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Pronuclear formation and intracellular glutathione content of in vitro-matured porcine oocytes following in vitro fertilisation and/or electrical activation

Published online by Cambridge University Press:  26 September 2008

Hiroaki Funahashi ,
Todd T. Stumpf ,
Thomas C. Cantley ,
Nam-Hyung Kim  and
Billy N. Day
Hiroaki Funahashi
Affiliation:
Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri USA
Todd T. Stumpf
Affiliation:
Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri USA
Thomas C. Cantley
Affiliation:
Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri USA
Nam-Hyung Kim
Affiliation:
Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri USA
Billy N. Day*
Affiliation:
Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri USA
*
Billy N. Day, University of Missouri Columbia, Department of Animal Sciences, 159 Animal Sciences Center, Columbia, MO 65211, USA. Telephone: (314) 882-7555. Fax: (314) 882-6827.
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Summary

Pronuclear formation and intracellular content of glutathione, containing reduced and oxidised forms, in porcine oocytes matured in vitro were determined following insemination and/or electrical stimulation. After insemination, sperm penetration had occurred as early as 3 h and female pronuclei had formed by 6 h with complete development by 12 h. Male pronuclear formation occurred, primarily, between 9 and 12 h after insemination. Glutathione content of the oocytes decreased following sperm penetration and remained at a depressed level until 12 h. After electrical stimulation, oocyte activation had occurred and female pronuclei had formed by 3 and 6 h, respectively. Oocyte glutathione content did not change as a result of oocyte activation. When oocytes were exposed to an electrical pulse and then spermatozoa, female pronuclear formation was observed by 3 h after stimulation/insemination. Sperm penetration was observed between 3 and 9 h. However, the incidence of male pronuclear formation observed at 12 h was extremely low, although sperm decondensation had occurred in some oocytes. Oocyte glutathione content had not decreased by 6 h following electrical activation. These results demonstrate that the changes in glutathione content in porcine oocytes following fertilisation in vitro differ from those due to electrical activation. Further, the decreased intracellular glutathione content in oocytes activated by sperm penetration appears to be due to the presence of a sperm factor.

Keywords

FertilisationGlutathioneOocytePigPronuclear formation
Type
Article
Information
Zygote , Volume 3 , Issue 3 , August 1995 , pp. 273 - 281
Copyright
Copyright © Cambridge University Press 1995

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