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Zebrafish (Danio rerio) as a possible bioindicator of epigenetic factors present in drinking water that may affect reproductive function: is chorion an issue?

Published online by Cambridge University Press:  05 March 2014

M. Martinez-Sales ,
F. García-Ximénez  and
FJ. Espinós
M. Martinez-Sales*
Affiliation:
Aquaculture and Environmental Research Group (ACUMA), Universidad Politécnica de Valencia, Camino de Vera 14, 46022, Valencia, Spain.
F. García-Ximénez
Affiliation:
Aquaculture and Environmental Research Group (ACUMA), Universidad Politécnica de Valencia, Camino de Vera 14, 46022, Valencia, Spain.
FJ. Espinós
Affiliation:
Aquaculture and Environmental Research Group (ACUMA), Universidad Politécnica de Valencia, Camino de Vera 14, 46022, Valencia, Spain.
*
All correspondence to: M. Martinez-Sales. Aquaculture and Environmental Research Group (ACUMA), Universidad Politécnica de Valencia, Camino de Vera 14, 46022, Valencia, Spain. Tel: +34 963879433. e-mail: [email protected]
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Summary

Emerging organic contaminants have been monitored in stream waters, raw and finished waters and wastewater effluents. Most of these contaminants, such as epigenetic substances, have been detected at very low levels. Unfortunately, their complete monitoring and/or removal are very difficult, given the increasing presence of new contaminants and due to analytical and economic considerations. For this reason, bioindicators are used as an alternative to monitor their presence. To this end, zebrafish is being used to assess certain contaminants in water quality studies.

As our long-term aim is to determine if zebrafish (Danio rerio) can be used to detect environmental epigenetic factors in drinking waters with effects on human reproduction, an initial question is whether the chorion could interfere with the possible action of epigenetic factors in two reproductive events: genital ridge formation and migration of the primordial germ cells (PGCs) to these genital ridges.

In the first experiment, we attempted to partially degrade the chorion of mid blastula transition (MBT) embryos with pronase, with acceptable survival rates at 5 days post fertilisation (dpf), with the group exposed for 15 min giving the best survival results. As denuded early embryos require a specific culture medium, in the next experiment embryo survival was evaluated when they were cultured up to 5 dpf in drinking waters from six different sources. Results showed a negative effect on embryo survival at 5 dpf from several waters but not in others, thus distorting the survival outcomes. These results suggest using embryos with the chorion intact from the outset when drinking waters from different sources are to be tested.

Keywords

BioindicatorChorionDrinking waterPronaseZebrafish
Type
Research Article
Information
Zygote , Volume 23 , Issue 3 , June 2015 , pp. 447 - 452
Copyright
Copyright © Cambridge University Press 2014 

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