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Role of follicle stimulating hormone and epidermal growth factor in the development of porcine preantral follicle in vitro

Published online by Cambridge University Press:  01 August 2007

Ji Wu  and
Qi Tian
Ji Wu*
Affiliation:
School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Qi Tian
Affiliation:
Temple University School of Medicine, Philadelphia, 19140, USA.
*
All correspondence to: Ji Wu, School of Life Science and Biotechnology, Shanghai Jiao Tong University, No. 800. Dongchuan Road, Minhang District, Shanghai, 200240, China. Tel: +86 21 34204933. Fax: +86 21 34204051. e-mail: [email protected]
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Summary

The aim of the present study was to assess the role of follicle stimulating hormone (FSH), epidermal growth factor (EGF) or a combination of EGF and FSH on the in vitro growth of porcine preantral follicles, estradiol secretion, antrum formation, oocyte maturation and subsequent embryonic development. Porcine preantral follicles were cultured for 3 days in the absence or in the presence of FSH or EGF. Oocytes from these follicles were then matured, fertilized in vitro and embryos were cultured. Estradiol secretion and histological analysis of cultured follicles were also carried out. The results showed that when FSH, or a combination of EGF and FSH, was added to the culture medium, most of preantral follicles grew to antral follicles with high estradiol secretion and the oocytes from these antral follicles could mature, fertilize and develop to the blastocyst stage. Without FSH, or a combination of EGF and FSH, preantral follicles were unable to develop to the antral stage. Histology demonstrated that the resulting follicles were nonantral, estradiol production was reduced and none of their oocytes matured after in vitro maturation. The results indicate the essential role of FSH in promoting in vitro growth of porcine preantral follicle, estradiol secretion, antrum formation, oocyte maturation and subsequent embryonic development. EGF with FSH treatment of porcine preantral follicles improves the quality of oocytes, shown by a higher frequency of embryonic development.

Keywords

EGFEmbryonic developmentFSHOocyte maturationPorcine preantral follicle
Type
Research Article
Information
Zygote , Volume 15 , Issue 3 , August 2007 , pp. 233 - 240
Copyright
Copyright © Cambridge University Press 2007

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