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Time-dependent expression of ryanodine receptors in sea urchin eggs, zygotes and early embryos

Published online by Cambridge University Press:  28 July 2021

G. Percivale ,
C. Angelini ,
C. Falugi ,
C. Picco Open the ORCID record for C. Picco [Opens in a new window]  and
G. Prestipino
G. Percivale
Affiliation:
CNR Institute of Biophysics, Via De Marini 6, 16149 Genoa, Italy
C. Angelini
Affiliation:
Laboratory of Developmental Neurobiology, Department of Earth, Environment and Life Sciences, University of Genoa, Viale Benedetto XV 5, 16132 Genoa, Italy
C. Falugi
Affiliation:
Laboratory of Developmental Neurobiology, Department of Earth, Environment and Life Sciences, University of Genoa, Viale Benedetto XV 5, 16132 Genoa, Italy
C. Picco*
Affiliation:
CNR Institute of Biophysics, Via De Marini 6, 16149 Genoa, Italy
G. Prestipino
Affiliation:
CNR Institute of Biophysics, Via De Marini 6, 16149 Genoa, Italy
*
Author for correspondence: Cristiana Picco. IBF CNR, Via de Marini 6, 16149 Genova, Italy. E-mail [email protected]
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Summary

In this work, the presence of calcium-dependent calcium channels and their receptors (RyR) has been investigated in Paracentrotus lividus eggs and early embryos, from unfertilized egg to four-blastomere stages. Electrophysiological recordings of RyR single-channel current fluctuations showed that RyRs are functional during the first developmental events with a maximum at zygote stage, c. 40 min after fertilization, corresponding to the first cleavage. The nature of vertebrate-like RyRs active at this stage was established by specific activation/blockade experiments.

Keywords

Ca2+ releaseFirst cleavageParacentrotus lividusRyanodine receptorZygote
Type
Research Article
Information
Zygote , Volume 30 , Issue 2 , April 2022 , pp. 213 - 216
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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