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Viability and apoptosis in spermatozoa of transgenic rabbits

Published online by Cambridge University Press:  13 December 2010

P. Chrenek ,
A.V. Makarevich  and
M. Simon
P. Chrenek*
Affiliation:
Animal Production Research Centre Nitra, 95141 Luzianky near Nitra, Slovak Republic. Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic.
A.V. Makarevich
Affiliation:
Animal Production Research Centre Nitra, Slovak Republic.
M. Simon
Affiliation:
Institute of Animal Biochemistry and Genetics, Slovak Academy Science, Ivanka pri Dunaji, Slovak Republic.
*
All correspondence to: P. Chrenek. Animal Production Research Centre Nitra, 95141 Luzianky near Nitra, Slovak Republic. Tel: + 421 37 6546 289. Fax: + 421 37 6546 285. e-mail: [email protected]
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Summary

The aim of our study was to compare the viability of sperm cells from transgenic (mWAP-hFVIII gene) or non-transgenic (normal) rabbit males as assessed by viability (SYBR-14/PI) and apoptosis (annexin V) tests. These results were evaluated using female conception rates following insemination with the respective sperm samples. No significant differences were found in concentration and motility between transgenic and non-transgenic spermatozoa. Spermatozoa from both transgenic (63.05 ± 20.05%) or non-transgenic (65.75 ± 22.15%) males, stained with SYBR-14 (green), were found to be morphologically normal. In both groups, the highest proportion of annexin V-positive sperm staining was found in the post-acrosomal part of the sperm head (8.66 and 27.53%). The percentage of sperm that stained with SYBR-14/PI or with annexin V/DAPI was correlated with liveborn in transgenic rabbits (R2 = 0.6118 and R2 = 0.2187, respectively) or non-transgenic rabbits (R2 = 0.671 and R2 = 0.3579, respectively). These data indicate that there was no difference in the viability of rabbit transgenic and non-transgenic spermatozoa when determined by both fluorescence assays.

Keywords

Annexin VRabbitSpermatozoaSYBR-14Transgenic
Type
Research Article
Information
Zygote , Volume 20 , Issue 1 , February 2012 , pp. 33 - 37
Copyright
Copyright © Cambridge University Press 2010

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