Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-11-19T11:27:07.944Z Has data issue: false hasContentIssue false
  • English
  • Français

Value of transferring embryos derived from monopronucleated (1PN) zygotes at the time of fertilization assessment

Published online by Cambridge University Press:  20 March 2020

Ming Li Open the ORCID record for Ming Li [Opens in a new window] ,
Yujiao Dang ,
Ying Wang ,
Junsheng Li  and
Ping Liu
Ming Li*
Affiliation:
Center for Reproductive Medical, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing10091, China Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing10091, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Technology, Beijing100191, China National Clinical Research Center for Obstetrics and Gynecology, Beijing10091, China
Yujiao Dang
Affiliation:
Center for Reproductive Medical, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing10091, China Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing10091, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Technology, Beijing100191, China National Clinical Research Center for Obstetrics and Gynecology, Beijing10091, China
Ying Wang
Affiliation:
Center for Reproductive Medical, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing10091, China Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing10091, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Technology, Beijing100191, China National Clinical Research Center for Obstetrics and Gynecology, Beijing10091, China
Junsheng Li
Affiliation:
Center for Reproductive Medical, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing10091, China Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing10091, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Technology, Beijing100191, China National Clinical Research Center for Obstetrics and Gynecology, Beijing10091, China
Ping Liu
Affiliation:
Center for Reproductive Medical, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing10091, China Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing10091, China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Technology, Beijing100191, China National Clinical Research Center for Obstetrics and Gynecology, Beijing10091, China
*
Author for correspondence: Ming Li, Department of Obstetrics and Gynecology, Reproductive Medical Center, Peking University Third Hospital, No. 49 North Huayuan Road, Haidian District, Beijing100191, China. Tel: +86 10 82266849. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

This paper is a retrospective analysis of the sole transfer of monopronucleated zygotes (1PN) embryos both in in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) to determine the value of transferring embryos formed from 1PN. In fresh cycles, 1PN cleavage-stage embryos (1PN cleavage fresh) were transferred. In frozen–thawed cycles, 1PN blastocyst-stage embryos (1PN blast frozen) were transferred. We used comparison groups: for fresh cycles, 2PN cleavage-stage embryos (2PN cleavage fresh) were transferred; and for frozen–thawed cycles, 2PN blastocyst-stage embryos (2PN blast frozen) were transferred. Comparison groups were matched for cycle and patient characteristics to the 1PN group. Finally, for fresh cycles, live birth rates (LBR) in the 1PN cleavage group were significantly lower than those in 2PN cleavage group, both for IVF [LBR = 7.64% vs. pregnancy rate (PR) = 22.12%, P = 0.003, respectively] and ICSI (LBR = 0% vs. LBR = 20.00%, P < 0.001, respectively). For frozen–thawed IVF cycles, the PR in the 1PN blastocyst group were comparable with those of the 2PN blastocyst group (1PN: LBR = 33.14% vs. 2PN: LBR = 37.24%, P = 0.289, respectively), while in ICSI, the PR in the 1PN blastocyst group were lower than those in the 2PN blastocyst group (LBR = 15.25% vs. LBR = 40.68%, P = 0.002, respectively). So, for IVF, blastocyst culture was capable of selecting normal 1PN embryos for transfer and achieves satisfying outcomes. However, for ICSI, blastocyst culture was not effective enough to eliminate abnormal embryos and 1PN embryo transfer needed to be treated with caution.

Keywords

1PNBlastocyst cultureEmbryo transferImplantation rateLive birth
Type
Research Article
Information
Zygote , Volume 28 , Issue 3 , June 2020 , pp. 241 - 246
Copyright
© Cambridge University Press 2020

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Azevedo, AR, Pinho, MJ, Silva, J, Sa, R, Thorsteinsdottir, S, Barros, A and Sousa, M (2014) Molecular cytogenetics of human single pronucleated zygotes. Reprod Sci 21, 1472–82.CrossRefGoogle ScholarPubMed
Barak, Y, Kogosowski, A, Goldman, S, Soffer, Y, Gonen, Y and Tesarik, J (1998) Pregnancy and birth after transfer of embryos that developed from single-nucleated zygotes obtained by injection of round spermatids into oocytes. Fertil Steril 70, 67–70.CrossRefGoogle ScholarPubMed
Bradley, CK, Traversa, MV, Hobson, N, Gee, AJ and McArthur, SJ (2017) Clinical use of monopronucleated zygotes following blastocyst culture and preimplantation genetic screening, including verification of biparental chromosome inheritance. Reprod Biomed Online 34, 567–74.CrossRefGoogle ScholarPubMed
Capalbo, A, Treff, N, Cimadomo, D, Tao, X, Ferrero, S, Vaiarelli, A, Colamaria, S, Maggiulli, R, Orlando, G, Scarica, C, Scott, R, Ubaldi, FM and Rienzi, L (2017) Abnormally fertilized oocytes can result in healthy live births: improved genetic technologies for preimplantation genetic testing can be used to rescue viable embryos in invitro fertilization cycles. Fertil Steril 108, 1007–15 e1003.CrossRefGoogle Scholar
Chen, Y, Zheng, X, Yan, J, Qiao, J and Liu, P (2013) Neonatal outcomes after the transfer of vitrified blastocysts: closed versus open vitrification system. Reprod Biol Endocrinol 11, 107.CrossRefGoogle ScholarPubMed
Dasig, D, Lyon, J, Behr, B and Milki, AA (2004) Monozygotic twin birth after the transfer of a cleavage stage embryo resulting from a single pronucleated oocyte. J Assist Reprod Genet 21, 427–9.CrossRefGoogle ScholarPubMed
Gardner, DK, Lane, M, Stevens, J, Schlenker, T and Schoolcraft, WB (2000) Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer. Fertil Steril 73, 1155–8.CrossRefGoogle ScholarPubMed
Gras, L and Trounson, AO (1999) Pregnancy and birth resulting from transfer of a blastocyst observed to have one pronucleus at the time of examination for fertilization. Hum Reprod 14, 1869–71.CrossRefGoogle ScholarPubMed
Itoi, F, Asano, Y, Shimizu, M, Honnma, H and Murata, Y (2015) Birth of nine normal healthy babies following transfer of blastocysts derived from human single-pronucleate zygotes. J Assist Reprod Genet 32, 1401–7.CrossRefGoogle ScholarPubMed
Kang, HJ and Rosenwaks, Z (2008) Triploidy--the breakdown of monogamy between sperm and egg. Int J Dev Biol 52, 449–54.CrossRefGoogle Scholar
Kroener, L, Ambartsumyan, G, Briton-Jones, C, Dumesic, D, Surrey, M, Munne, S and Hill, D (2012) The effect of timing of embryonic progression on chromosomal abnormality. Fertil Steril 98, 876–80.CrossRefGoogle ScholarPubMed
Levron, J, Munne, S, Willadsen, S, Rosenwaks, Z and Cohen, J (1995) Male and female genomes associated in a single pronucleus in human zygotes. Biol Reprod 52, 653–7.CrossRefGoogle Scholar
Liao, H, Zhang, S, Cheng, D, Ouyang, Q, Lin, G, Gu, Y, Lu, C, Gong, F and Lu, G (2009) Cytogenetic analysis of human embryos and embryonic stem cells derived from monopronuclear zygotes. J Assist Reprod Genet 26, 583–9.CrossRefGoogle ScholarPubMed
Lin, G, OuYang, Q, Zhou, X, Gu, Y, Yuan, D, Li, W, Liu, G, Liu, T and Lu, G (2007) A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following invitro fertilization procedure. Cell Res 17, 999–1007.CrossRefGoogle Scholar
Liu, N, Ma, Y, Li, R, Jin, H, Li, M, Huang, X, Feng, HL and Qiao, J (2012) Comparison of follicular fluid amphiregulin and EGF concentrations in patients undergoing IVF with different stimulation protocols. Endocrine 42, 708–16.CrossRefGoogle ScholarPubMed
Mateo, S, Parriego, M, Boada, M, Vidal, F, Coroleu, B and Veiga, A (2013) In vitro development and chromosome constitution of embryos derived from monopronucleated zygotes after intracytoplasmic sperm injection. Fertil Steril 99, 897–902 e891.CrossRefGoogle ScholarPubMed
Mateo, S, Vidal, F, Parriego, M, Rodriguez, I, Montalvo, V, Veiga, A and Boada, M (2017) Could monopronucleated ICSI zygotes be considered for transfer? Analysis through time-lapse monitoring and PGS. J Assist Reprod Genet 34, 905–11.CrossRefGoogle ScholarPubMed
Nagy, ZP, Janssenswillen, C, Janssens, R, De Vos, A, Staessen, C, Van de Velde, H and Van Steirteghem, AC (1998) Timing of oocyte activation, pronucleus formation and cleavage in humans after intracytoplasmic sperm injection (ICSI) with testicular spermatozoa and after ICSI or in-vitro fertilization on sibling oocytes with ejaculated spermatozoa. Hum Reprod 13, 1606–12.CrossRefGoogle ScholarPubMed
Palermo, G, Joris, H, Devroey, P and Van Steirteghem, AC (1992) Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 340, 17–18.CrossRefGoogle ScholarPubMed
Reichman, DE, Jackson, KV and Racowsky, C (2010) Incidence and development of zygotes exhibiting abnormal pronuclear disposition after identification of two pronuclei at the fertilization check. Fertil Steril 94, 965–70.CrossRefGoogle ScholarPubMed
Rosenbusch, B (2014) The chromosomal constitution of embryos arising from monopronuclear oocytes in programmes of assisted reproduction. Int J Reprod Med 2014, 418198.CrossRefGoogle ScholarPubMed
Staessen, C and Van Steirteghem, AC (1997) The chromosomal constitution of embryos developing from abnormally fertilized oocytes after intracytoplasmic sperm injection and conventional in-vitro fertilization. Hum Reprod 12, 321–327.CrossRefGoogle ScholarPubMed
Staessen, C, Janssenswillen, C, Devroey, P and Van Steirteghem, AC (1993) Cytogenetic and morphological observations of single pronucleated human oocytes after in-vitro fertilization. Hum Reprod 8, 221–3.CrossRefGoogle ScholarPubMed
Sultan, KM, Munne, S, Palermo, GD, Alikani, M and Cohen, J (1995) Chromosomal status of uni-pronuclear human zygotes following in-vitro fertilization and intracytoplasmic sperm injection. Hum Reprod 10, 132–136.CrossRefGoogle ScholarPubMed
van der Heijden, GW, van den Berg, IM, Baart, EB, Derijck, AA, Martini, E and de Boer, P (2009) Parental origin of chromatin in human monopronuclear zygotes revealed by asymmetric histone methylation patterns, differs between IVF and ICSI. Mol Reprod Dev 76, 101–8.CrossRefGoogle ScholarPubMed
Vega, M, Breborowicz, A, Moshier, EL, McGovern, PG and Keltz, MD (2014) Blastulation rates decline in a linear fashion from euploid to aneuploid embryos with single versus multiple chromosomal errors. Fertil Steril 102, 394–8.CrossRefGoogle Scholar
Xie, PY, Tang, Y, Hu, L, Ouyang, Q, Gu, YF, Gong, F, Leng, LZ, Zhang, SP, Xiong, B, Lu, GX and Lin, G (2018) Identification of biparental and diploid blastocysts from monopronuclear zygotes with the use of a single-nucleotide polymorphism array. Fertil Steril 110, 545–54 e545.CrossRefGoogle ScholarPubMed
Yan, J, Li, Y, Shi, Y, Feng, HL, Gao, S and Chen, ZJ (2010) Assessment of sex chromosomes of human embryos arising from monopronucleus zygotes in invitro fertilization and intracytoplasmic sperm injection cycles of Chinese women. Gynecol Obstet Invest 69, 20–3.CrossRefGoogle Scholar