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Roscovitine in combination with calcium ionophore induces oocyte activation through reduction of M-phase promoting factor activity in mice

Published online by Cambridge University Press:  06 October 2011

Tomomi Iba ,
Yuya Yano ,
Mayumi Umeno ,
Kenji Hinokio ,
Akira Kuwahara ,
Minoru Irahara ,
Shuji Yamano  and
Toshiyuki Yasui
Tomomi Iba
Affiliation:
Graduate School of Health Science, The University of Tokushima Graduate School, 3–18–15 Kuramoto, Tokushima 770-8503, Japan.
Yuya Yano
Affiliation:
Department of Obstetrics and Gynecology, Tokushima University Hospital, 3-18-15 Kuramoto, Tokushima 770-8503, Japan.
Mayumi Umeno
Affiliation:
Biomedical Laboratory Sciences, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto, Tokushima 770-8503, Japan.
Kenji Hinokio
Affiliation:
Department of Obstetrics and Gynecology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto, Tokushima 770-8503, Japan.
Akira Kuwahara
Affiliation:
Department of Obstetrics and Gynecology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto, Tokushima 770-8503, Japan.
Minoru Irahara
Affiliation:
Department of Obstetrics and Gynecology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto, Tokushima 770-8503, Japan.
Shuji Yamano
Affiliation:
Department of Reproductive Technology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto, Tokushima 770-8503, Japan.
Toshiyuki Yasui*
Affiliation:
Department of Reproductive Technology, Course of Human Development, Human Development and Health Science, Institute of Health Biosciences, The University of Tokushima Graduate School, 3–18–15 Kuramoto, Tokushima 770–8503, Japan.
*
All correspondence to: Toshiyuki Yasui. Department of Reproductive Technology, Course of Human Development, Human Development and Health Science, Institute of Health Biosciences, The University of Tokushima Graduate School, 3–18–15 Kuramoto, Tokushima 770–8503, Japan. Tel: +81 88 633 9023. Fax: +81 88 631 2630. e-mail: [email protected]
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Summary

The aim of the present study was to determine oocyte activation and change in M-phase promoting factor (MPF) activity induced by treatment with calcium ionophore and roscovitine in comparison with those induced by treatment with roscovitine alone and treatment with calcium ionophore and puromycin in mice. Freshly ovulated oocytes obtained from 6–8-week-old mice were divided into five groups (no activation treatment; 5 μM calcium ionophore A23187; 50 μM roscovitine; 5 μM calcium ionophore and 10 μg/ml puromycin; and 5 μM calcium ionophore and 50 μM roscovitine) and were incubated for 6 h. Oocyte activation, assessed by morphological changes, and changes in MPF activity in the five groups at 0, 2, 4 and 6 h of incubation were examined. Activated oocytes were defined as oocytes with at least one pronucleus. Oocytes treated with roscovitine alone were not activated during the 6-h incubation period. All of the oocytes in the calcium ionophore with puromycin group and in the calcium ionophore with roscovitine group were activated. The percentage activity of MPF in oocytes treated with roscovitine alone was decreased after 2 h and increased after 4 h of incubation. The percentage activity of MPF in oocytes treated with calcium ionophore and roscovitine was significantly decreased with suppression of MPF activity being maintained for 6 h, and this change was similar to that in oocytes treated with calcium ionophore and puromycin. Roscovitine with calcium ionophore is effective for induction of oocyte activation through suppression of MPF activity in mice.

Keywords

Calcium ionophoreMPFOocyte activationRoscovitine
Type
Research Article
Information
Zygote , Volume 20 , Issue 4 , November 2012 , pp. 321 - 325
Copyright
Copyright © Cambridge University Press 2011

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References

da Silvia Rascado, T., Martins, L.R., Minto, B.W., de Sa Lorena, S.E.R. & da Cruz Landim-Alvarenga, F. (2010). Parthenogenetic development of domestic cat oocytes treated with ionomycin, cycloheximide, roscovitine and strontium. Theriogenology 74, 596–601.CrossRefGoogle Scholar
De Azevedo, W.F., Leclerc, S., Meijer, L., Havlicek, L., Strnad, M. & Kim, S.H. (1997). Inhibition of cyclin-dependent kinases by purine analogues. Crystal structure of human cdk2 complexed with roscovitine. Eur. J. Biochem. 243, 518–26.CrossRefGoogle ScholarPubMed
Deng, M. & Shen, S.S. (2000). A specific inhibitor of p34 cdc2/cyclin B suppresses fertilization-induced calcium oscillations in mouse eggs. Biol. Reprod. 62, 873–8.CrossRefGoogle ScholarPubMed
Fulka, J. Jr., Leibfried-Rutledge, M.L. & First, N.L. (1991). Effect of 6-dimethylaminopurine on germinal vesicle breakdown of bovine oocytes. Mol. Reprod. Dev. 29, 379–84.CrossRefGoogle ScholarPubMed
Hyslop, L.A., Nixon, V.L., Levasseur, M., Chapman, F., Chiba, K., McDougall, A., Venables, J.P., Elliott, D.J. & Jones, K.T. (2004). Ca2+-promoted cyclin B1 degradation in mouse oocytes requires the establishment of a metaphase arrest. Dev. Biol. 269, 206–19.CrossRefGoogle Scholar
Jimenez-Macedo, A.R., Izquierdo, D., Urdaneta, A., Anguita, B. & Paramio, M.T. (2006). Effect of roscovitine on nuclear maturation, MPF and MAP kinase activity and embryo development of prepubertal goat oocytes. Theriogenology 65, 1769–82.CrossRefGoogle ScholarPubMed
Jones, K.T. (2004). Turning it on and off: M-phase promoting factor during meiotic maturation and fertilization. Mol. Hum. Reprod. 10, 1–5.CrossRefGoogle ScholarPubMed
Kubiak, J.Z. (1989). Mouse oocytes gradually develop the capacity for activation during the metaphase II arrest. Dev. Biol. 136, 537–45.CrossRefGoogle ScholarPubMed
Lu, Q., Zhao, Y., Gao, X., Li, Y., Ma, S., Mullen, S., Critser, J.K. & Chen, Z.J. (2006). Combination of calcium ionophore A23187 with puromycin salvages human unfertilized oocytes after ICSI. Eur. J. Obstet. Gynecol. Reprod. Biol. 126, 72–6.CrossRefGoogle ScholarPubMed
Meijer, L. & Kim, S.H. (1997). Chemical inhibitors of cyclin-dependent kinase. Methods Enzymol. 283, 113–28.CrossRefGoogle Scholar
Meijer, L., Borgne, A., Mulner, O., Chong, J.P.J., Blow, J.J., Inagaki, N., Inagaki, M., Delcros, J.G. & Moulinoux, J.P. (1997). Biochemical and cellular effects of roscovitine, a potent and selective inhibitor of the cyclin-dependent kinases cdc2, cdk2 and cdk5. Eur. J. Biochem. 243, 527–36.CrossRefGoogle ScholarPubMed
Meyerhof, P.G. & Masui, Y. (1977). Ca and Mg control of cytostatic factors from Rana pipiens oocytes which cause metaphase and cleavage arrest. Dev. Biol. 61, 214–29.CrossRefGoogle ScholarPubMed
Motlik, L., Pavlock, A., Kubelka, M., Kalous, J. & Kalab, P. (1998). Interplay between cdc2 kinase and MAP kinase pathway during maturation of mammalian oocytes. Theriogenology 49, 461–9.CrossRefGoogle ScholarPubMed
Murase, Y., Araki, Y., Mizuno, S., Kawaguchi, C., Naito, M., Yoshizawa, M. & Araki, Y. (2004). Pregnancy following chemical activation of oocytes in a couple with repeated failure of fertilization using ICSI: case report. Hum. Reprod. 19, 1604–7.CrossRefGoogle Scholar
Nagai, T. (1987). Parthenogenetic activation of cattle follicular oocytes in vitro maturation and with ethanol. Gamete Res. 16, 243–9.CrossRefGoogle ScholarPubMed
Nakagawa, K., Yamano, S., Moride, N., Yamashita, M., Yoshizawa, M. & Aono, T. (2001a). Effect of activation with Ca ionophore A23187 and puromycin on the development of human oocytes that failed to fertilize after intracytoplasmic sperm injection. Fertil. Steril. 76, 148–52.CrossRefGoogle ScholarPubMed
Nakagawa, K., Yamano, S., Nakasaka, H., Hinokio, K., Yoshizawa, M. & Aono, T. (2001b). A combination of calcium ionophore and puromycin to produce haploid parthenogenones with one haploid pronucleus. Zygote 9, 83–8.CrossRefGoogle ScholarPubMed
Nakasaka, H., Yamano, S., Hinokio, K., Nakagawa, K., Yoshizawa, M. & Aono, T. (2000). Effective activation method with A23187 and puromycin to produce haploid parthenogenones from freshly ovulated mouse oocytes. Zygote 8, 203–8.CrossRefGoogle ScholarPubMed
Powell, R. & Barnes, F.L. (1992). The kinetics of oocyte activation and polar body formation in bovine embryo clones. Mol. Reprod. Dev. 33, 53–8.CrossRefGoogle ScholarPubMed
Prochazka, R., Durnford, R., Fiser, P.S. & Marcus, G.J. (1993). Parthenogenetic development of activated in vitro matured bovine oocytes. Theriogenology 39, 1025–32.CrossRefGoogle ScholarPubMed
Sagata, N. (1996). Meiotic metaphase arrest in animal oocytes: its mechanisms and biological significance. Trends Cell Biol. 6, 22–8.CrossRefGoogle ScholarPubMed
Schoevers, E.J., Bevers, M.M., Roelen, B.A. & Colenbrander, B. (2005). Nuclear and cytoplasmic maturation of sow oocytes are not synchronized by specific meiotic inhibition with roscovitine during in vitro maturation. Theriogenology 63, 1111–30.CrossRefGoogle Scholar
Siracusa, G., Whittingham, D.G., Molinaro, M. & Vivarelli, E. (1978). Parthenogenetic activation of mouse oocytes induced by inhibitors of protein synthesis. J. Embryol. Exp. Morphol. 43, 157–66.Google ScholarPubMed
Sousa, M. & Tesarik, J. (1994). Ultrastructural analysis of fertilization failure after intracytoplasmic sperm injection. Hum. Reprod. 9, 2374–80.CrossRefGoogle ScholarPubMed
Tesarik, J. & Sousa, M. (1995). More than 90% fertilization rates after intracytoplasmic sperm injection and artificial induction of oocyte activation with calcium ionophore. Fertil. Steril. 63, 343–9.CrossRefGoogle ScholarPubMed
Wani, N.A. (2008). Chemical activation of in vitro matured dromedary camel (Camelus dromedaries) oocytes: optimization of protocols. Theriogenology 69, 591–602.CrossRefGoogle ScholarPubMed
Ware, C.B., Barnes, F.L., Maiki-Lauria, M. & First, N.L. (1989). Age dependence of bovine oocyte activation. Gamete Res. 22, 265–75.CrossRefGoogle ScholarPubMed
Yanagida, K., Katayose, H., Yazawa, H., Kimura, Y., Sato, A., Yanagimachi, H. & Yanagimachi, R. (1999). Successful fertilization and pregnancy following ICSI and electrical oocytes activation. Hum. Reprod. 14, 1307–11.CrossRefGoogle Scholar