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Tracing of Helicobacter pylori in the middle ear and mastoid mucosa of patients under 18 years of age with chronic otitis media (with and without cholesteatomas)

Published online by Cambridge University Press:  02 October 2023

Francisco Javier González-Eslait Open the ORCID record for Francisco Javier González-Eslait [Opens in a new window] ,
Pedro Abel Blanco-Sarmiento ,
Kabethliz Bejarano-Domínguez ,
José María Barreto  and
Emelina Ruiz-Tejada
Francisco Javier González-Eslait*
Affiliation:
Department of General Surgery, Section of Otorhinolaryngology, Universidad del Valle, Cali, Colombia Department of General Surgery, Section of Otorhinolaryngology, Clínica Imbanaco, Cali, Colombia Department of General Surgery, Section of Otorhinolaryngology, Hospital Universitario del Valle “Evaristo García,” Cali, Colombia
Pedro Abel Blanco-Sarmiento
Affiliation:
Department of General Surgery, Section of Otorhinolaryngology, Clínica Imbanaco, Cali, Colombia
Kabethliz Bejarano-Domínguez
Affiliation:
Department of External Consultation, Fundación Clínica Infantil Club Noel, Cali, Colombia
José María Barreto
Affiliation:
Department of General Surgery, Section of Otorhinolaryngology, Universidad del Valle, Cali, Colombia Department of General Surgery, Section of Otorhinolaryngology, Clínica de Otorrinolaringología y Cirugía Plástica, Cali, Colombia
Emelina Ruiz-Tejada
Affiliation:
Department of General Surgery, Section of Otorhinolaryngology, Universidad del Valle, Cali, Colombia Department of General Surgery, Section of Otorhinolaryngology, Clínica de Otorrinolaringología y Cirugía Plástica, Cali, Colombia Department of General Surgery, Section of Otorhinolaryngology, Fundación Valle del Lili, Cali, Colombia
*
Corresponding author: Francisco Javier González-Eslait; Email: [email protected]
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Abstract

Objective

It has been estimated that about 5 million people of those affected with otitis media have cholesteatoma, however, its pathophysiology is unclear. In this study we aimed to detect Helicobacter pylori via polymerase chain reaction and real-time polymerase chain reaction in young patients with chronic otitis media.

Methods

Patients included in our prospective cross-sectional study had middle-ear/mastoid inflammation and underwent surgical procedures. Middle-ear mucosa samples were collected, and genomic DNA was extracted for H pylori detection by polymerase chain reaction and real-time polymerase chain reaction analyses. Sociodemographic data and gastroesophageal reflux symptoms were analysed.

Results

We included 49 patients with mean age of 12.7 ± 3.8 years. Twenty per cent of the patients were diagnosed with cholesteatoma. No increase in H pylori-amplified fluorescence was observed, indicating absence of H pylori.

Conclusion

Due to the absence of amplification for H pylori and the fact that albumin was amplified in all samples, we conclude that H pylori does not appear to be a causal factor.

Keywords

CholesteatomaotitisHelicobacter pylori
Type
Main Article
Information
The Journal of Laryngology & Otology , Volume 138 , Issue 5 , May 2024 , pp. 503 - 506
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of J.L.O. (1984) LIMITED

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Footnotes

Francisco Javier González-Eslait takes responsibility for the integrity of the content of the paper

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