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Strain difference in the timing of meiosis resumption in mouse oocytes: involvement of a cytoplasmic factor(s) acting presumably upstream of the dephosphorylation of p34cdc2 kinase

Published online by Cambridge University Press:  26 September 2008

Zbigniew Polanński
Zbigniew Polanński*
Affiliation:
Department of Genetics Evolution, Institute of Zoology, Jagiellonian University Krabów, Poland
*
Dapartment of Genetics and Evolution, Institute of Zoology, Jagiellonian University, Ingardena 6, 30-060 Kraków, Poland. Telephone: +48-12-336377 ext 434. Fax: +48-12-343716. e-mail: [email protected].
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Summary

Oocytes from eight inbred strains of mice were screened for the timing of germinal vesicle breakdown (GVB) in vitro. This characteristic varied between strains, reaching most extreme values in oocytes from AKR and BALB/c mice (3.1 and 1.6h after release from dibutyryl cAMP block, respectively; p<0.0001). The difference between AKR and BALB/c mice was confirmed in experiments in which GVB was induced in vivo by stimulation with exogenous gonadotrophins. Analysis of the rate of GVB in hybrids obtained after fusion of nuclear and cytoplamic fragments of oocytes from both strains suggests that the factor responsible for the difference between AKR and BALB/c mice is located in the cytoplasm of the proghase oocytes. Finally, in oocytes from both strains stimulated to resume meiotic maturation with okadaic acid, an inhibitor of protein phosphatases types 1 and 2A the rate of GVB was the same (2.2h and 2.3h for AKR and BALB/c, respectively; p= 0.48). This suggests that the difference between strains is not related to the amount or quality of the pre-MPF (Maturation Promoting Factor) stored in the prophase oocyte, but to the factor(s) acting upstream of the dephosphorylation of p34cdc2. kinase in the pathway leading to pre-MPF activation.

Keywords

Cytoplasmic factor(s)InterstraindifferencesMouse oocyteTiming of GVB
Type
Article
Information
Zygote , Volume 5 , Issue 2 , May 1997 , pp. 105 - 109
Copyright
Copyright © Cambridge University Press 1997

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References

Alexandre, H., Van Cauwenberge, A., Tsukitani, Y. & Mulnard, J. (1991). Pleiotropic effect of okadaic acid on maturing mouse oocytes. Development 112, 971–80.CrossRefGoogle ScholarPubMed
Batakier, H. & Czolowska, R. (1977). Cytoplasmic control of nuclear maturation in mouse oocytes. Exp. Cell Res. 110, 466–9.Google Scholar
Bornslaeger, E.A., Mattei, P.M. & Schultz, R.M. (1986a). Involvement of cAMP-dependent protein kinase and protein phosphorylation in regulation of mouse oocyte maturation. Dcv. Biol. 114, 453–62.Google ScholarPubMed
Bornslaeger, E.A., Poueymirou, W.T., Mattei, P.M. & Schultz, R.M. (1986 b). Effects of protein kinase C activators on germinal vesicle breakdown and polar body emission of mouse oocytes. Exp. Cell Res. 165, 507–17.CrossRefGoogle ScholarPubMed
Cho, W.K., Stem, S. & Biggers, J.D. (1974). Inhibitory effect of dibutyryl cAMP on mouse oocyte maturation in vitro. J. Exp. Zool. 187, 383–6.CrossRefGoogle ScholarPubMed
Choi, T., Aoki, F., Moti, M., Yamashita, M., Nagahama, Y. & Kohmoto, K. (1991). Activation of p34cdc2 protein kinase activity in meiotic and mitotic cell cycles in mouse oocytes and embryos. Development 113, 789–95.CrossRefGoogle ScholarPubMed
Eppig, J.J. & Wigglesworth, K. (1994). Atypical maturation of oocytes of strain I/LnJ mice. Hum. Reprod. 9, 1136–42.CrossRefGoogle ScholarPubMed
Fulton, B.P.&Whittingham, D.G. (1978). Activation of mammalian oocytes by intracellular injection of calcium. Nature 273, 149–51.CrossRefGoogle ScholarPubMed
Gavin, A.C., Tsukitani, Y. & Schorderet-Slatkine, S. (1991). Induction of M-phase entry of prophase-blocked mouse oocytes through microinjection of okadaic acid, a specific phosphatase inhibitor. Exp. Cell Res. 192, 75–81.CrossRefGoogle ScholarPubMed
Hampl, A. & Eppig, J.J. (1995). Translational regulation of the gradual increase in histone H1 kinase activity in maturing mouse oocytes. Mo Reprod. Des. 40, 9–15.CrossRefGoogle ScholarPubMed
Hashimoto, N. & Kishimoto, T. (1988). Regulation of meiotic metaphase by a cytoplasmic maturation-promoting factor during mouse oocyte maturation. Des. Biol. 126, 242–52.Google ScholarPubMed
Jessus, C., Rime, H., Haccard, O., Van Lint, J., Goris, J., Merlevede, W. & Ozon, R. (1991). Tyrosine phosphorylation of p34cdc2 and p42 during meiotic maturation of Xenopus oocytes: antagonistic action of okadaic acid and 6-DMAP. Development 111, 813–20.CrossRefGoogle ScholarPubMed
King, R.W., Jackson, P.K. & Kirschner, M.W. (1994). Mitosis in transition. Cell 79, 563–71.CrossRefGoogle ScholarPubMed
Kishimoto, T. (1994). Cell reproduction: induction of M-phase events by cyclin-dependent cdc2 kinase. Int. J. Des. Biol. 38, 185–91.Google ScholarPubMed
Kubiak, J.Z., Weber, M., Geraud, G. & Maro, B. (1992). Cell cycle modification during the transitions between meiotic M-phases in mouse oocytes. J. Cell Sci. 102, 457–67.CrossRefGoogle ScholarPubMed
Maro, B., Kubiak, J.Z., Verlhac, M.H. & Winston, N.J. (1994). Interplay between the cell cycle control Machinery and the microtubule network in mouse oocytes. Semin. Des. Biol. 5, 191–8.CrossRefGoogle Scholar
Murray, A.W. (1992). Creative blocks: cell-cycle checkpoints and feedback controls. Nature 359,599–604.CrossRefGoogle ScholarPubMed
Ookata, K., Hisanaga, S., Okano, T., Tachibana, K. & Kishimoto, T. (1992). Relocation and distinct subcellular localization of p34cdc2/cyclin B complex at meiosis reinitiation in starfish oocytes. EMBO J. 11, 1763–72.CrossRefGoogle ScholarPubMed
Polanński, Z. (1986). In-vivo and in-vitro maturation rate of oocytes from two strains of mice. J. Reprod. Fertil. 78, 103–9.CrossRefGoogle Scholar
Polanński, Z. (1997). Genetic background of the differences in the timing of meiotic maturation in mouse oocytes: a study using recombinant inbred strains. J. Reprod. Fertil. 109, 109–14.CrossRefGoogle Scholar
Rime, H. & Ozon, R. (1990). Protein phosphatases are involved in the in vivo activation of histone H1 kinase in mouse oocytes. Des. Biol. 141, 115–22.Google Scholar
Tarkowski, A.K. (1977). In vitro development of haploid mouse embryos produced by bisection of one-cell fertilized eggs. J. Embnjol. Ep. Morphol. 38,187–202.Google Scholar