Introduction
Parental depression correlates with offspring emotional and behavioural problems (Lovejoy et al. Reference Lovejoy, Graczyk, O'Hare and Neuman2000; Kane & Garber, Reference Kane and Garber2004; Barker et al. Reference Barker, Copeland, Maughan, Jaffee and Uher2012). This has been reported in studies using categorical measures to identify depressed parents (Fendrich et al. Reference Fendrich, Warner and Weissman1990; Lieb et al. Reference Lieb, Isensee, Hofler, Pfister and Wittchen2002), and continuous measures of depressive symptoms (Davies & Windle, Reference Davies and Windle1997; Cummings et al. Reference Cummings, Keller and Davies2005). Such findings indicate the presence of one or more of three mechanisms: First, exposure to parental depressive symptoms may play a role in the development of offspring psychopathology. This could occur via negative parenting practices associated with depression (Kendler, Reference Kendler1996; Burt et al. Reference Burt, Van Dulmen, Carlivati, Egeland, Alan Sroufe, Forman, Appleyard and Carlson2005; Elgar et al. Reference Elgar, Mills, McGrath, Waschbusch and Brownridge2007), or via learning processes such as imitation or modelling (Bandura, Reference Bandura1977). Second, exposure to offspring psychopathology may increase parental depressive symptoms. For example, irritable or oppositional children may be difficult to parent and form positive relationships with, and this may affect the emotional wellbeing of parents. Third, parental depressive symptoms and offspring psychopathology may share a common aetiology.
Researchers have reported that parental depression/depressive symptoms predict offspring psychopathology (Fendrich et al. Reference Fendrich, Warner and Weissman1990; Davies & Windle, Reference Davies and Windle1997; Lieb et al. Reference Lieb, Isensee, Hofler, Pfister and Wittchen2002; Cummings et al. Reference Cummings, Keller and Davies2005), and offspring psychopathology predicts parental depression (Tamplin et al. Reference Tamplin, Goodyer and Herbert1998; Tan & Rey, Reference Tan and Rey2005; Wilkinson et al. Reference Wilkinson, Harris, Kelvin, Dubicka and Goodyer2013). Longitudinal research shows these relationships can be reciprocal, with maternal depressive symptoms prospectively predicting the development of child problem behaviours, and vice versa (Gross et al. Reference Gross, Shaw and Moilanen2008a , Reference Gross, Shaw, Moilanen, Dishion and Wilson b ; Gross et al. Reference Gross, Shaw, Burwell and Nagin2009).
A major problem in interpreting research linking parent and child psychopathology is that in most cases parents and children are genetically related, so associations may be confounded by shared genes. Depression is under genetic influence in adulthood (Sullivan et al. Reference Sullivan, Neale and Kendler2000) and childhood (Rice et al. Reference Rice, Harold and Thapar2002; Thapar & Rice, Reference Thapar and Rice2006), so genetic factors could explain associations between parent and offspring emotional problems. Furthermore, genetic effects overlap across traits (Krueger et al. Reference Krueger, Hicks, Patrick, Carlson, Iacono and McGue2002; Kendler et al. Reference Kendler, Prescott, Myers and Neale2003; McAdams et al. Reference McAdams, Rowe, Rijsdijk, Maughan and Eley2012; Bolhuis et al. Reference Bolhuis, McAdams, Monzani, Gregory, Mataix-Cols, Stringaris and Eley2014), so genetic commonalities could explain associations between parental depression and any heritable offspring outcome. Ultimately, intergenerational genetically informative data are required to properly assess the nature of the parent–child associations.
Children-of-twins (CoT) studies
Studying twins and their offspring allows researchers to identify whether associations between parent and child phenotypes are genetic and/or environmental in nature (Fischer, Reference Fischer1971; Gottesman & Bertelsen, Reference Gottesman and Bertelsen1989; D'Onofrio et al. Reference D'Onofrio, Turkheimer, Eaves, Corey, Berg, Solaas and Emery2003; Silberg & Eaves, Reference Silberg and Eaves2004; McAdams et al. Reference McAdams, Neiderhiser, Rijsdijk, Narusyte, Lichtenstein and Eley2014). The utility of a CoT sample has been described in detail elsewhere (McAdams et al. Reference McAdams, Neiderhiser, Rijsdijk, Narusyte, Lichtenstein and Eley2014), but is briefly described here. Because monozygotic (MZ) twins are genetically identical, their offspring are as genetically related to their parents’ co-twin as they are to their own parent. The relationship between a child and their parent's sibling is known as the avuncular relationship. In MZ twin families, if the parent–child correlation is greater than the avuncular correlation, then this indicates the presence of an association between parent and offspring phenotypes above and beyond familial confounding of genes and the extended family environment (e.g. the association is potentially attributable to parenting). If there is no difference then transmission is thought to be familial. The comparison between avuncular correlations in MZ v. dizygotic (DZ) families gives insight into the nature of familial effects. If the MZ avuncular correlation is larger, then genetic factors are implied.
Two CoT studies have investigated the relationship between parental depression and offspring depression and conduct problems (Silberg et al. Reference Silberg, Maes and Eaves2010; Singh et al. Reference Singh, D'Onofrio, Slutske, Turkheimer, Emery, Harden, Heath, Madden, Statham and Martin2011). Results from both indicated that the association between parent and child depression remained after accounting for familial confounds. One of the studies found that the association between parental depressive symptoms and adolescent conduct problems persisted after accounting for familial confounds (Silberg et al. Reference Silberg, Maes and Eaves2010), whereas the other did not (Singh et al. Reference Singh, D'Onofrio, Slutske, Turkheimer, Emery, Harden, Heath, Madden, Statham and Martin2011). Interestingly, neither study found evidence for genetic transmission running from parental depression to offspring depression, but both found evidence for genetic transmission from parental depression to conduct problems.
Adoption studies
Studying children adopted at birth provides another geneticall -informed method of examining intergenerational associations – when adoptive parents are genetically unrelated to their child, parent–child correlations are not confounded by shared genotype. Conversely, biological parents of children adopted at birth provide only their genes (and intrauterine environment), so any correlation between their phenotype and that of their child's cannot be attributed to a (post-adoption) environmental effect (assuming any selective placement is controlled for). Researchers have reported that adoptive-parent depression correlates with offspring depression (Cadoret et al. Reference Cadoret, Ogorman, Heywood and Troughton1985; Marmorstein et al. Reference Marmorstein, Iacono and McGue2012) but not substance use (Marmorstein et al. Reference Marmorstein, Iacono and McGue2012) in young adopted adults, and with offspring depression and disruptive behaviour in adopted adolescents (Tully et al. Reference Tully, Iacono and McGue2008). Using adoption data from the Early Growth and Development Study (EGDS), researchers have also shown that adoptive-parent depressive symptoms predicts child fussiness at age 18 months (Natsuaki et al. Reference Natsuaki, Ge, Leve, Neiderhiser, Shaw, Conger, Scaramella, Reid and Reiss2010), externalizing problems in toddlers aged 27 months (Pemberton et al. Reference Pemberton, Neiderhiser, Leve, Natsuaki, Shaw, Reiss and Ge2010), and internalizing and externalizing problems in early childhood (Kerr et al. Reference Kerr, Leve, Harold, Natsuaki, Neiderhiser, Shaw and Reiss2013; Laurent et al. Reference Laurent, Leve, Neiderhiser, Natsuaki, Shaw, Fisher, Marceau, Harold and Reiss2013a , Reference Laurent, Leve, Neiderhiser, Natsuaki, Shaw, Harold and Reiss b ).
Using data from EGDS (Leve et al. Reference Leve, Neiderhiser, Shaw, Ganiban, Natsuaki and Reiss2013), researchers have shown that birth parent depressive symptoms have no main effect on the development of fussiness in toddlers (age 9–18 months; Natsuaki et al. Reference Natsuaki, Ge, Leve, Neiderhiser, Shaw, Conger, Scaramella, Reid and Reiss2010), a borderline significant effect on externalizing problems in toddlerhood (age 27 months; Pemberton et al. Reference Pemberton, Neiderhiser, Leve, Natsuaki, Shaw, Reiss and Ge2010), and predict externalizing but not internalizing problems in early childhood (ages 18–54 months; Kerr et al. Reference Kerr, Leve, Harold, Natsuaki, Neiderhiser, Shaw and Reiss2013).
The current study
Some CoT and adoption findings are beginning to converge in a coherent story. For example, associations between parental depressive symptoms and offspring emotional problems remain after controlling for familial confounds (shared genes and extended family environment) (Cadoret et al. Reference Cadoret, Ogorman, Heywood and Troughton1985; Tully et al. Reference Tully, Iacono and McGue2008; Silberg et al. Reference Silberg, Maes and Eaves2010; Singh et al. Reference Singh, D'Onofrio, Slutske, Turkheimer, Emery, Harden, Heath, Madden, Statham and Martin2011; Marmorstein et al. Reference Marmorstein, Iacono and McGue2012; Kerr et al. Reference Kerr, Leve, Harold, Natsuaki, Neiderhiser, Shaw and Reiss2013; Laurent et al. Reference Laurent, Leve, Neiderhiser, Natsuaki, Shaw, Fisher, Marceau, Harold and Reiss2013a ). However, other results are inconsistent, with some studies finding an association between parental depression and offspring externalizing behaviours above and beyond familial confounds (Tully et al. Reference Tully, Iacono and McGue2008; Pemberton et al. Reference Pemberton, Neiderhiser, Leve, Natsuaki, Shaw, Reiss and Ge2010; Silberg et al. Reference Silberg, Maes and Eaves2010; Kerr et al. Reference Kerr, Leve, Harold, Natsuaki, Neiderhiser, Shaw and Reiss2013) where others do not (Singh et al. Reference Singh, D'Onofrio, Slutske, Turkheimer, Emery, Harden, Heath, Madden, Statham and Martin2011; Marmorstein et al. Reference Marmorstein, Iacono and McGue2012). Some findings stand out as unusual and in need of further examination. For example, parent depressive symptoms appear to have a non-significant genetic relationship with offspring internalizing problems but a significant association with offspring externalizing problems (Silberg et al. Reference Silberg, Maes and Eaves2010; Singh et al. Reference Singh, D'Onofrio, Slutske, Turkheimer, Emery, Harden, Heath, Madden, Statham and Martin2011; Kerr et al. Reference Kerr, Leve, Harold, Natsuaki, Neiderhiser, Shaw and Reiss2013). Furthermore, no genetically informative study has yet tested for bidirectional effects between parental depressive symptoms and offspring psychopathology.
In the present study, we follow the advice of others (Rutter et al. Reference Rutter, Pickles, Murray and Eaves2001) in using complementary but distinct research designs to examine our research questions. We use a CoT sample and an adoption sample to address the following aims: (1) Evaluate the association between parental depressive symptoms and offspring internalizing/externalizing problems after accounting for familial confounds. (2) Identify the role of genetic transmission in these associations. (3) Evaluate these associations within a longitudinal framework allowing for the testing of bidirectional effects. Our CoT analysis will assist us in our first two aims, and our adoption sample in all three research aims.
Our CoT analysis will contribute to the literature by evaluating associations between parent depression and adolescent offspring internalizing/externalizing problems using a design employed for this purpose only twice previously. Our adoption study will examine longitudinal associations between birth parent depressive symptoms, adoptive-parent depressive symptoms, and offspring internalizing/externalizing problems in middle childhood. While previous studies have been cross-sectional or have focussed only on the impact of parental depression on offspring outcomes, our adoption study will be the first to examine bidirectional relationships between adoptive-parent depressive symptoms and offspring internalizing/externalizing problems. Further, it will be the first study examining this association in middle childhood.
Ethical standards
The authors assert that all procedures contributing to this work comply with the ethical standards of the relevant national and institutional committees on human experimentation and with the Helsinki Declaration of 1975, as revised in 2008.
Method
CoT sample
Data were drawn from the Twin Offspring Study of Sweden (TOSS) and included 387 MZ and 489 DZ twin families (a same-sex twin pair, each with a spouse and an adolescent child). Twin offspring were selected so that cousins were the same sex and did not differ in age by more than 4 years. Thirty-seven percent of twin pairs and 52% of offspring were male. Mean ages were 15.7 years for offspring (s.d. = 2.4, range 11–22), 44.8 years for twins (s.d. = 4.9, range 32–60), and 45.5 years for spouses (s.d. = 5.4, range = 25–65). Further information on TOSS is given elsewhere (Neiderhiser & Lichtenstein, Reference Neiderhiser and Lichtenstein2008).
Adoption sample
Data were drawn from the EGDS, a sample of adopted children, and their birth parents and adoptive parents. EGDS comprises two cohorts followed from 3 months postpartum. We focus on the first (older) cohort, comprising 361 adoptive families: 361 sets of adoptive parents and 359 birth mothers. The mean age of birth mothers at the child's birth was 24.1 years (s.d. = 5.9, range = 14–43). The mean age of adoptive parents was 37.8 years (s.d. = 5.5, range = 22–54). In this study we focus primarily on assessments conducted when children were aged 4.5, 6 and 7 years. Forty-three per cent of the children were female. Further details on EGDS can be found elsewhere (Leve et al. Reference Leve, Neiderhiser, Shaw, Ganiban, Natsuaki and Reiss2013).
Measures – CoT sample
Parental depressive symptoms were measured in twins using the self-report Center for Epidemiological Studies Depression scale (CES-D; Gatz et al. Reference Gatz, Johansson, Pedersen, Berg and Reynolds1993), comprising 20 self-report items assessing the severity of depressive symptoms in the last week. The CES-D correlates with other depression measures and performs well in identifying depressed patients (Weissman et al. Reference Weissman, Sholomskas, Pottenger, Prusoff and Locke1977). It has good internal consistency, and is a reliable measure for detecting depressive symptoms in Swedish samples (Gatz et al. Reference Gatz, Johansson, Pedersen, Berg and Reynolds1993).
Offspring internalizing and externalizing problems were measured using the Child Behaviour Checklist (CBCL; Achenbach & Rescorla, Reference Achenbach and Rescorla2001). Twins, spouses and offspring reported on offspring internalizing and externalizing problems, assessed with 32 and 30 items, respectively. Composite scales were created, taking the average of all reports. The CBCL is widely used and has been reported as a valid and reliable tool for assessing adolescent psychopathology (Dutra et al. Reference Dutra, Campbell and Westen2004). Correlations between reporters ranged from 0.30 to 0.45 for offspring internalizing, and from 0.36 to 0.57 for offspring externalizing.
Measures – adoption sample
Parental depressive symptoms were measured using the self-report Beck Depression Inventory (BDI; Beck et al. Reference Beck, Steer and Garbin1996). Items assessed the severity of depressive symptoms in the previous week. Adoptive parents were assessed when children were aged 4.5, 6, and 7 years. The depression score of the primary caregiver was used. In most cases this was the adoptive mother. In same-sex families the parent who adopted the role of primary caregiver was used (6 families of 2 adoptive mothers and 10 families of 2 adoptive fathers). Birth-mother symptoms were assessed at 4 months, 18 months and 4.5 years post-adoption. In EGDS 20 BDI items were used (suicidal ideation was excluded from the questionnaire). The BDI is widely reported as a valid and reliable measure of depressive symptoms (Beck et al. Reference Beck, Steer and Brown1988).
Child internalizing and externalizing problems were measured using the CBCL. Both adoptive parents were asked to report on their child's behaviour when children were aged 4.5, 6, and 7 years. Composite scales were created at each age, taking the average of parent reports. Correlations between parents ranged from 0.40 to 0.45 for offspring internalizing, and from 0.47 to 0.57 for externalizing.
Control variables: Obstetric complications and adoption openness (contact between birth and adoptive families) were controlled for. Obstetric complications were measured using a pregnancy screener that birth mothers completed (assessing weight change, blood pressure, vitamin use, medications, laboratory tests, due/birth dates, timing/frequency of doctor visits, and symptoms of illnesses), and a pregnancy history calendar, wherein mothers reported perinatal substance use and psychopathology. Further details can be found elsewhere (Marceau et al. Reference Marceau, Hajal, Leve, Reiss, Shaw, Ganiban, Mayes and Neiderhiser2013). Adoption openness was measured by asking adoptive parents how much contact they had with birth parents at every wave of data collection. Birth parents were asked to report how much contact they had with adoptive parents at 4 months, 18 months and 4.5 years postpartum. The measure used in the present study is a composite of birth-mother and adoptive-parent reports of adoption openness at each wave (see Ge et al. Reference Ge, Natsuaki, Martin, Leve, Neiderhiser, Shaw, Villareal, Reid, Scaramella and Reiss2008).
Analyses: children of twins
Prior to analyses residuals were taken to control for twin sex and ageFootnote †. All variables were log-transformed to correct for skew. We fitted structural equation models using maximum likelihood estimation in the programme OpenMx (Boker et al. Reference Boker, Neale, Maes, Wilde, Spiegel, Brick, Spies, Estabrook, Kenny, Bates, Mehta and Fox2011). Models allowed us to quantify the effects of additive genetic (A), common environmental (C; non-genetic effects that make members of a family similar to one another) and non-shared environmental effects (E; environmental effects that make members of a family different to one another) on parental depressive symptoms. By comparing the magnitude of MZ twin correlations (attributable to A + C) to DZ twin correlations [attributable to (0.5 × A) + C], genetic and environmental influences were estimated. Comparing MZ and DZ avuncular correlations to parent–child correlations allowed for estimation of genetic and environmental intergenerational pathways. Comparing correlations between cousins from MZ and DZ families allowed for the estimation of genetic and non-shared environmental effects on offspring phenotype. The full CoT model is included in the Supplementary online material (Supplementary Fig. S1), along with matrix specifications (Supplementary Table S1). Of note, and in contrast to more typical multivariate twin models, our model includes a direct ‘phenotypic transmission’ pathway between parental phenotype and offspring phenotype. This path is designed to capture covariance between parent and offspring phenotypes not attributable solely to direct genetic transmission – effects associated with exposure to parental phenotype. Purely genetic transmission is captured by a path linking parent and child genetic factors. The significance of pathways were tested by creating sub-models in which paths were fixed to zero. χ 2 difference tests and Akaike's Information Criterion were used to assess whether sub-models yielded a significantly worse fit to the data than the full model.
Analyses: adoption sample
Using maximum likelihood estimation in Mplus 6.11 (Muthén & Muthén, Reference Muthén and Muthén1998–2010) we fitted an autoregressive cross-lagged model to the adoption data, with the addition of birth-mother depressive symptoms as a measure of genetic risk. This model was designed as a parsimonious method of assessing whether longitudinal phenotypic associations between adoptive parent and child remained after accounting for ‘genetic risk’. We followed Pemberton et al. (Reference Pemberton, Neiderhiser, Leve, Natsuaki, Shaw, Reiss and Ge2010) by defining birth-mother depressive symptoms as a latent variable comprising self-reported depressive symptoms at 4, 18, and 54 months postpartum. The resultant variable indexes stable depression, and reduces measurement error by minimizing the impact of time-point specific variations in symptoms. It is thus well suited to index genetic risk for depression since prior research shows that persistent, stable depression is under greater genetic influence than less stable or temporary forms of depression (Kendler et al. Reference Kendler, Thornton and Gardner2001). This latent variable was included as a predictor of child internalizing/externalizing problems at all ages.
Obstetric complications were included as a covariate in associations between birth-mother depressive symptoms and child internalizing/externalizing symptoms at age 4.5 years (the earliest measurement in our model). Openness of adoption was included as a covariate in associations involving child internalizing/externalizing symptoms.
Results: children of twins
Descriptive statistics and phenotypic correlations are included in the Supplementary material (Supplementary Tables S2 and S3).
Twin correlations
Comparing twin correlations in Table 1, the MZ twin correlation of 0.35 was larger than the DZ twin correlation of 0.14, indicating that parental depression was heritable. Point estimates suggested correlations between cousins in MZ families were larger than those in DZ families, although confidence intervals suggested differences were not statistically significant (0.16 v. 0.10 and 0.22 v. 0.14, for internalizing and externalizing, respectively). Parent–child correlations were higher than MZ avuncular correlations (0.26 v. 0.07 and 0.18 v. 0.09), suggesting intergenerational associations may not be wholly attributable to familial confounds. Most avuncular correlations were non-significant, and no MZ avuncular correlations were significantly larger than DZ avuncular correlations, suggesting genetic factors do not play a primary role in explaining intergenerational correlations.
Table 1. Intraclass correlations for the relationships between twin parental depression and offspring internalizing/externalizing problems (95% confidence intervalsin parentheses)
Twin 1 is the parent of child 1. Twin 2 is the parent of child 2. Correlations are taken from constrained saturated models in which variances and means were constrained across twin order and zygosity and parent–child covariances were constrained across zygosity.
Structural equation modelling
Path diagrams are presented in Fig. 1. Model fitting (Supplementary Table S4) showed that dropping the phenotypic association from the internalizing model (p: estimated at 0.28 in Fig. 1) significantly worsened model fit, but dropping the genetic pathway (A1’: estimated at 0.00) did not. Similarly, dropping the phenotypic association from the externalizing model (p: estimated at 0.15) led to a significantly worse model fit, but eliminating the genetic pathway (A1’: estimated at 0.02) did not. These findings demonstrate that associations between parental depression and offspring internalizing and externalizing problems were not attributable to genetic transmission.
Fig. 1. Path diagrams showing the relationship between parental depression and offspring internalizing/externalizing problems. Path estimates are taken from the full (unconstrained) models in which all parameters are freely estimated. A1, Additive genetic effects on parental depression; C1, shared-environmental effects on parental depression; E1, non-shared environmental effects on parental depression; A1′, genetic effects common to parental depression and offspring phenotype; A2, familial effects specific to offspring phenotype; E2, non-shared environmental effects on offspring phenotype. Note that the pathway between A1 and A1′ is fixed to 0.50 because parents and children share 50% of their genome.
Results: adoption study
Descriptive statistics and correlations are included in Supplementary Tables S5 and S6.
Structural equation modelling
Models for internalizing (Fig. 2) and externalizing problems (Fig. 3) tell similar stories. Cross-lagged paths indicated that child internalizing and externalizing problems were predictive of later adoptive-parent depressive symptoms (range 0.09–0.13). Of note, paths from adoptive-parent depressive symptoms to subsequent child internalizing and externalizing problems were not significant (range 0.01–0.08). Birth-mother depressive symptoms predicted child internalizing at age 7 years but not at 4.5 or 6 years, and predicted child externalizing at 4.5 and 7 years, but not at 6 years. Although it may seem unusual that birth-mother depressive symptoms predicted externalizing problems at 4.5 and 7 years but not 6 years, we attribute this to the short time lapse between 4.5 and 6 years, combined with the high continuity in externalizing between waves (0.72). Together this probably left little genetic variance in externalizing behaviour to account for at age 6 years above that already accounted for at age 4.5 years.
Fig. 2. Structural equation model showing the relationship between parental depressive symptoms and offspring internalizing problems in the EGDS sample (95% confidence intervals). Parameter estimates are all standardized. Significant pathways are represented with solid lines, non-significant pathways are dashed. This is the full (unconstrained) model in which all parameters are freely estimated. AP, Adoptive parent. For adoptive-parent depressive symptoms at 6 years, R 2 = 0.45, p < 0.001. For adoptive-parent depressive symptoms at 7 years, R 2 = 0.41, p < 0.001. For child internalizing at 6 years R 2 = 0.52, p < 0.001. For child internalizing at 7 years R 2 = 0.34, p < 0.001.
Fig. 3. Structural equation model showing the relationship between parental depressive symtoms and offspring externalizing problems in the EGDS sample (95% confidence intervals). Parameter estimates are all standardized. Significant pathways are represented with solid lines, non-significant pathways are dashed. This is the full (unconstrained) model in which all parameters are freely estimated. AP, Adoptive parent. For adoptive-parent depressive symptoms at 6 years, R 2 = 0.45, p < 0.001. For adoptive-parent depressive symptoms at 7 years, R 2 = 0.41, p < 0.001. For child externalizing at 6 years R 2 = 0.53, p < 0.001. For child externalizing at 7 years R 2 = 0.53, p < 0.001.
Discussion
We set out to establish the nature of the association between parental depressive symptoms and offspring internalizing and externalizing problems using the TOSS CoT sample, and the EGDS adoption sample. Results from both analyses demonstrated that phenotypic associations remained after accounting for genetic transmission, suggesting associations result from environmental influences. These findings align with previous similar studies (Cadoret et al. Reference Cadoret, Ogorman, Heywood and Troughton1985; Tully et al. Reference Tully, Iacono and McGue2008; Natsuaki et al. Reference Natsuaki, Ge, Leve, Neiderhiser, Shaw, Conger, Scaramella, Reid and Reiss2010; Pemberton et al. Reference Pemberton, Neiderhiser, Leve, Natsuaki, Shaw, Reiss and Ge2010; Silberg et al. Reference Silberg, Maes and Eaves2010; Singh et al. Reference Singh, D'Onofrio, Slutske, Turkheimer, Emery, Harden, Heath, Madden, Statham and Martin2011; Marmorstein et al. Reference Marmorstein, Iacono and McGue2012; Kerr et al. Reference Kerr, Leve, Harold, Natsuaki, Neiderhiser, Shaw and Reiss2013; Laurent et al. Reference Laurent, Leve, Neiderhiser, Natsuaki, Shaw, Fisher, Marceau, Harold and Reiss2013a , Reference Laurent, Leve, Neiderhiser, Natsuaki, Shaw, Harold and Reiss b ). We are aware of only one published finding to contradict ours – a CoT analysis, wherein it was found that the association between parental depression and conduct problems was not significant after accounting for genetic transmission (Singh et al. Reference Singh, D'Onofrio, Slutske, Turkheimer, Emery, Harden, Heath, Madden, Statham and Martin2011). This particular study used diagnostic criteria to define depression and conduct problems in young adults, where our study, and those of others whose findings concur with our own, assessed continuous measures of subclinical symptoms in adolescents. It is possible that the difference in measurement approach or age explains the difference in results.
Where previous genetically informative studies have not considered the possibility of child-to-parent effects, our analysis of the EGDS dataset was designed to assess bidirectional effects between parent and child. Intriguingly, models indicated that child internalizing and externalizing symptoms predicted subsequent parental depressive symptoms, but the reverse was not true. This is what would be expected if children's emotional and behavioural difficulties affect the emotional wellbeing of their parents. It should be noted, however, that confidence intervals on the parent–child and child–parent paths overlapped. As such we cannot conclude that child-to-parent effects are significantly larger than parent-to-child effects. Regardless, ours is the first genetically informed study to show that child internalizing and externalizing problems prospectively predict parental depressive symptoms.
Genetic associations between parental depressive symptoms and offspring internalizing and externalizing problems
While results from both studies demonstrated that associations between parental depressive symptoms and offspring internalizing/externalizing persisted after controlling for genetic transmission, findings relating to genetic transmission were not entirely consistent across samples. TOSS results support previous CoT studies in finding no genetic transmission between parental depressive symptoms and offspring internalizing (Silberg et al. Reference Silberg, Maes and Eaves2010; Singh et al. Reference Singh, D'Onofrio, Slutske, Turkheimer, Emery, Harden, Heath, Madden, Statham and Martin2011). Our EGDS findings are the first of their kind however, showing a significant effect of birth-parent depressive symptoms on offspring internalizing problems. Previous CoT and adoption studies have found no such association. It is possible that age explains our findings, or more precisely, the age gap between parent and child. Birth-mother depression in EGDS was defined as depressive symptoms in young adults (birth mothers), and this predicted offspring internalizing problems at age 7 years. In previous studies, age gaps were larger, with parents being in middle age and offspring in adolescence (Cadoret et al. Reference Cadoret, Ogorman, Heywood and Troughton1985; Tully et al. Reference Tully, Iacono and McGue2008; Silberg et al. Reference Silberg, Maes and Eaves2010; Singh et al. Reference Singh, D'Onofrio, Slutske, Turkheimer, Emery, Harden, Heath, Madden, Statham and Martin2011; Marmorstein et al. Reference Marmorstein, Iacono and McGue2012), or birth parents being young adults and their children in early childhood (Natsuaki et al. Reference Natsuaki, Ge, Leve, Neiderhiser, Shaw, Conger, Scaramella, Reid and Reiss2010; Pemberton et al. Reference Pemberton, Neiderhiser, Leve, Natsuaki, Shaw, Reiss and Ge2010; Kerr et al. Reference Kerr, Leve, Harold, Natsuaki, Neiderhiser, Shaw and Reiss2013). It is known that genetic factors involved in depression are not static and change over time, so it may be that genetic overlap between parent and offspring emotional problems decreases as the age gap between them increases.
On a related note, the lack of evidence in support of genetic transmission in our TOSS analysis and in other CoT analyses should not be viewed as contradicting the notion that familial transmission of depression is at least partially genetic. Rather, we show that the association between concurrent parental depressive symptoms and adolescent offspring internalizing problems is not attributable to genetic transmission. The absence of such associations may be indicative of different genes influencing depression during different developmental periods. If we were to measure depressive symptoms at the same age in parents and offspring, we would expect evidence for genetic transmission.
Ours is the first CoT study to report no genetic transmission between parental depressive symptoms and adolescent externalizing problems. However, our EGDS findings conform to previous CoT (Silberg et al. Reference Silberg, Maes and Eaves2010; Singh et al. Reference Singh, D'Onofrio, Slutske, Turkheimer, Emery, Harden, Heath, Madden, Statham and Martin2011) and adoption (Pemberton et al. Reference Pemberton, Neiderhiser, Leve, Natsuaki, Shaw, Reiss and Ge2010; Kerr et al. Reference Kerr, Leve, Harold, Natsuaki, Neiderhiser, Shaw and Reiss2013) studies in demonstrating an association between genetic risk for depressive symptoms and offspring externalizing problems. It is intriguing that the evidence for genetic transmission from parent depressive symptoms to offspring externalizing problems is more consistent across studies than that from parent depression to offspring internalizing problems. It is possible that childhood externalizing problems may have more genes in common with adult depression than does childhood internalizing problems. Symptomatically depression would appear to have more overlap with internalizing than externalizing so this finding is not intuitive. It would be interesting to examine whether child externalizing problems and adult depression have greater genetic overlap than child internalizing and adult depression in longitudinal twin studies.
The genetic overlap between child externalizing problems and adult depressive symptoms is compatible with the inclusion of ‘irritable mood’ as a core symptom of DSM-5 depression (APA, 2013) only when the onset is during childhood. Of note, irritability, one facet of externalizing behaviour, has been shown to have greater phenotypic and genetic links with depression than with delinquency (Stringaris et al. Reference Stringaris, Zavos, Leibenluft, Maughan and Eley2012). Thus it may be that during childhood, genetic risk for depression manifests as irritability, a trait more often captured by measures of externalizing than internalizing problems.
Comparing the results from EGDS and TOSS
We found evidence for genetic effects in EGDS but not TOSS. One reason for this could be that mothers whose children are adopted at birth are at greater genetic risk for depression than are twin mothers, and therefore pass on greater genetic risk to their children. Another possibility relates to our use of a latent factor to index genetic risk in EGDS. This factor was defined by multiple assessments carried out over several years, so captured variance common to depressive symptoms at 3 time points. Persistent depression has been reported as being under greater genetic influence than single episodes of depression (Kendler et al. Reference Kendler, Thornton and Gardner2001), and longitudinal twin studies demonstrate that continuity in depression is predominantly attributable to genetic effects (Lau & Eley, Reference Lau and Eley2006; Kendler et al. Reference Kendler, Gardner and Lichtenstein2008). As such, our use of longitudinal data to create a latent proxy measure of genetic risk in EGDS may explain why we find genetic effects in EGDS but not TOSS.
It is worth noting that alternative approaches to the TOSS CoT data may have detected genetic overlap between parental depression and offspring internalizing/externalizing problems. For example, we also explored applying standard multivariate twin models to our data (i.e. a Cholesky decomposition and a correlated factors solution) wherein offspring internalizing/externalizing problems were modelled as a parental phenotype. These models suggested that some of the correlation could be attributed to genetic overlap. As such, our findings do not necessarily mean that there is no overlap in the genes involved in parent depressive symptoms and offspring internalizing/externalizing problems. Rather, they suggest that the intergenerational association is best conceptualised as environmental in nature (i.e. attributable to exposure).
Limitations
In the present study we used CoT and adoption data to complement one another; each approach providing distinct techniques through which to assess the nature of associations between parental depression and offspring internalizing/externalizing problems (Rutter et al. Reference Rutter, Pickles, Murray and Eaves2001). However, despite assessing the same phenotypes using overlapping measures, TOSS and EGDS were not perfectly complementary to one another. Specifically, children were in different developmental periods in each (childhood in EGDS, adolescence in TOSS). However, in spite of this, the two studies concur in many of their findings. Specifically, phenotypic associations persisted after controlling for genetic overlap. As a result there does not appear to be a reason to assume that the age difference between TOSS and EGDS offspring has unduly impacted our results.
Our analyses were underpowered to explore sex differences. However, some researchers have previously reported that sex differences may exist in the association between parental depression and offspring psychopathology (e.g. Davies & Windle, Reference Davies and Windle1997). In the future it is hoped that genetically informative studies will be large enough to have the power to examine sex differences in pathways from paternal/maternal depression to male/female offspring.
In TOSS, both parents and the child reported on adolescent externalizing and internalizing problems, but in EGDS only parents reported on child adjustment. While the use of self-report is not practicable in child samples, it is possible that the different approaches to measurement may have contributed to differences in results. Another limitation is that both studies involved the use of normative samples, so it is unclear to what extent findings generalise to clinical populations.
Conclusions
Limitations notwithstanding, results of the present study suggest that phenotypic relationships between parental depression and offspring emotional and behavioural problems are significant above and beyond potential genetic confounding. Where prior studies have not tested for bidirectional effects, our EGDS findings suggest that the residual phenotypic association (that remaining after accounting for genetic overlap) should not be assumed to be inherently parent-to-child in nature, but involves child-to-parent effects. If the association between parental depression and offspring emotional and behavioural problems is not wholly genetic in nature, and is bidirectional, then interventions aimed at reducing parental depression and/or child emotional and behavioural problems would do well to target the parent–child dyad together.
Supplementary material
For supplementary material accompanying this paper visit http://dx.doi.org/10.1017/S0033291715000501.
Acknowledgements
The first author is supported by the Leverhulme Trust (RPG-210). The Twin and Offspring Study in Sweden was supported by Grant R01MH54610 from the National Institute of Mental Health. The Early Growth and Development Study was supported by grants R01HD042608 from NICHD, NIDA, and OBSSR, NIH, U.S. PHS, by R01 DA020585 from NIDA, NIMH, and OBSSR, NIH, U.S. PHS, by grant R01 MH092118 from NIMH, NIH, U.S. PHS. We are very grateful to the adoption agencies and families who participated in the study. We thank Xiaojia Ge, John Reid, Rand Conger, Jody Ganiban, and Laura Scaramella for their scientific contributions to the larger study. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Eunice Kennedy Shriver National Institute of Child Health and Human Development or the National Institutes of Health.
Declaration of Interest
None.
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