Implications
Dehulling is a relevant process to reduce the fiber content of rapeseed meal and to concentrate protein and energy fraction. However it seems to have a low impact on phosphorus (P) and calcium (Ca) digestibility, whereas it improves phosphorus equivalency of microbial phytase. This implies that phosphorus equivalencies need to be adapted according to the type of rapeseed meal used and to the fiber level.
Introduction
The inclusion of co-products, such as 00-rapeseed meal (RSM), in pig diets increased over the last decade because of its higher availability and its low glucosinolates and erucic acid content. Nevertheless, RSM is characterized by a relatively high dietary fiber and phytate content (0.50%) which are considered as anti-nutritional factors. Procedures, such as dehulling the seed, are of great interest to reduce the dietary fiber fraction which is mainly located in the hulls (Carré et al., Reference Carré, Quinsac, Citeau and Fine2015). However, the impact of dietary fiber of RSM on P digestibility remains unknown whereas dietary provision of P for pigs is still an environmental and economic challenge. Previous studies focused on measuring the apparent, true or standardized digestibility of P in diets with RSM, but did not investigate the specific effect of RSM fiber on digestive process (Adhikari et al., Reference Adhikari, Heo and Nyachoti2015; Shi et al., Reference Shi, He, Yu, Mao, Zheng, Huang and Chen2015).
According to the literature, the effects of dietary fiber on P digestibility in pigs are not consistent and depend strongly on the source and the inclusion level (Metzler and Monsenthin, Reference Metzler and Monsenthin2008). Dietary fiber can affect digestive process in the upper gastrointestinal tract because of their physico-chemical properties (Jha and Berrosco, Reference Jha and Berrosco2015), and consequently phytate degradation by microbial phytase (Kemme et al., Reference Kemme, Schlemmer, Mroz and Jongbloed2006; Dersjant-Li et al., Reference Dersjant-Li, Awati, Schulze and Partridge2015). The decrease of the pH between the terminal ileum and the cecum (from 7.0 to 6.0) due to microbial breakdown of fiber may improve the solubility of minerals, thereby increasing their absorption (Scholz-Arhens and Schrezenmeir, Reference Scholz-Arhens and Schrezenmeir2007). In addition, dietary fiber could affect Ca digestibility (Nortey et al., Reference Nortey, Patience, Sands, Trottier and Zijlstra2008) modifying in this way Ca and P balance and consequently bone mineralization. In this regard, we hypothesized that (1) dehulling the seed could improve nutrient digestibility by reducing the fiber matrix effect, (2) the hull content could lead to fermentations in the lower intestine enhancing mineral solubility and (3) the response to microbial phytase might be affected by RSM inclusion.
Therefore, the aim of the present study was to investigate the effects of dietary fiber content of RSM on P and Ca digestibility as well as on bone mineralization in growing pigs fed diets without or with microbial phytase, and to evaluate their impacts on P and Ca solubility in relation to the pH, and their recovery throughout the gastrointestinal tract.
Material and methods
The experimental protocol was approved by the Regional Ethics Committee on animal experimentation (Rennes, France) and the French Ministry of Higher Education and Research (Paris, France; authorization: 02402.03).
Raw materials and experimental diets
The RSM used was a 00-rapeseed meal. From a single batch of seed, whole RSM and dehulled RSM were processed at the CREOL pilot plant (Pessac, France; Supplementary Table S1). The dehulling of seed was performed before oil extraction and hulls were collected. Extraction was done using hexane.
The eight experimental diets were based on 67% corn and 10% soybean meal. Phosphorus and Ca were marginally deficient in diets without microbial phytase according to the French recommendations for 40 kg pigs (Jondreville and Dourmad, Reference Jondreville and Dourmad2005). The dietary fiber content of the diets was modulated by adding RSM. The first diet (wRSM) included 15% whole RSM to adjust the protein concentration to 15% (Table 1). The second diet (dRSM) included 10.5% dehulled RSM to provide similar amounts of protein to wRSM. The third and fourth diets (dRSMh1 and dRSMh2, respectively) included 10.5% dRSM and 4.5% or 9.0% hulls, respectively. Diets were supplemented with 0 or 500 phytase units (FTU) microbial phytase per kg (Natuphos® from Aspergillus niger; BASF SE, Ludwigshafen, Germany). The level of apparent digestible P (Sauvant et al., Reference Sauvant, Perez and Tran2004) was fixed at 0.16% in diets without microbial phytase and at 0.24% in diets supplemented with microbial phytase, in accordance with the French recommendations, considering an equivalency of 0.8 g/kg of apparent digestible P with 500 FTU microbial phytase/kg (Jondreville and Dourmad, Reference Jondreville and Dourmad2005). The Ca : apparent digestible P ratio was adjusted to 2.6 using calcium carbonate. Titanium dioxide (TiO2, 0.30%) was added as an indigestible marker. The diets were offered to pigs as pellets.
Table 1 Ingredients and chemical composition of experimental diets (as-fed basis)
wRSM=whole rapeseed meal; dRSM=dehulled rapeseed meal; dRSMh1=dehulled rapeseed meal with 4.5% rapeseed hulls; dRSMh2=dehulled rapeseed meal with 9% rapeseed hulls (−=without microbial phytase and + = with 500 FTU/kg); BUF=buffering capacity to pH 3.
1 Analyzed values (as described in ‘Material and methods’ section).
2 Calculated values.
3 Apparent digestible phosphorus (Sauvant et al., Reference Sauvant, Perez and Tran2004).
Animals and experimental procedures
The experiment included 48 castrated male Piétrain×(Landrace×Large White) pigs weighing 36.1±0.4 kg. Animals were housed in crates equipped with a slatted floor with a tray underneath that allowed separation of urines and feces collection. From day 0 to day 7, the pigs received a standard diet covering all their nutrient requirements (9.7 MJ net energy and 18% CP). At day 8, pigs were assigned to one of the eight experimental diets (six pigs/diet) in a complete randomized block design (six blocks of eight treatments). The pigs were offered an equal quantity of feed daily distributed in two equivalent meals corresponding to 4% of their BW. Daily feed intake was recorded individually during the experimental period. Collection period started at day 14 and ended at day 23 after an overnight fast to ensure the emptying of the intestine. Feces were collected at meal times (twice a day) and pooled by pig. At the end of the collection period, feces were mixed thoroughly with water, sampled, weighed and freeze-dried. Acidified urine (1% of the total, with 3 ml of 10% sulfuric acid/l of urine) was also collected individually each day and stored at −20°C. Pigs were weighed at day 0, day 8, day 14 and day 23. Room temperature was maintained at 20±1°C. At the end of the experiment, individual pigs received half of their daily feed allowance in the morning and the other half 2 h 30 min before slaughter. They were anesthetized by electro-immobilization and slaughtered by exsanguination. The viscera were immediately removed and samples of digesta were collected from stomach, duodenum, jejunum (from the end of duodenum to the beginning of ileum), proximal ileum (1 m before distal ileum), distal ileum (80 cm anterior to the ileocecal valve), cecum and middle of the colon, homogenized and stored at −80°C. A sample of digesta from the cecum and the distal ileum was freeze-dried for 48 h and then ground. Femurs were also collected and stored at −20°C.
Analyses
All samples were analyzed in duplicate. The crude fiber content was measured with the Weende method (NF V03 040), and NDF, ADF and ADL were obtained according to Van Soest et al. (Reference Van Soest, Robertson and Lewis1991). Soluble and insoluble fibers were determined according to Prosky method (AOAC 991.42 and 993.19). The initial pH and the buffering capacity to pH 3 were measured according to Lawlor et al. (Reference Lawlor, Lynch, Caffrey, O’Reill and Connell2005). Dry matter of diets, feces and digestive content of ileum and cecum, and P, Ca and TiO2 analysis were performed as previously explained by Rousseau et al. (Reference Rousseau, Valable, Létourneau-Montminy, Même, Godet, Magnin, Nys, Duclos and Narcy2016). Phytase activity was measured according to Engelen et al. (Reference Engelen, van der Heeft, Randsdorp and Smits1994). Levels of Ca and P content were measured in diets, feces and urine to determine apparent total tract digestibility (ATTD) and apparent retention (AR). Levels of P, Ca and TiO2 were measured in diets and digesta to determine Ca and P recovery. The soluble Ca and inorganic P (iP) levels were determined in the stomach and cecal supernatant fractions, after centrifuging the samples at 23 000 g for 10 min at 4°C. Inorganic P was analyzed using a kit (Kit Phosphore UV 61571; bioMérieux, 69280 Marcy l'Etoile, France). Nitrogen (NF EN ISO 5983-2) and fat (NF ISO 6492) were determined in diets. The pH was measured on all digestive contents with a pH meter (InLab Solid Pro-ISM with SevenGo DuoTM SG23; Mettler Toledo, Greifensee, Switzerland). Right femurs were broken and then defatted using ether, dried at 103°C for 18 h and finally ashed in a muffle furnace at 600°C for 16 h. The left femurs were analyzed using computed tomography (Siemens Emotion Duo, Erlangen, Germany). Bone densitometry (BD) was determined using X-ray absorbance: mineral density was proportional to grayscale value in Hounsfield units (HU), from 250 to 1500 HU.
Calculations and statistical analysis
Ileal and cecal recovery were calculated using the following equation:
$${\rm Recovery}{\,\equals\,}\left( {X_{{{\rm diet}}} /X_{{{\rm digesta}}} } \right){\times}\left( {{\rm TiO}_{{{\rm 2digesta}}} /{\rm TiO}_{{{\rm 2diet}}} } \right)$$
Where X is the amount of P or Ca in the diet or in the digesta (mg/g DM) and TiO2 the amount of TiO2 in the diet or in the digesta (mg/g DM).
The P equivalency values were calculated using the ATTD of P and the dietary P content following the method of Kerr et al. (Reference Kerr, Weber, Miller and Southern2010), and the analyzed values of phytase activity.
All data were analyzed using the MIXED procedure of SAS (SAS Institute Inc., Cary, NC, USA) as appropriate for a randomized complete block design. Crates location was the blocking factor and pigs were the experimental unit. The model included diets as fixed effect and block as random effect. Orthogonal polynomial contrasts were used to separate treatment means. Contrasts were chosen to study the effects of (1) microbial phytase (linear (Lin), mean of diets without phytase v. mean of diets with phytase), (2) dehulled rapeseed without microbial phytase (Lin, wRSM− v. dRSM−) and (3) with microbial phytase (Lin, wRSM+ v. dRSM+), (4) the hull content without microbial phytase (Lin, quadratic (Qua), dRSM− v. dRSMh1− v. dRSMh2−) and (5) with microbial phytase (Lin, Qua, dRSM+ v. dRSMh1+ v. dRSMh2+). Differences were considered significant at P<0.05 and to have a tendency at 0.05⩽P<0.10. Relationships between the pH and the mineral solubility in the stomach and in the cecum were quantified using regression analysis with the REG procedure of SAS. The relation between the iP solubility in the stomach and BD was estimated using a quadratic regression model as well as broken-line and curvilinear-plateau regression models. Estimates were obtained using REG and NLIN procedures of SAS (Supplementary Figure S1).
Results
Nutritional values of experimental diets
The P and Ca concentrations analyzed in the experimental diets were in accordance with the expected values (Table 1). Analyzed phytase activity was 650, 450, 870 and 630 FTU/kg in wRSM+, dRSM+, dRSMh1+ and dRSMh2+ diets, respectively, and less than the detection limit of 100 FTU/kg in the diets without microbial phytase. As expected, NDF, ADF, ADL, insoluble and soluble fiber contents decreased when including dRSM (7.8%, 2.9%, <0.5%, 1.2% and 10.7%, respectively). The dRSMh1 diet contained almost the same level of fibers as wRSM while dRSMh2 presented the highest level of fibers (13.1%, 7.3%, 2.8%, 1.8% and 15.5%, respectively).
Growth performance and bone mineralization
There was no significant effect of microbial phytase, dehulled seed and hull content on BW at day 8, day 23 or on average daily gain between day 8 and day 23 (Table 2). The inclusion of hulls without microbial phytase influenced the feed conversion ratio (Qua; P=0.037) and the inclusion of hulls with microbial phytase increased the average daily feed intake (P=0.017). Microbial phytase increased femur ash weight (P<0.001) and content (P=0.005), and bone density (P<0.001).
Table 2 Growth performance and femurs characteristics in pigs fed experimental diets namely whole rapeseed meal (wRSM), dehulled RSM (dRSM) or dRSM supplemented with 4.5% or 9.0% rapeseed hulls (dRSMh1 and dRSMh2, respectively)Footnote 1
ADG=average daily gain; ADFI=average daily feed intake; FCR=feed conversion ratio; BD=bone density: area greater than 250 HU; Lin=linear; Qua=quadratic.
1 n=6 pigs per diet.
2 Contrast analysis: linear effect of microbial phytase; linear effect of dehulled rapeseed seed without and with microbial phytase; linear and quadratic effects of the inclusion of hulls without and with microbial phytase.
3 Microbial phytase supplementation: −=without microbial phytase and +=with 500 FTU/kg.
Phosphorus and calcium balance
Microbial phytase decreased P fecal excretion (−25%, P<0.001; Table 3), increased P urinary excretion (+30%, P=0.020), ATTD of P (+18.4 points; P<0.001), AR of P (+17.6 points; P<0.001), Ca fecal excretion (+33%, P<0.001) and AR of Ca (+6.7 points; P=0.011). With microbial phytase, the inclusion of hulls affected P urinary excretion (+36%; Qua, P=0.040). The P equivalency values were 0.48, 0.69, 0.88 and 0.84 g of apparent digestible P/kg of diet for wRSM, dRSM, dRSMh1 and dRSMh2, respectively.
Table 3 Intake, fecal excretion, urinary excretion, apparent total tract digestibility (ATTD), apparent retention (AR) in pigs fed experimental diets namely whole rapeseed meal (wRSM), dehulled RSM (dRSM) or dRSM supplemented with 4.5% or 9.0% rapeseed hulls (dRSMh1 and dRSMh2, respectively)Footnote 1
Lin=linear; Qua=quadratic.
1 n=6 pigs per diet.
2 Contrast analysis: linear effect of microbial phytase; linear effect of dehulled rapeseed seed without and with microbial phytase; linear and quadratic effects of the inclusion of hulls without and with microbial phytase.
3 Microbial phytase supplementation: −=without microbial phytase and +=with 500 FTU/kg.
pH of digestive contents and stomach solubility, ileal recovery and cecal solubility and recovery of phosphorus and calcium
Microbial phytase increased duodenum (P=0.031) and colon pH (P=0.017; Table 4) except in the duodenum of pigs fed the wRSM diet. The dRSM− diet increased jejunum (P=0.006) and cecum pH (P<0.001) compared with wRSM−. Without microbial phytase, the inclusion of hulls decreased cecum pH (Lin, P=0.006). Total and soluble P and Ca in the stomach and cecum are presented in the Supplementary Table S2. Microbial phytase increased iP solubility in the stomach (P<0.001; Table 4) and decreased iP solubility (P=0.008) and P recovery in the cecum (P<0.001). Without microbial phytase, the inclusion of hulls increased iP solubility (Lin and Qua, P<0.001) and recovery of Ca in the cecum (Lin, P=0.017 and Qua, P=0.033). With microbial phytase, the inclusion of hulls increased iP solubility (Lin, P=0.026 and Qua, P=0.014) and decreased Ca solubility (Lin, P=0.001 and Qua, P=0.032).
Table 4 Phosphorus and calcium solubility in the stomach and the cecum, recovery in the ileum and cecum and pH of digesta in different segments of the digestive tract in pigs fed experimental diets namely whole rapeseed meal (wRSM), dehulled RSM (dRSM) or dRSM supplemented with 4.5% or 9.0% rapeseed hulls (dRSMh1 and dRSMh2, respectively, DM basis)Footnote 1
Lin=linear; Qua=quadratic.
1 n=6 pigs per diet.
2 Contrast analysis: linear effect of microbial phytase; linear effect of dehulled rapeseed seed without and with microbial phytase; linear and quadratic effects of the inclusion of hulls without and with microbial phytase.
3 Microbial phytase supplementation: −=without microbial phytase and +=with 500 FTU/kg.
Regression analysis
With microbial phytase, soluble iP was negatively correlated with the pH of the stomach content (R 2=0.31, P=0.009; Figure 1a) but no effect was observed without microbial phytase. Soluble Ca was negatively correlated with the pH of the stomach content without microbial phytase (R 2=0.53, P<0.001; Figure 1b) and with MP (R 2=0.85, P<0.001). Soluble iP in the cecum was negatively correlated with pH without (R 2=0.40, P<0.001; Figure 2) and with microbial phytase (R 2=0.24, P=0.026).
Figure 1 Relation between stomach pH and stomach solubility of P (a) and Ca (b) in pigs fed diets without microbial phytase (□) or with microbial phytase (●). (a) Without microbial phytase: y=0.139+0.027x, R 2=0.02, P=0.52, n=24; with microbial phytase: y=2.790−0.396x, R 2=0.31, P=0.009, n=20. (b) Without microbial phytase: y=1.223−0.188x, R 2=0.53, P<0.001, n=24; with microbial phytase: y=2.098−0.329x, R 2=0.85, P<0.001, n=21.
Figure 2 Relation between cecum pH and cecum solubility of P in pigs fed diets without microbial phytase (□) and with microbial phytase (●). Without microbial phytase: y=2.178−0.296x, R 2=0.40, P<0.001, n=24; with microbial phytase: y=1.337−0.183x, R 2=0.24, P=0.026, n=21.
Discussion
Using dRSM instead of wRSM or adding hulls did not affect the ATTD or AR of P and Ca. However, the P equivalency value was twofold lower with the wRSM diet than expected (0.48 v. 0.83 g/kg; Kerr et al., Reference Kerr, Weber, Miller and Southern2010). Moreover, the solubility of iP in the stomach tended to be enhanced with dehulled seeds in diets supplemented with microbial phytase. These results suggest that dehulling the seed enhances the action of microbial phytase which takes place mainly in the stomach. Phytate is distributed throughout the kernel in sub-cellular inclusions known as globoids (Singh, Reference Singh2008). Removing hulls from the seed may reduce binding and physical entrapment of phytate, improving in this way the accessibility to the enzyme (Baye et al., Reference Baye, Guyot and Mouquet-River2015). This hypothesis is confirmed by the fact that the positive effect is remaining with the dRSMh1+ diet although it almost contains the same level of fibers as wRSM+. In addition, neither dehulled seeds nor the inclusion of hulls significantly affected the absorption of P and Ca in the small intestine. There is some evidence that fiber can modulate the absorption of minerals from the upper gastrointestinal tract (Metzler and Monsenthin, Reference Metzler and Monsenthin2008). The estimated content in hemicelluloses fractions was increased by almost 20% between dRSM and wRSM or dRSMh2 diets. The ability of hemicelluloses to bind Ca is particularly high at neutral pH, especially compared with lignin (Claye et al., Reference Claye, Idouraine and Weber1998). The level of inclusion of hulls in the present experiment might have been too low to induce significant effects in the small intestine on mineral availability. The hull content of the diet did not affect the cecal recovery of P. Nevertheless, increasing the level of hulls reduced the pH of the cecal content (6.14 v. 5.84) in pigs fed diets without microbial phytase and slightly improved the solubility of iP in the cecal content. Fiber from hulls, although mostly insoluble, may have been fermented and thus lowered the pH. The higher acidity produced through fermentation resulted in greater solubility and availability of P. Indeed, both soluble and insoluble dietary fiber can be degraded by intestinal bacteria, but insoluble fiber is less easily, rapidly and completely fermented than soluble fiber (Bach Knudsen and Hansen, Reference Bach Knudsen and Hansen1991). The effects of fiber on mineral absorption in the hindgut are controversial. The source and the inclusion level of dietary fiber have a considerable effect on P absorption or secretion in the large intestine as a result of the different bacteria P needs for fermentation (Metzler and Monsenthin, Reference Metzler and Monsenthin2008). For instance, an increase in the cellulose content of the diet from 3% to 9% was reported to negatively affect the absorption of P from the large intestine (Partridge, Reference Partridge1978b). In contrast, some studies using various types of fiber have suggested greater P absorption (Den Hartog et al., Reference Den Hartog, Huisman, Thielen, Van Schayk, Boer and Van Weeden1988; Heijnen and Beynen, Reference Heijnen and Beynen1998). The cecal recovery of Ca was higher when the inclusion level of hulls was increased (from 0.53 to 0.61 on average for dRSM and dRSMh2, respectively). Moreover, although low, the solubility of Ca was reduced when the hull content in diets supplemented with microbial phytase was increased. These results can be explained by the higher Ca requirement to buffer the excess of acidity in the cecum with progressing fermentation as illustrated by Metzler-Zebeli et al. (Reference Metzler-Zebeli, Hooda, Mosenthin, Gänzle and Zijlstra2010) who reported a negative relationship between volatile fatty acids in feces and net post-ileal Ca absorption.
Microbial phytase significantly improved the ATTD of P from 26.1% to 44.6%. A similar improvement in the ATTD of P from 28% to 56% was previously reported when phytase was added (863 FTU/kg) to a diet containing 13.2% canola meal (Akinmusire and Adeola, Reference Akinmusire and Adeola2009). Microbial phytase supplementation led to an increase of Ca intake as dietary Ca level was adjusted using calcium carbonate in order to keep an equal Ca : apparent digestible P ratio. However, microbial phytase had no impact on ATTD of Ca. This result is particularly surprising since microbial phytase is well known to improve Ca digestibility in canola meal as shown by González-Vega et al. (Reference González-Vega, Walk, Liu and Stein2013). However, in our experiment, calcium carbonate was added and its level was twice as high in diets supplemented with microbial phytase. According to González-Vega et al. (Reference González-Vega, Walk and Stein2015a), Ca from calcium carbonate is more easily bound to phytate, less soluble or less digestible than other sources which could explain the lack of significant effect of microbial phytase on ATTD of Ca. Calcium AR was significantly improved in pigs fed diets supplemented with microbial phytase (45.2% v. 38.5%) with a reduction in urinary Ca (from 21% to 12% of the absorbed Ca). Microbial phytase also caused a large improvement of bone ash content, as previously reported in finishing pigs fed diets supplemented with 1000 FTU of microbial phytase/kg which led to an 8 percentage units increase compared with unsupplemented diets (Varley et al., Reference Varley, Lynch, Callan and O’Doherty2010). These observations are related to the greater availability of P for bone mineralization and illustrate the close relationship between P and Ca at the metabolic level. Both minerals are deposited together in the skeleton to form hydroxyapatite crystals and the element in excess compared with the other is excreted in the urine (Létourneau-Montminy et al., Reference Létourneau-Montminy, Narcy, Magnin, Sauvant, Bernier, Pomar and Jondreville2010).
There was a strong negative relationship between stomach pH and iP solubility in pigs fed diets supplemented with microbial phytase. The presence of feed in the stomach induces distension, with a subsequent increase in intra-gastric pressure that stimulates acid secretion (Yen, Reference Yen2001). The relatively acidic pH (5.45) is particularly favorable to phytate solubility and phytase activity (Yi and Kornegay, Reference Yi and Kornegay1996). In consequence, a large amount of phosphate was released in this compartment. There was also a negative relationship between stomach pH and Ca solubility in pigs fed diets without or with microbial phytase. Maintenance of a low stomach pH may thus prevent the formation of Ca-phosphate complexes. Moreover, the higher level of provision of calcium carbonate in the diets supplemented with microbial phytase led to a higher buffering capacity of the feed (129 v. 159). This caused a slight increase in pH of the stomach content (5.36 v. 5.54) which remained sufficiently low for optimal activity of microbial phytase which is between pH 2.5 and 5.5 (Dersjant-Li et al., Reference Dersjant-Li, Awati, Schulze and Partridge2015). Solubilization and hydrolysis processes occurring in the stomach proved to be limiting steps in P utilization (Kemme et al., Reference Kemme, Schlemmer, Mroz and Jongbloed2006). This was supported by the positive relationship between iP solubility in the stomach and bone density as the skeleton is the main storage site of P in the body (c.f. Supplementary Material). However, it is worthy to note that pH values in the stomach are higher than those generally found in the literature and must be taken with care. The δ between sampling and pH measurement may explain these results as a consequence of acid secretion disruption. Microbial phytase did not have any effect on the ileal recovery of P while a significant decrease in cecal recovery occurred. These results suggest that part of the absorption of P occurred in the cecum, which potentiates the effects of microbial phytase through the absorption of P released by enzymes in the proximal part of the digestive tract. The small intestine is the major site of P absorption (Breves and Schröder, Reference Breves and Schröder1991). The role of the large intestine in the regulation of P absorption is more controversial. Some authors have reported secretion of P in the large intestine (Partridge, Reference Partridge1978a; Partridge et al., Reference Partridge, Simons and Bergner1986), whereas others have reported significant absorption in both the cecum and the proximal colon (Liu et al., Reference Liu, Bollinger, Ledoux and Venum2000). A significant decrease in the pH of the digestive content from the ileum to the cecum occurred (from 7.6 to 5.9), presumably due to volatile fatty acids production resulting from fiber fermentation. Results of previous studies showed a decrease in pH of 6.6 to 7.2 near the ileocecal junction to 5.7 to 6.8 in the cecum of pigs fed various wheat- or oat-based diets (Bach Knudsen and Hansen, Reference Bach Knudsen and Hansen1991). Moreover, a strong negative relationship between the pH of the digesta of the cecum and the solubility of iP was found. The low pH in the large intestine might have resulted in the improvement of the solubility of Ca and phosphate, with a subsequent increase in their absorption (González-Vega et al., Reference González-Vega, Walk and Stein2015b). It is of note that the solubility of both P and Ca in the cecum was extremely low (around 2.4%). This could be attributed to the buffering functions of Ca and phosphate in order to compensate for the low pH. Indeed, Ca and phosphate have a major role for the buffering capacity throughout the intestinal lumen, forming insoluble complexes at pH values above 6 (Lawlor et al., Reference Lawlor, Lynch, Caffrey, O’Reill and Connell2005; Selle et al., Reference Selle, Cowieson and Ravindran2009). The higher Ca intake in pigs fed diets with phytase caused an increase in the flow of Ca entering the large intestine, which might explain the higher pH of the digesta of the colon observed with microbial phytase.
In conclusion, the present findings indicate that dietary fiber content of RSM does not have a strong effect on digestive processes in the proximal part of the gastrointestinal tract of pigs. However, dehulling of RSM improved the availability of phytate and the phosphate released in the stomach. This study showed that the large intestine has a significant role in the absorption of P and potentiates the effect of microbial phytase due to its low pH. Dietary fiber from rapeseed induces acidification of the cecal content, thereby improving iP solubility, which may improve its absorption.
Acknowledgments
The authors are grateful to MiXscience, Terres Inovia and Terres Univia for their financial support, M. Alix, A. Chauvin, F. Le Gouevec, C. Jaeger, R. Janvier, J. Liger and V. Piedvache for the animal care, Y. Jaguelin and A. Starck for their help with laboratory analysis and D. Gaudré for his expert advice on bone density results.
Supplementary Material
To view supplementary material for this article, please visit https://doi.org/10.1017/S1751731117001343
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