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RNase A activity in Japanese quail oocytes

Published online by Cambridge University Press:  26 September 2008

Urszula Stepińska ,
Arleta Malewska  and
Bożenna Olszańska
Urszula Stepińska*
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiecn. Warsaw, 05-551 Mroków, Poland.
Arleta Malewska
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiecn. Warsaw, 05-551 Mroków, Poland.
Bożenna Olszańska
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiecn. Warsaw, 05-551 Mroków, Poland.
*
U. Stepińka, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-551 Mroków, Poland. Telephone: +48 (22) 7561 711. Fax: +48 (22) 7561 699.
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Summary

The presence of RNase A activity was studied in vitro in homogenates of quail oocytes and early embryos using [3H]poly(U) as a substrate. The activity was measured by adsorption of the undegraded substrate onto DE-81 filter paper discs and by chromatographic separation on a Sephadex G-50 column. RNase A activity examined by these methods was almost undetectable in quail previtellogenic, vitellogenic and ovulated oocytes as well as in the embryos from laid eggs. It is estimated to be about 1.1 × 10−5 Kunitz units per ovulated oocyte. Higher activity starts to appear in gastrulating embryos. These findings are discussed in relation to other studies demonstrating the high stability of maternal RNA during early development, especially in growing oocytes.

Keywords

Maternal RNAOocyteQuailRNA degradationRNase A activity
Type
Article
Information
Zygote , Volume 4 , Issue 3 , August 1996 , pp. 219 - 227
Copyright
Copyright © Cambridge University Press 1996

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References

Bachvarova, R.. (1981). Synthesis, turnover and stability of heterogeneous RNA in growing mouse oocytes. Dev. Biol. 86, 384–92.CrossRefGoogle ScholarPubMed
Bernstein, P., Peltz, S.W. & Ross, J.. (1989). The poly(A)– poly(A)-binding protein complex is a major determinant of mRNA stability in vitro. Mol. Cell Biol 9, 659–70.Google Scholar
Bouvet, P., Paris, J., Philippe, M. & Osborne, H.B.. (1991). Degradation of a developmentally regulated mRNA in Xenopus embryos is controlled by the 3' region and requires the translation of another maternal mRNA. Mel. Cell Biol 11, 3115–24.Google Scholar
Brower, P.T., Gizang, E., Boreen, S.M. & Schultz, R.M.. (1981). Biochemical studies of mammalian oogenesis: synthesis and stability of various classes of RNA during growth of the mouse oocyte in vitro. Dev. Biol 86, 373–83.Google Scholar
Callebaut, M.. (1968). [3H]Uridine incorporation during previtellogenesis and early vitellogenesis in oocytes of the chick (Gallus gallus). J. Embryol. Exp. Morphol 20, 169–74.Google Scholar
Darnbrough, C.H. & Ford, P.J.. (1981). Identification in Xenopus laevis of a class of oocyte-specific proteins bound to messenger RNA. Eur. J. Biochem 113, 415–24.CrossRefGoogle ScholarPubMed
Delany, M.E.. Taylor, R.L. Jr & Bloom, S.E.. (1995). Teratogenic development in chicken embryos associated with a major deletion in the rRNA gene cluster. Dev. Growth Differ 37, 403–12.Google Scholar
Duval, C., Bouvet, P., Omilli, F., Roghi, C., Dorel, C., LeGuellec, R., Paris, J. & Osborne, H.B.. (1990). Stability of maternal mRNA in Xenopus embryos: role of transcription and translation. Mol. Cell Biol 10, 4123–9.Google ScholarPubMed
Ebert, K.M., Paynton, B.V., McKnight, G.S. & Brinster, R.L.. (1984). Translation and stability of ovalbumin messenger RNA injected into growing oocytes and fertilized ova of mice. J. Embryol. Exp. Morphol 84, 91–103.Google ScholarPubMed
Eyal-Giladi, H & Kochav, S.. (1976). From cleavage to primitive streak formation: a complementary normal table and new look at the first stages of the development of the chick. I. General morphology. Dev. Biol 49, 321–37.Google Scholar
Ford, P.J., Mathieson, T. & Rosbash, M.. (1977). Very long-lived messenger RNA in ovaries of Xenopus laevis. Dev. Biol 57, 417–26.CrossRefGoogle ScholarPubMed
Gurdon, J.B., Lingrel, J.B. & Marbaix, G.. (1973). Message stability in injected frog oocytes: long life of mammalian α and βglobin messages. J. Mol. Biol 80. 539–51.CrossRefGoogle Scholar
Hamburger, V. & Hamilton, H.L.. (1951). A series of normal stages in the development of the chick embryo. J. Morphol 88, 49–92.CrossRefGoogle ScholarPubMed
Jackson, R.J. & Standart, N.. (1990). Do the poly(A) tail and 3' untranslated region control mRNA translation? Cell 62, 15–24.CrossRefGoogle ScholarPubMed
Jahn, C.L., Baran, M.M. & Bachvarova, R.. (1976). Stability of RNA synthesized by the mouse oocyte during its major growth phase. J. Exp. Zool 197, 161–72.Google Scholar
Liao, Y.D.. (1992). A pyrimidine–guanine sequence-specific ribonuclease from Rana catesbeiana (bullfrog) oocytes. Nucleic Acids Res. 20, 1371–7.Google Scholar
Lodish, H.F. & Small, B.. (1976). Different life-times of reticulocyte messenger RNA. Cell 7, 59–65.Google Scholar
Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J.. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–75.Google Scholar
Maniatis, T., Fritsch, E.F. & Sambrook, J.. (1982). Molecular Cloning: A Laboratory Manual. New York: Cold Spring Hathor Laboratory Press.Google Scholar
Mohun, T.J., Garrett, N. & Taylor, M.V.. (1989). Temporal and tissue-specific expression of proto-oncogene c-fos during development in Xenopus laevis. Development 107, 835–46.Google Scholar
Müller, W.E.G.. (1976). Endoribonuclease IV. A poly(A)specific ribonuclease from chick oviduct. 1. Purification of the enzyme. Eur. J. Biochem 70, 241–8.CrossRefGoogle ScholarPubMed
Olszańska, B. & Borgul, A.. (1993). Maternal RNA content in oocytes of several mammalian and avian species. J. Exp. Zool 265, 317–20.CrossRefGoogle ScholarPubMed
Olszańska, B., Kludkiewicz, B. & Lassota, Z.. (1984). Transcription and polyadenylation processes during early development of quail embryo. J. Embryol. Exp. Morphol 79, 11–24.Google ScholarPubMed
Paynton, B.V., Rempel, R. & Bachvarova, R.. (1988). Changes in state of adenylation and time course of degradation of maternal mRNAs during oocyte maturation and early embryonic development in the mouse. Dev. Biol 129, 304–14.CrossRefGoogle ScholarPubMed
Piko, L. & Clegg, K.B.. (1982). Quantitative changes in total RNA, total poly(A), and ribosomes in early mouse embryos. Dev. Biol 89, 362–78.CrossRefGoogle ScholarPubMed
Raveh, D.. Friedlander, M. & Eyal-Giladi, H. (1976). Nucleolar ontogenesis in the uterine chick germ correlated with morphological events. Exp. Cell Res. 100, 195–203.Google Scholar
Ross, J.. (1988). Messenger RNA turnover in eukaryotic cells. Mol. Biol. Med. 5, 1–14.Google ScholarPubMed
Sagata, N., Shiokawa, K. & Yamana, K.. (1980). A study on the steady state population of poly(A) +RNA during early development of Xenopus laevis. Dev. Biol 77,431–48.Google Scholar
Shaw, C. & Kamen, R.. (1986). A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation. Cell 46, 659–67.CrossRefGoogle ScholarPubMed
Spirin, A.S.. (1994). Storage of messenger RNA in eukaryotes: envelopment with protein, translational barrier at 5' side or conformational masking by 3' side?. Mol Reprod. Dev. 38, 107–17.Google Scholar
Taylor, M.V., Gusse, M., Evan, G.I., Dathan, N. & Mechali, M.. (1986). Xenopus myc proto-oncogene during development: expression as a stable maternal mRNA uncoupled from cell division. EMBO J. 5, 3563–70.Google Scholar
Treisman, R.. (1985). Transient accumulation of c-fos RNA following serum stimulation requires a conserved 5' element and c-fos 3' sequences. Cell 42, 889–902.Google Scholar
Wang, J.J., Tang, P.C., Chao, S.H., Cheng, C.H., Ma, H.J. & Liao, Y.D.. (1995). Immunocytochemical localization of ribonuclease in yolk granules of adult Rana catesbeiana oocytes. Cell Tissue Res. 280, 259–65.Google Scholar
Wylie, C.C.. (1972). The appearance and quantitation of cytoplasmic ribonucleic acid in the early chick embryo. J. Embryol. Exp. Morphol 28, 367–84.Google Scholar
Xing, Y.Y. & Worcel, A.. (1989). A 3' exonuclease activity degrades the pseudogene 5S RNA transcript and processes the major oocyte 5S RNA transcript in Xenopus oocytes. Genes Dev. 3, 1008–18.Google Scholar