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Role of FGF signalling in neural crest cell migration during early chick embryo development

Published online by Cambridge University Press:  06 December 2018

Xiao-tan Zhang ,
Guang Wang ,
Yan Li ,
Manli Chuai ,
Kenneth Ka Ho Lee  and
Xuesong Yang Open the ORCID record for Xuesong Yang [Opens in a new window]
Xiao-tan Zhang
Affiliation:
Department of Histology and Embryology, International Joint Laboratory for Embryonic Development & Prenatal Medicine, Medical College, Jinan University, Guangzhou 510632, China Key Laboratory for Regenerative Medicine of the Ministry of Education, Jinan University, Guangzhou, China
Guang Wang
Affiliation:
Department of Histology and Embryology, International Joint Laboratory for Embryonic Development & Prenatal Medicine, Medical College, Jinan University, Guangzhou 510632, China Key Laboratory for Regenerative Medicine of the Ministry of Education, Jinan University, Guangzhou, China
Yan Li
Affiliation:
Department of Histology and Embryology, International Joint Laboratory for Embryonic Development & Prenatal Medicine, Medical College, Jinan University, Guangzhou 510632, China
Manli Chuai
Affiliation:
Division of Cell and Developmental Biology, University of Dundee, Dundee, DD1 5EH, UK
Kenneth Ka Ho Lee
Affiliation:
Joint CUHK-UoS Laboratory for Stem Cell and Regenerative Medicine, School of Biomedical Sciences, Chinese University of Hong Kong, Shatin, Hong Kong
Xuesong Yang*
Affiliation:
Department of Histology and Embryology, International Joint Laboratory for Embryonic Development & Prenatal Medicine, Medical College, Jinan University, Guangzhou 510632, China
*
Address for correspondence: Xuesong Yang. Department of Histology and Embryology, International Joint Laboratory for Embryonic Development & Prenatal Medicine, Medical College, Jinan University, Guangzhou 510632, China Fax: +20 85221343. E-mail: [email protected]
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Summary

Fibroblast growth factor (FGF) signalling acts as one of modulators that control neural crest cell (NCC) migration, but how this is achieved is still unclear. In this study, we investigated the effects of FGF signalling on NCC migration by blocking this process. Constructs that were capable of inducing Sprouty2 (Spry2) or dominant-negative FGFR1 (Dn-FGFR1) expression were transfected into the cells making up the neural tubes. Our results revealed that blocking FGF signalling at stage HH10 (neurulation stage) could enhance NCC migration at both the cranial and trunk levels in the developing embryos. It was established that FGF-mediated NCC migration was not due to altering the expression of N-cadherin in the neural tube. Instead, we determined that cyclin D1 was overexpressed in the cranial and trunk levels when Sprouty2 was upregulated in the dorsal neural tube. These results imply that the cell cycle was a target of FGF signalling through which it regulates NCC migration at the neurulation stage.

Keywords

Cell cycleEMTFGF signallingNeural crest cellsSprouty2
Type
Research Article
Information
Zygote , Volume 26 , Issue 6 , December 2018 , pp. 457 - 464
Copyright
© Cambridge University Press 2018 

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