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Time course of meiotic spindle development in MII oocytes

Published online by Cambridge University Press:  23 June 2010

Suha Kilani ,
Simon Cooke  and
Michael Chapman
Suha Kilani*
Affiliation:
IVF Australia, 225 Maroubra Rd., Maroubra, NSW 2035, Sydney, Australia.
Simon Cooke
Affiliation:
IVF Australia, 225 Maroubra Rd., Maroubra, NSW 2035, Sydney, Australia.
Michael Chapman
Affiliation:
School of Women's and Children's Health, University of New South Wales, NSW 2052, Sydney, Australia.
*
All correspondence to: Suha Kilani. IVF Australia, 225 Maroubra Rd., Maroubra, NSW 2035, Sydney, Australia. e-mail: [email protected]
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Summary

The aim of this study was to examine changes in meiotic spindle morphology over time to potentially optimize timing for ICSI. Using polarized light microscopy, images of MII oocytes were captured after retrieval of oocytes in stimulated cycles at six time intervals in culture: 36–36.5 h, 36.5–37.0 h, 38–38.5 h, 39–39.5 h, 40–40.5 h and 40.5–41 h post hCG. Captured images were analysed for spindle presence and their retardance. Results showed that spindles were detected in 58% (45/78) of oocytes at 36–36.5 h. This percentage rose to a peak (96% vs. 58%, p < 0.001) at 39–39.5 h and stabilized between 39–40.5 h post trigger then significantly declined at 40.5–41 h post hCG (96% vs. 77%, p < 0.001). Average spindle retardance increased from 36–36.5 h (1.8 ± 0.7 nm) until it peaked at 39–40.5 h (3.8 ± 0.8 nm, p < 0.0001) and then declined significantly after 40.5–41 h (3.2 ± 0.9 nm, p = 0.0001). These results show that the meiotic spindle appearance is time dependent with the majority of oocytes having detectable spindles and highest retardance between 39–40.5 h post hCG under currently used stimulation protocol after which they start to disaggregate. 39–40.5 h post hCG may be the optimal time for ICSI.

Keywords

HumanICSIMeiotic spindle; Spindle retardanceTime course
Type
Research Article
Information
Zygote , Volume 19 , Issue 1 , February 2011 , pp. 55 - 62
Copyright
Copyright © Cambridge University Press 2010

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References

Cooke, S., Tyler, J.P.P. & Driscoll, G.L. (2003). Meiotic spindle location and identification and its effect on embryonic cleavage plane and early development. Hum. Reprod. 18, 2397–405.CrossRefGoogle ScholarPubMed
Eichenlaub-Ritter, U., Chandley, A.C. & Gosden, R.G. (1986). Alterations to the microtubular cytoskeleton and increased disorder of chromosome alignment in spontaneously ovulated mouse oocytes aged in vivo: an immunofluorescence study. Chromosoma 94, 337–45.CrossRefGoogle Scholar
Eichenlaub-Ritter, U., Stahl, A. & Luciani, J.M. (1988). The microtubular cytoskeleton and chromosomes of unfertilized human oocytes aged in vitro. Hum. Genet. 80, 259–64.CrossRefGoogle ScholarPubMed
Eichenlaub-Ritter, U., Shen, Y. & Tinneberg, H.-R. (2002). Manipulation of the oocyte: possible damage to the spindle apparatus. Reprod. Biomed. Online 5, 117–24.CrossRefGoogle Scholar
Kilani, S., Cooke, S. & Chapman, M. (2006). Does the meiotic spindle shape and retardance reflect oocyte competence? Hum. Reprod. 21 (S1), il56.Google Scholar
Kilani, S., Cooke, S., Kan, A. & Chapman, M. (2009). Are there non-invasive markers in human oocytes that can predict pregnancy outcome? Reprod. Biomed. Online. 5, 674–80.CrossRefGoogle Scholar
Liu, L., Trimarchi, J. R., Oldenbourg, R. & Keefe, D. L. (2000). Increased birefringence in the meiotic spindle provides a new marker for the onset of activation in living oocytes. Biol. Reprod. 63, 251–8.CrossRefGoogle ScholarPubMed
Montag, M., Schimming, T. & van der Ven, H. (2006). Spindle imaging in human oocytes: the impact of the meiotic cell cycle. Reprod. Biomed. Online 1, 442–6.CrossRefGoogle Scholar
Rama Raju, G.A., Prakash, G.J., Krishna, K.M. & Madan, K. (2007). Meiotic spindle and zona pellucida characteristics as predictors of embryonic development: a preliminary study using PolScope imaging. Reprod. Biomed. Online 14, 166–74.CrossRefGoogle ScholarPubMed
Rienzi, L., Ubaldi, F., Martinez, F., Iacobelli, M., Minasi, M.G., Ferrero, S., Tesarik, J. & Greco, E. (2003). Relationship between meiotic spindle location with regard to the polar body position and oocyte developmental potential after ICSI. Hum. Reprod. 18, 1289–93.CrossRefGoogle Scholar
Segers, I., Adriaenssens, T., Coucke, W., Cortvrindt, R. & Smitz, J. (2008). Timing of nuclear maturation and postovulatory aging in oocytes of in vitro-grown mouse follicles with or without oil overlay. Biol. Reprod. 78, 859–68.CrossRefGoogle ScholarPubMed
Shen, Y., Stalf, T., Mehnert, C., De Santis, L., Cino, I., Tinneberg, H.R. & Eichenlaub-Ritter, U. (2006). Light retardance by human oocyte spindle is positively related to pronuclear score after ICSI. Reprod. Biomed. Online 12, 737–51.CrossRefGoogle ScholarPubMed
Shen, Y., Betzendahl, I., Tinneberg, H.-R. & Eichenlaub-Ritter, U. (2008). Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes. Mut. Res. 651, 131–40.CrossRefGoogle ScholarPubMed
Wang, W.H., Meng, L., Hackett, R.J., Odenbourg, R. & Keefe, D.L. (2001). Limited recovery of meiotic spindles in living human oocytes after cooling-rewarming observed using polarized light microscopy. Hum. Reprod. 16, 2374–8.CrossRefGoogle ScholarPubMed
Wang, W.H., Meng, L., Hackett, R.J., Oldenbourg, R. & Keefe, D.L. (2002). Rigorous thermal control during intracytoplasmic sperm injection stabilizes the meiotic spindle and improves fertilization and pregnancy rates. Fertil. Steril. 77, 1274–7.CrossRefGoogle ScholarPubMed