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Regulated expression of TAF1 in 1-cell mouse embryos

Published online by Cambridge University Press:  01 August 2006

Kai Wang
Affiliation:
Center for Developmental Biology, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, P. R. China.
Feng Sun
Affiliation:
Laboratory of Stem Cell Biology, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, P. R. China.
Hui Z. Sheng*
Affiliation:
Laboratory of Stem Cell Biology, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, P. R. China. Center for Developmental Biology, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, P. R. China.
*
All correspondence to: Hui Z. Sheng, MD, PhD, Center for Developmental Biology, 1665 Kong-Jiang Road, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200092, P. R. China. Tel: +86 21 55570361. Fax: +86 21 55570017. e-mail: [email protected]

Summary

TATA binding protein (TBP) associated factor 1 (TAF1) is a member of the general transcription machinery. Interference in the function of TAF1 causes a broad transcriptional defect in early development. To explore possible roles of TAF1 in embryonic transcriptional silence and zygotic genome activation, we examined the expression of TAF1 in 1-cell mouse embryos. Using an immunofluorescence assay, TAF1 was not detected in embryos in the first few hours after fertilization. TAF1 appeared in pronuclei 6 h post-fertilization and reached a relatively high level before zygotic genome activation. These data show that besides TBP, another critical member of the general transcription machinery such as TAF1 is also absent or at an extremely low level at the outset of development. Combined deficiency in critical members of the general transcription machinery may account for embryonic transcriptional silence.

Keywords

Type
Research Article
Copyright
Copyright © Cambridge University Press 2006

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