Reduced exposure to sweet taste has been proposed to reduce sweet food preferences and intakes, but the evidence to support these associations is limited. This randomised controlled trial investigated the effects of a whole-diet sweet taste intervention for 6 days, on subsequent pleasantness, desire for, and sweet food intakes. Participants (n 104) were randomised to increase (n 40), decrease (n 43), or make no change to (n 21) their consumption of sweet-tasting foods and beverages for six consecutive days. Pleasantness, desire to eat, sweet taste intensity and sweet food intakes were assessed on days 0 and 7. One-hundred-and-two (98%) participants completed the study, and self-reported adherence with the dietary interventions was moderate-good (M=66-72/100mm), with instructions to decrease sweet food consumption reported as more difficult than the other diets (smallest (t(81)=2.45, p=.02, Mdiff=14/100mm, SE=2mm). In intention-to-treat analyses, participants in the decrease sweet food consumption group reported higher sweet taste intensity perceptions at day 7 compared to day 0 (F(2,101)=4.10, p=.02, Mdiff=6/100mm, SE=2mm). No effects were found for pleasantness (F(2,101)=2.04, p=.14), desire to eat (F(2,101)=1.49, p=.23) or any of the measures of sweet food intake (largest F(2,101)=2.53, p=.09). These results were confirmed in regression analyses which took self-reported adherence to the diets into account. Our findings suggest that exposure to sweet taste does not affect pleasantness, desire for, or intakes of, sweet-tasting foods and beverages. Public health recommendations to limit the consumption of sweet-tasting foods and beverages to reduce sweet food preferences may require revision.