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Temporal expression of pluripotency-associated transcription factors in sheep and cattle preimplantation embryos

Published online by Cambridge University Press:  23 July 2018

P.G.C. Silva ,
M.T. Moura Open the ORCID record for M.T. Moura [Opens in a new window] ,
R.L.O. Silva ,
P.S. Nascimento ,
J.B. Silva ,
J.C. Ferreira-Silva ,
L.F. Cantanhêde ,
M.S. Chaves ,
A.M. Benko-Iseppon  and
M.A.L. Oliveira
P.G.C. Silva
Affiliation:
Laboratório de Biotécnicas Reprodutivas, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Brasil
M.T. Moura*
Affiliation:
Laboratório de Biotécnicas Reprodutivas, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Brasil
R.L.O. Silva
Affiliation:
Laboratório de Genética e Biotecnologia Vegetal, Departamento de Genética, Universidade Federal de Pernambuco, Brasil
P.S. Nascimento
Affiliation:
Laboratório de Biotécnicas Reprodutivas, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Brasil
J.B. Silva
Affiliation:
Laboratório de Genética e Biotecnologia Vegetal, Departamento de Genética, Universidade Federal de Pernambuco, Brasil
J.C. Ferreira-Silva
Affiliation:
Laboratório de Biotécnicas Reprodutivas, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Brasil
L.F. Cantanhêde
Affiliation:
Laboratório de Biotécnicas Reprodutivas, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Brasil
M.S. Chaves
Affiliation:
Laboratório de Biotécnicas Reprodutivas, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Brasil
A.M. Benko-Iseppon
Affiliation:
Laboratório de Biotécnicas Reprodutivas, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Brasil
M.A.L. Oliveira
Affiliation:
Laboratório de Biotécnicas Reprodutivas, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Brasil
*
All correspondence to: M.T. Moura. Email: [email protected]
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Summary

Pluripotency-associated transcription factors (PATFs) modulate gene expression during early mammalian embryogenesis. Despite a strong understanding of PATFs during mouse embryogenesis, limited progress has been made in ruminants. This work aimed to describe the temporal expression of eight PATFs during both sheep and cattle preimplantation development. Transcript availability of PATFs was evaluated by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) in eggs, cleavage-stage embryos, morulae, and blastocysts. Transcripts of five genes were detected in all developmental stages of both species (KLF5, OCT4, RONIN, ZFP281, and ZFX). Furthermore, CMYC was detected in all cattle samples but was found from cleavage-stage onwards in sheep. In contrast, NR0B1 was detected in all sheep samples but was not detected in cattle morulae. GLIS1 displayed the most significant variation in temporal expression between species, as this PATF was only detected in cattle eggs and sheep cleavage-stage embryos and blastocysts. In silico analysis suggested that cattle and sheep PATFs share similar size, isometric point and molecular weight. A phenetic analysis showed two patterns of PATF clustering between cattle and sheep, among several mammalian species. In conclusion, the temporal expression of pluripotency-associated transcription factors differs between sheep and cattle, suggesting species-specific regulation during preimplantation development.

Keywords

EmbryogenesisIn vitro productionPluripotentPreimplantation developmentRuminant
Type
Research Article
Information
Zygote , Volume 26 , Issue 4 , August 2018 , pp. 270 - 278
Copyright
© Cambridge University Press 2018 

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