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To investigate the effect of ALA and CLA on glucose tolerance and adipose tissue inflammation in the ApoE.3L.CETP transgenic mouse model

Published online by Cambridge University Press:  29 September 2017

Y.M. Lenighan ,
M.E. O'Reilly ,
S. Kajani ,
S.B. Curley ,
R. Bruen ,
F.C. McGillicuddy  and
H.M. Roche
Y.M. Lenighan
Affiliation:
Nutrigenomics Research Group, Conway Institute of Biomedical and Biomolecular Research and Institute of Food and Health, University College Dublin, Belfield, Dublin, Ireland
M.E. O'Reilly
Affiliation:
Nutrigenomics Research Group, Conway Institute of Biomedical and Biomolecular Research and Institute of Food and Health, University College Dublin, Belfield, Dublin, Ireland
S. Kajani
Affiliation:
Diabetes Complications Research Centre, UCD Conway Institute of Biomedical and Biomolecular Research and School of Medicine, University College Dublin, Belfield, Dublin, Ireland
S.B. Curley
Affiliation:
Diabetes Complications Research Centre, UCD Conway Institute of Biomedical and Biomolecular Research and School of Medicine, University College Dublin, Belfield, Dublin, Ireland
R. Bruen
Affiliation:
Diabetes Complications Research Centre, UCD Conway Institute of Biomedical and Biomolecular Research and School of Medicine, University College Dublin, Belfield, Dublin, Ireland
F.C. McGillicuddy
Affiliation:
Diabetes Complications Research Centre, UCD Conway Institute of Biomedical and Biomolecular Research and School of Medicine, University College Dublin, Belfield, Dublin, Ireland
H.M. Roche
Affiliation:
Nutrigenomics Research Group, Conway Institute of Biomedical and Biomolecular Research and Institute of Food and Health, University College Dublin, Belfield, Dublin, Ireland
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Abstract

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Proceedings of the Nutrition Society , Volume 76 , Issue OCE3: Irish Section Meeting, 21–23 June 2017, What governs what we eat? , 2017 , E71
Copyright
Copyright © The Authors 2017 

This abstract was awarded the student prize for best poster original communication.

Alpha-linolenic acid (ALA) and conjugated linoleic acid (CLA) have been associated with anti-inflammatory effects( Reference Zhao, Etherton and Martin 1 , Reference Loscher, Draper and Leavy 2 ). A diet high in saturated fatty acids (SFA) induces insulin resistance and a sub-acute, chronic pro-inflammatory state in adipose and the liver( Reference Finucane, Lyons and Murphy 3 ). This study addressed the hypothesis that supplementing a high SFA-fat diet (HFD) with ALA or CLA (HFD) may ameliorate HFD insulin resistance and attenuate adipose tissue inflammation. ApoE.3L.CETP transgenic mice were fed either a low fat diet (LFD, 10 % kcal from fat), HFD-SFA (45 % kcal SFA), HFD-ALA (42 % kcal SFA, 3 % kcal ALA) or HFD-CLA diet (42 % kcal SFA, 3 % kcal ALA) for 12 weeks. Following the dietary intervention, the metabolic phenotype was characterised using glucose tolerance tests (GTT) and insulin tolerance tests (ITT). Epididymal adipose tissue was harvested to isolate the stromal vascular fraction (SVF) cellular fraction which is enriched in immune cells and stimulated with 1 ng/ml IL-1β+TNFα for 18 hours. Resultant adipose IL-6 secretion was measured by ELISA. Prism version 5 (GraphPad Software Inc.) was used for statistical analysis. One- and two-way ANOVA tested for differences between diets in the GTT and adipose IL-6 secretion.

Counterintuitively, Figure 1 demonstrates significantly greater glucose intolerance after the HFD-ALA diet, compared to the HFD-CLA (P < 0·01). As anticipated the LFD was associated with adequate glucose tolerance compared to all of other HFD groups (P < 0·05) (Figure 1). Adipose SVF IL-6 secretion, in response to the combined IL-1β+ TNFα challenge, demonstrated that the IL-6 response was significantly lower in LFD than all the HFD groups (P < 0·05) (Figure 2.). Fatty acid composition within the different HFDs had no significant differential effect on adipose SVF IL-6. In conclusion, this work demonstrates that substituting dietary SFA with ALA promoted glucose intolerance, and replacement with either ALA or CLA failed to attenuate HFD-induced inflammation in these overweight human transgenic mice. Further analysis is on-going with a view to understanding why HFDs enriched with ALA are potentially more adverse than those enriched in SFA.

Fig. 1. GTT.

Fig. 2. SVF IL-6 Secretion.

This project was funded by the Irish Department of Agriculture, Food and the Marine, ‘Healthy Beef’ Programme (13/F/514).

References

1. Zhao, G, Etherton, TD, Martin, KR et al. (2005) Biochem. Biophys. Res. Commun. 336(3) 909–917.CrossRefGoogle Scholar
2. Loscher, CE, Draper, E, Leavy, O et al. (2005) J. Immunol. 175(8) 4990–4998.CrossRefGoogle Scholar
3. Finucane, OM, Lyons, CL, Murphy, AM et al. (2015) Diabetes 64, 2116–2128.CrossRefGoogle Scholar
Figure 0

Fig. 1. GTT.

Figure 1

Fig. 2. SVF IL-6 Secretion.