Asthma is a chronic inflammatory disorder affecting over 300 million people worldwide1. With an aetiology remaining elusive^{Reference Asher1}, and the importance of diet and lifestyle on chronic diseases^{Reference Kim2}, research is focused on understanding the link between immunomodulatory nutrients present in diet with asthma risk^{Reference Kim2}. The n-3 polyunsaturated fatty acids (PUFAs), commonly found in fish, are well known for their anti-inflammatory properties4 and previous observational studies have shown mothers taking n-3 PUFAs supplements during pregnancy had reduced likelihood of their children developing asthma^{Reference Bisgaard3,} Reference Dunstan⁴. There is limited research studying the habitual intake of n-3 PUFAs during pregnancy and asthma rates; therefore, this present study aims to investigate the associations between n-3 PUFAs consumed as part of the habitual diet during pregnancy with incidence of asthma at 7 years of age in a high fish-eating population. Pregnant women were recruited between 2008–2011 in the high fish-eating population of the Republic of Seychelles. Blood samples were collected from mothers at 28-week gestation and cord samples were collected at delivery. Maternal and cord PUFAs were measured using gas chromatography-mass spectrometry. In 2015, when children (N = 1098) were approximately 7 years of age (mean 7.36 years), the parent(s) were asked to complete a questionnaire, validated by the International Study of Asthma and Allergies in Childhood Questionnaires (ISAAC) which determined asthma-related outcomes. Logistic regression models investigated the associations between maternal and cord PUFAs with a reported asthma outcome. From the completed questionnaires (N = 1098), 1 in 10 children (63 males; 48 females) were found to be asthmatic. In quartile analysis (lowest quartile vs highest quartile), alpha-linolenic acid (ALA; C18:3n-3) during pregnancy was significantly associated with decreased incidence of asthma in children [OR 2.771 (95% CI 1.464-5.245) p < 0.01]. There were no significant associations between any of the other maternal or cord PUFAs (linoleic acid [LA], arachidonic acid [AA], eicosapentaenoic acid [EPA], docosahexaenoic acid [DHA]) and incidence of asthma. Maternal status of alpha-linolenic acid, an n-3 PUFA, during pregnancy may decrease the incidence of asthma. This highlights the importance of intake of n-3 PUFAs during pregnancy and the potential risks associated with chronic disease; however, future analysis is needed to understand the benefits of n-3 PUFAs from fish during pregnancy, as well as eicosanoid production, n-3 PUFAs derived compounds.