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Identification of Oryza glaberrima as a potential resistance source to rice root-knot nematode, Meloidogyne graminicola

Published online by Cambridge University Press:  29 November 2023

Gurwinder Kaur ,
Narpinderjeet Kaur Dhillon ,
Gurpreet Singh ,
Yogesh Vikal Open the ORCID record for Yogesh Vikal [Opens in a new window] ,
Navneet Kaur ,
Adeshpal Singh Gill ,
Kumari Neelam Open the ORCID record for Kumari Neelam [Opens in a new window] ,
Jagveer Singh ,
Dharminder Bhatia  and
Gurjit Singh Mangat
Gurwinder Kaur
Affiliation:
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India
Narpinderjeet Kaur Dhillon
Affiliation:
Department of Plant Pathology, Punjab Agricultural University, Ludhiana, India
Gurpreet Singh
Affiliation:
Department of Plant Pathology, Punjab Agricultural University, Ludhiana, India
Yogesh Vikal*
Affiliation:
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India
Navneet Kaur
Affiliation:
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India
Adeshpal Singh Gill
Affiliation:
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India
Kumari Neelam
Affiliation:
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India
Jagveer Singh
Affiliation:
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India
Dharminder Bhatia
Affiliation:
Department of Plant Breeding & Genetics, Punjab Agricultural University, Ludhiana, India
Gurjit Singh Mangat
Affiliation:
Department of Plant Breeding & Genetics, Punjab Agricultural University, Ludhiana, India
*
Corresponding author: Yogesh Vikal; Email: yvikal-soab@pau.edu
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Abstract

The root-knot nematodes (RKN) (Meloidogyne graminicola) are a devastating threat to rice worldwide. The cultivated germplasm is either susceptible or moderately resistant to rice RKN. Therefore, there is a need to identify resistance sources against M. graminicola as an eco-friendly management strategy. The present study evaluated the host response of Oryza sativa genotypes comprising basmati, non-basmati improved varieties, their advanced breeding lines (83) and Oryza glaberrima accessions (42) against M. graminicola in the nematode-infested plot for two consecutive years. All O. sativa genotypes exhibited susceptible responses, while O. glaberrima accessions showed variable levels of resistance. Three of the O. glaberrima accessions (IRGC102196, IRGC102538 and IRGC102557) were highly resistant. M. graminicola significantly affected plant growth parameters in susceptible genotypes compared to resistant O. glaberrima accessions. The results were supported by histopathological studies that showed apparent giant cell formation in PR121 while penetration and development of M. graminicola juveniles were low in the O. glaberrima acc. IRGC102196. In silico analysis indicated that none of the reported nematode resistance genes from different crops had homology with the rice genome. The two anti-nematode genes (Oryzacystatin-I and Oryzacystatin-II) from O. sativa japonica revealed homology with O. sativa cv. PR121 and O. glaberrima acc. IRGC102206. Comparative analysis of these genes between PR121 and O. glaberrima acc. IRGC102206 resulted in the identification of SNPs/InDels that could be associated with nematode resistance. The identified SNPs/InDels could be validated, and further molecular studies are needed to provide insights into the resistance mechanism against rice RKN.

Keywords

in silicoMeloidogyne graminicolaOryza glaberrimaOryza sativaresistanceSEM
Type
Research Article
Information
Plant Genetic Resources , Volume 21 , Issue 5 , October 2023 , pp. 432 - 442
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany

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