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Treatment of human sperm with GYY4137 increases sperm motility and resistance to oxidative stress

Published online by Cambridge University Press:  30 October 2024

Yan Huang ,
Runxin Gan ,
Min Zhang ,
Dewei Lin ,
Yi Cheng Open the ORCID record for Yi Cheng [Opens in a new window]  and
Xinyu Guo
Yan Huang
Affiliation:
Center of Reproductive Medicine, the General Hospital of Southern Theater Command, Guangzhou, 510010 China
Runxin Gan
Affiliation:
Reproductive Medicine Center, Xiangya Hospital, Central South University, Changsha, 410008 China
Min Zhang
Affiliation:
Center of Reproductive Medicine, the General Hospital of Southern Theater Command, Guangzhou, 510010 China
Dewei Lin
Affiliation:
Center of Reproductive Medicine, the General Hospital of Southern Theater Command, Guangzhou, 510010 China
Yi Cheng*
Affiliation:
Center of Reproductive Medicine, the General Hospital of Southern Theater Command, Guangzhou, 510010 China
Xinyu Guo*
Affiliation:
Center of Reproductive Medicine, the General Hospital of Southern Theater Command, Guangzhou, 510010 China
*
Corresponding authors: Yi Cheng; Email: [email protected]; Xinyu Guo; Email: [email protected]
Corresponding authors: Yi Cheng; Email: [email protected]; Xinyu Guo; Email: [email protected]
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Summary

Hydrogen sulfide (H2S) has been shown to play a significant role in oxidative stress across various tissues and cells; however, its role in sperm function remains poorly understood. This study aimed to investigate the protective effect of GYY4137, a slow-releasing H2S compound, on sperm damage induced by H2O2. We assessed the effects of GYY4137 on motility, viability, lipid peroxidation and caspase-3 activity in human spermatozoa in vitro following oxidative damage mediated by H2O2. Spermatozoa from 25 healthy men were selected using a density gradient centrifugation method and cultured in the presence or absence of 10 μM H2O2, followed by incubation with varying concentrations of GYY4137 (0.625–2.5 μM). After 24 h of incubation, sperm motility, viability, lipid peroxidation, and caspase-3 activity were evaluated. The results indicated that H2O2 adversely affected sperm parameters, reducing motility and viability, while increasing oxidative stress, as evidenced by elevated lipid peroxidation and caspase-3 activity. GYY4137 provided dose-dependent protection against H2O2-induced oxidative stress (OS). We concluded that supplementation with GYY4137 may offer antioxidant protection during in vitro sperm preparation for assisted reproductive technology.

Keywords

H2Slipid peroxidationoxidative stresssperm motilitysperm viability
Type
Research Article
Information
Zygote , Volume 32 , Issue 5 , October 2024 , pp. 360 - 365
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Copyright
© The Author(s), 2024. Published by Cambridge University Press

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Footnotes

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These authors contributed equally to this work.

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