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A novel shiga toxigenic E. coli sequence type harbouring multidrug resistance isolated from raw milk

Published online by Cambridge University Press:  18 November 2024

Anjali Karunatt Nampoothiri
Affiliation:
Department of Microbiology, Sree Sankara College, Kalady, India
Sudha Kalyanikkutty*
Affiliation:
Department of Biotechnology, St. Peter's College, Kolenchery, India
*
Corresponding author: Sudha Kalyanikkutty; Email: [email protected]

Abstract

The objective was to examine the prevalence of extended-spectrum β-lactam (ESBL) resistance among Shiga toxigenic Escherichia coli (STEC) isolated from raw milk. The findings from disc diffusion analysis and polymerase chain reaction revealed a high occurrence of ESBL resistance, specifically to penicillins, cephalosporins, and carbapenems, in isolated STEC strains. Additionally, a distinct sequence type of STEC was also identified in milk through comparative analysis.

Type
Research Article
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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References

Aslantaş, Ö, Yılmaz, , Büyükaltay, K and Kocabağ, HD (2022) Whole-genome sequencing of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae from retail raw milk. International Journal of Dairy Technology 75, 7782.CrossRefGoogle Scholar
Center for Disease Control and Prevention (2023) Shiga toxin-producing E. coli (STEC).Google Scholar
CLSI (2021) Performance Standards for Antimicrobial Susceptibility Testing 31st ed. CLSI supplement M100.Google Scholar
Joseph, J and Kalyanikutty, S (2022) Occurrence of multiple drug-resistant Shiga toxigenic Escherichia coli in raw milk samples collected from retail outlets in South India. Journal of Food Science and Technology 59, 21502159.CrossRefGoogle ScholarPubMed
Joseph, J, Sankarshanan, M and Kalyanikutty, S (2022) Repetitive extragenic palindromic and enterobacterial repetitive intergenic consensus sequence-based typing of shiga toxin-producing Escherichia coli from bovine samples. Journal of Food Safety 42, e12977.CrossRefGoogle Scholar
Liu, H, Meng, L, Dong, L, Zhang, Y, Wang, J and Zheng, N (2021) Prevalence, antimicrobial susceptibility, and molecular characterization of Escherichia coli isolated from raw milk in dairy herds in northern China. Frontiers in Microbiology 12, 730656.CrossRefGoogle ScholarPubMed
Sethulekshmi, C, Latha, C and Anu, CJ (2018) Occurrence and quantification of Shiga toxin-producing Escherichia coli from food matrices. Veterinary World 11, 104111.CrossRefGoogle ScholarPubMed
Tajima, F (1989) Statistical methods to test for nucleotide mutation hypothesis by DNA polymorphism. Genetics 123, 585595.CrossRefGoogle Scholar
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