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Genotypic insights to Panton–Valentine leukocidin positive methicillin-resistant Staphylococcus aureus isolated from cattle mastitis

Published online by Cambridge University Press:  06 May 2024

Muhammad Ijaz ,
Nauman Zaheer Ghumman ,
Muhammad Umar Javed ,
Arslan Ahmed ,
Hamza Rasheed ,
Farwa Anwaar ,
Muhammad Jawad Sabir  and
Iqra Muzammil
Muhammad Ijaz*
Affiliation:
Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore, Pakistan
Nauman Zaheer Ghumman
Affiliation:
Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore, Pakistan
Muhammad Umar Javed
Affiliation:
Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore, Pakistan
Arslan Ahmed
Affiliation:
Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore, Pakistan
Hamza Rasheed
Affiliation:
Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore, Pakistan
Farwa Anwaar
Affiliation:
Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore, Pakistan
Muhammad Jawad Sabir
Affiliation:
Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore, Pakistan
Iqra Muzammil
Affiliation:
Department of Veterinary Medicine, University of Veterinary and Animal Sciences, Lahore, Pakistan
*
Corresponding author: Muhammad Ijaz; Email: [email protected]
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Abstract

In this research communication we investigate the prevalence and antimicrobial susceptibility of S. aureus harboring virulent genes responsible for mastitis in cattle of Punjab, Pakistan. A total of 690 milk samples were collected from commercial dairy farms for analysis of the prevalence of subclinical and clinical mastitis and isolation of S. aureus. Virulence ability and methicillin resistance in S. aureus (MRSA) was determined by targeting the pvl (the gene for Panton–Valentine leukocidin) and mecA genes, respectively. A total of 175 S. aureus isolates exhibiting prevalence of pvl gene (6.28%) and mecA gene (22.28%) were determined. Antimicrobial susceptibility testing of pvl positive and negative MRSA against different classes of antibiotics revealed 100% resistance against β-lactams while 100% sensitivity towards tylosin and linezolid.

Keywords

Antimicrobial resistancedairy cattleleukocidinmastitispvl
Type
Research Article
Information
Journal of Dairy Research , Volume 91 , Issue 2 , May 2024 , pp. 211 - 213
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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