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Specific binding of acrosome-reaction-inducing substance to the head of starfish spermatozoa

Published online by Cambridge University Press:  26 September 2008

Akira Ushiyama ,
Takeo Araki ,
Kazuyoshi Chiba  and
Motonori Hoshi
Akira Ushiyama
Affiliation:
Department of Life Science, Tokyo Institute of Technology, Japan.
Takeo Araki
Affiliation:
Department of Life Science, Tokyo Institute of Technology, Japan.
Kazuyoshi Chiba
Affiliation:
Department of Life Science, Tokyo Institute of Technology, Japan.
Motonori Hoshi*
Affiliation:
Department of Life Science, Tokyo Institute of Technology, Japan.
*
Motonori Hoshi, Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 227, Japan. Telephone/Fax: 81-45-923-0368.
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Extract

In the starfish, spermatozoa undergo the acrosome reaction upon encountering the jelly coat of eggs. A highly sulphated glycoprotein in the jelly coat is called acrosome-reaction-inducing substance (ARIS) because it is the key signal molecule to trigger the acrosome reaction. The activity of ARIS is mainly attributed to its sulphate and saccharide residues. The extremely large molecular size and speciesspecific action of ARIS suggest the presence of a specific ARIS receptor on the sperm surface, but no experimental evidence for the receptor has been presented. We therefore measured specific binding of ARIS and its pronase digest (P-ARIS), which retains the full activity of ARIS, to homologous spermatozoa by using fluorescien-isothiocyanate-labelled ARIS and125 I-labelled P-ARIS, respectively. The spermatozoa had the ability to bind ARIS, as well as P-ARIS, specifically. The binding was species-specific, and mostly localised to the head region of spermatozoa. Scatchard plot analysis indicated the presence of one class of ARIS receptor on the surface of acrosome-intact speramatozoa. Furthermore, the specific binding of P-ARIS to the anterior region of sperm heads was microscopically confirmed by using P-ARIS conjugated to polystyrene latex beads with intense fluorescence. It is concluded that starfish spermatozoa have a specific receptor for ARIS on the surface of the anterior region of heads.

Keywords

Acrosome-reaction-inducing substanceEgg jellyGlycoproteinReceptorStarfish sperm
Type
Article
Information
Zygote , Volume 1 , Issue 2 , May 1993 , pp. 121 - 127
Copyright
Copyright © Cambridge University Press 1993

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