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Protein synthesis is not required for male pronuclear formation in bovine zygotes

Published online by Cambridge University Press:  26 September 2008

R.C. Chian  and
M.A. Sirard
R.C. Chian
Affiliation:
Centre de Recherche en Biologie de la Reproduction, Département des sciences animales, Université Laval, Québec, Canada.
M.A. Sirard*
Affiliation:
Centre de Recherche en Biologie de la Reproduction, Département des sciences animales, Université Laval, Québec, Canada.
*
Dr M.A. Sirard, Département des sciences animales, Université Laval, Québec, PQ, Canada GIK 7P4. Fax: +1(418)-656-3766.
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Summary

Following fertilisation, the sperm triggers a series of intracellular changes which initiate oocyte activation and pronuclear formation. Oocyte activation can also be induced artificially by several chemicals, such as the calcium ionophore A23187. The sperm nucleus is transformed into the male pronucleus through the interaction of oocyte cytoplasmic factors. The profile of protein synthesis is different in bovine oocytes following fertilisation and parthenogenetic activation. The formation of male and female pronuclei was not blocked by the presence of the protein synthesis inhibitor cycloheximide. These results suggest that bovine oocyte activation by sperm and parthenogenetic activation induce different cytoplasmic responses for protein synthesis and that new protein synthesis is not required for male pronuclear formation in bovine zygotes.

Keywords

CycloheximideFertilisationIonophore A23187Oocyte activationProtein synthesis
Type
Article
Information
Zygote , Volume 4 , Issue 1 , February 1996 , pp. 41 - 48
Copyright
Copyright © Cambridge University Press 1996

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