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Xepac protein and IP3/Ca2+ pathway implication during Xenopus laevis vitellogenesis

Published online by Cambridge University Press:  26 July 2013

María de los Angeles Serrano ,
Melchor Emilio Luque  and
Sara Serafina Sánchez
María de los Angeles Serrano
Affiliation:
Instituto Superior de Investigaciones Biológicas (CONICET), Universidad Nacional de Tucumán, Departamento de Biología del Desarrollo, San Miguel de Tucumán, Argentina.
Melchor Emilio Luque
Affiliation:
Instituto Superior de Investigaciones Biológicas (CONICET), Universidad Nacional de Tucumán, Departamento de Biología del Desarrollo, San Miguel de Tucumán, Argentina.
Sara Serafina Sánchez*
Affiliation:
Instituto Superior de Investigaciones Biológicas (CONICET), Universidad Nacional de Tucumán, Departamento de Biología del Desarrollo, Chacabuco 461, T4000ILI San Miguel de Tucumán, Argentina.
*
All correspondence to: Sara Serafina Sánchez. Instituto Superior de Investigaciones Biológicas (CONICET), Universidad Nacional de Tucumán, Departamento de Biología del Desarrollo, Chacabuco 461, T4000ILI San Miguel de Tucumán, Argentina. Tel: +54 381 4107214. Fax: +54 381 4247752. e-mail: [email protected]
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Summary

The objective of this study was to elucidate the signalling pathways initiated by cAMP once inside the Xenopus laevis oocyte, where it triggers and maintains vitellogenin endocytic uptake. Our results showed the presence of Xepac transcripts at all stages of oogenesis and we demonstrated that a cAMP analogue that exclusively activates Xepac, 8-CPT, was able to rescue the endocytic activity in oocytes with uncoupled gap junctions. Inhibition experiments for the IP3/Ca2+ signalling pathway showed either a complete inhibition or a significant reduction of the vitellogenic process. These results were confirmed with the rescue capability of the A-23187 ionophore in those oocyte batches in which the IP3/Ca2+ pathway was inhibited. Taking our findings into account, we propose that the cAMP molecule binds Xepac protein enabling it to activate the IP3/Ca2+ pathway, which is necessary to start and maintain X. laevis vitellogenin uptake.

Keywords

cAMPIP3/Ca2+VitellogenesisXenopus laevisXepac
Type
Research Article
Information
Zygote , Volume 23 , Issue 1 , February 2015 , pp. 99 - 110
Copyright
Copyright © Cambridge University Press 2013 

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