence, this type of propagule is often called an embryonic axis (Zhou et al., Reference Zhou, Cai, Fu, Hong, Shen and Li2016). Mangrove plants such as B. gymnorhiza and B. sexangula belong to this type. In the latter, the embryonic axis only breaks through the seed coat but remains enclosed within the fruit. Cryptoparasitic plants include mangrove plants such as N. fruticans and Aegiceras corniculatum (L.) Blanco (Yan et al., Reference Yan, Wang and Huang2004). In this case, light has less effect on seed germination in N. fruticans.
Under natural conditions, not all seeds have the same fate after leaving the parent tree (Liu et al., Reference Liu, Huang, Guo, Wang D, Wang, Wang, Ma and Liu2019). The transition from seeds to seedlings is further subjected to rigorous environmental sieves, leading to only a rare seedling surviving to achieve natural renewal (Han et al., Reference Han, Zhang, Nurmati and Yang2021). Our study revealed that seedling emergence rates and seedling height in N. fruticans tended to increase up to a certain point and then decrease as light intensity varied. The highest values were observed at 60% light intensity, while full light conditions yielded the lowest values. This implies that moderate shading is optimal for N. fruticans seedling growth, with both high and low light conditions being unfavourable. Observations in the field also confirm that N. fruticans seedlings mainly thrive in the forest understory, making light a key factor affecting seedling regeneration.
Effect of salinity on N. fruticans seed germination
The seed germination stage is particularly sensitive to salt stress (Zhang et al., Reference Zhang, Yang, Long, Li and Lv2019). Mangrove plants, specialized in growing in saline environments, require a certain level of salt throughout their life cycle. While they can endure freshwater or low salinity conditions, high salt levels hinder their growth (Lv et al., Reference Lv, Li, Yang, Zhang and Deng2019). Our results indicate that increasing salinity significantly prolonged the initial germination time, germination duration, initial emergence time and emergence duration of N. fruticans seeds. At low salinity (5‰), the germination rate, seedling emergence rate, plant height and leaf number were not significantly different from the control (0‰), indicating that low salt concentrations had little effect on N. fruticans seed germination. However, as salinity increased, these indicators decreased significantly. When salt concentrations exceeded 25‰, the N. fruticans seed germination rate dropped to zero. This phenomenon could be attributed to osmotic effects and ion toxicity caused by high salinity. Osmotic effects inhibit water uptake by seeds, while ion toxicity inhibits cell growth and division (Orlovsky et al., Reference Orlovsky, Japakova, Zhang and Volis2016; Saberali and Moradi, Reference Saberali and Moradi2019). This low salt-promoted, high salt-inhibited behaviour is also observed during seed germination in other mangrove non-viviparous plants like S. × hainanensis and S. ovata, which display an optimal salinity of 2.5‰ (Zhang et al., Reference Zhang, Yang, Long, Li and Lv2019; Ren et al., Reference Ren, Zhang, Zhang and Wang2021). In contrast, certain viviparous plants, such as B. gymnorhiza and Rhizophora stylosa, exhibit seed germination suitable for moderate (10‰) and high (20‰) salinity conditions, respectively (Mo et al., Reference Mo, Fan and He2001). N. fruticans falls between non-viviparous and viviparous plants, with an optimum salinity of 5‰. Notably, in the natural habitat, the seawater salinity of N. fruticans exceeds 15‰, demonstrating that the salinity in its native environment far exceeds the requirements for N. fruticans seed germination. Therefore, salinity simulation experiments confirmed that salinity acts as a limiting factor for both seed germination and seedling regeneration in N. fruticans.
Effect of flooding time on N. fruticans seed germination
Tides are a crucial environmental factor affecting mangrove plant growth, primarily influencing seed dispersal and distribution. Different types of seeds respond differently to tidal influences (Zhang, Reference Zhang2018). As a true mangrove plant, N. fruticans is also affected by tidal levels (Zhang et al., Reference Zhang, Zhong, Lv, Fang and Cheng2022). Generally, mangrove seedlings are submerged in a fully submerged state during most of the tidal cycle. Prolonged inundation time and greater inundation depth during high tides are key factors contributing to low seedling survival rates (Liu et al., Reference Liu, Yang, Liu, Chen and Jiang2022).
Numerous studies have shown that the appropriate duration of flooding facilitates the germination of mangrove plant seeds, but prolonged flooding results in slow or even no seed germination (Chen et al., Reference Chen, Lin and Wang2006; Liu et al., Reference Liu, Qin and Zheng2017, Reference Liu, Yang, Liu, Chen and Jiang2022). In our study, we observed no significant difference in the initial germination time, germination duration, initial emergence time and emergence duration of N. fruticans seeds when flooding time was less than 10 h/day. However, when inundation exceeded 10 h/day, these time indicators significantly lengthened, and germination and emergence rates declined substantially. The most favourable conditions were observed at 8 h/day of inundation. This suggests that 8 h/day is the optimal flooding time for N. fruticans seed germination, and 10 h/day acts as a critical threshold. Beyond this threshold, seed germination and seedling establishment are significantly inhibited. The main reason for this phenomenon is that propagules undergo anaerobic respiration under prolonged water immersion, producing and accumulating alcohol, which leads to cellular toxicity (Zhang et al., Reference Zhang, Liao and Guan2011; Liu et al., Reference Liu, Yang, Liu, Chen and Jiang2022). Our field monitoring found that the daily tidal cycle in the natural habitat of N. fruticans exceeded 8 h/day. In addition, other scholars' field investigations found that out of 162 N. fruticans seedlings, only three seedlings eventually grew into young trees, while most of the seedlings were washed away at high tide. This suggests that the flooding time dynamic plays a crucial role in the life cycle of N. fruticans.
Conservation strategies for natural populations of the endangered plant N. fruticans
To protect the genetic diversity of the endangered mangrove plant N. fruticans and foster natural renewal and population expansion, we propose the following conservation strategies in response to the factors contributing to its endangerment.
First, we suggest increasing investment in scientific research and technology to address seed source problems and expand population size. This involves several key initiatives:
1. Employing assisted pollination techniques, bagging tests and pollen cryopreservation to mitigate pollen limitations and low seed germination rates caused by overlapping flowering periods.
2. Utilizing traditional selection and molecular breeding methods, incorporating both asexual and sexual approaches, to develop efficient breeding techniques for N. fruticans, thereby increasing the number of high-quality populations.
3. Introducing N. fruticans germplasm resources from populations with high genetic diversity, while considering the potential for genetic contamination, to enhance the genetic diversity and germplasm sources. This should be complemented by establishing a N. fruticans germplasm nursery to produce high-quality seedlings at the scale.
Secondly, enhancing habitat restoration and in situ protection is vital to create a suitable ecological environment. This involves taking the following actions:
1. Implementing measures such as tree species transformation, pest control, tidal ditch restoration and light beach restoration to rehabilitate degraded N. fruticans habitats, enabling natural population renewal.
2. Designating key protection areas within N. fruticans habitats to prevent disturbances from human activities through regular patrolling. Utilizing nets over fruit-bearing N. fruticans and fencing under the N. fruticans forest to reduce the destructive effects of tides on seeds, further enhancing the plant's natural renewal capabilities.
3. In cases where N. fruticans populations are small within in situ conservation areas, transplanting numerous seedlings from established resource beds to replace degraded populations can be employed while adjusting stand density for improved restoration and protection effects.
Finally, we recommend optimizing relocation protection and field return strategies, followed by continuous monitoring and post-care. These measures should be carried out as follows:
1. Identify suitable relocation sites by conducting detailed assessments of ecological factors, such as climate, tide patterns, soil quality, vegetation and benthic organisms within N. fruticans' current habitat, combined with the necessary conditions for seed germination and seedling growth.
2. Domesticating seedlings in the field, after they meet release criteria from the nursery, involves continuous environmental monitoring and adjustments to reduce the impact of endangerment factors and enhance seedling survival rates.
3. Introduce a significant number of N. fruticans seedlings into natural or semi-natural habitats suitable for their distribution through artificial propagation. This will help establish new populations with sufficient genetic resources to adapt to evolving conditions and sustain natural renewal.
Conclusion
In summary, our analysis of N. fruticans' seed traits and germination characteristics under natural conditions has led us to conclude that there is a significant limitation in seedling regeneration. This limitation was confirmed through controlled environmental experiments, where we identified light, salinity and flooding as the primary factors constraining seedling regeneration. These findings have significant implications for the preservation and restoration of N. fruticans populations. Based on these observations, we recommend a comprehensive approach to conserve and recover N. fruticans populations, with a focus on scientific research and technology, habitat restoration, in situ protection, relocation and continuous monitoring. These strategies are vital for the self-renewal of this endangered mangrove plant and the expansion of its population size.
Acknowledgements
This work was financially supported by the Hainan Provincial Scientific Research Institute technology innovation special project (jscx202017). We would like to thank Dr Daniel Petticord at the University of Cornell for his assistance with English language and grammatical editing of the manuscript.
Author contributions
M.W.Z. and C.R.Z. planned the experiments and conceived the paper. All authors contributed to the writing and editing of the manuscript.
Competing interests
The authors declare that there is no conflict of interest.
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