Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-22T15:11:28.970Z Has data issue: false hasContentIssue false
  • English
  • Français

Prolonged interval time between blastocyst biopsy and vitrification compromised the outcomes in preimplantation genetic testing

Published online by Cambridge University Press:  18 February 2021

Shun Xiong Open the ORCID record for Shun Xiong [Opens in a new window] ,
Jun Xia Liu ,
Dong Yun Liu ,
Jia Hong Zhu ,
Xiang Wei Hao ,
Li Hong Wu ,
Yang Gao ,
Jing Yu Li  and
Guo Ning Huang Open the ORCID record for Guo Ning Huang [Opens in a new window]
Shun Xiong
Affiliation:
Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China
Jun Xia Liu
Affiliation:
Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China
Dong Yun Liu
Affiliation:
Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China
Jia Hong Zhu
Affiliation:
Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China
Xiang Wei Hao
Affiliation:
Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China
Li Hong Wu
Affiliation:
Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China
Yang Gao
Affiliation:
Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China
Jing Yu Li
Affiliation:
Chongqing Key Laboratory of Human Embryo Engineering, Chongqing, People’s Republic of China
Guo Ning Huang*
Affiliation:
Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China
*
Author for correspondence: Guo Ning Huang. Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

This study aimed to evaluate to what extent the different interval times between trophectoderm (TE) biopsy and vitrification influence the clinical outcomes in preimplantation genetic testing (PGT) cycles. Patients who underwent frozen embryo transfer (FET) after PGT between 2015 and 2019 were recruited. In total, 297 cycles with single day 5 euploid blastocyst transfer were included. These cycles were divided into three groups according to the interval times: <1 h group, 1–2 h group, and ≥2 h group. Blastocyst survival, clinical pregnancy, miscarriage, and ongoing pregnancy rates were compared. The results showed that, in PGT-SR cycles, survival rate in the ≥2 h group (96.72%) was significantly lower than in the <1 h group (100%, P = 0.047). The clinical pregnancy rate in the ≥2 h group was 55.93%, significantly lower than in the <1 h group (74.26%, P = 0.017). The ongoing pregnancy rates in the 1–2 h group and the ≥2 h group were 48.28% and 47.46%, respectively, significantly lower than that in the <1 h group (67.33%, P < 0.05). The miscarriage rate in the 1–2 h group was 18.42%, significantly higher than that in the <1 h group (5.33%, P = 0.027). In PGT-A cycles, the clinical pregnancy and ongoing pregnancy rates in the <1 h group were 67.44% and 53.49%, respectively, higher than that in the 1–2 h group (52.94%, 47.06%, P > 0.05) and the ≥2 h group (52.63%, 36.84%, P > 0.05). In conclusion, vitrification of blastocysts beyond 1 h after biopsy significantly influences embryo survival and clinical outcomes and is therefore not recommended.

Keywords

Blastocyst biopsyClinical pregnancy rateInterval timeOngoing pregnancy rateVitrification
Type
Research Article
Information
Zygote , Volume 29 , Issue 4 , August 2021 , pp. 276 - 281
Copyright
© The Author(s), 2021. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

These authors contributed equally to the research and preparation of the manuscript.

References

Chen, HH, Huang, CC, Cheng, EH, Lee, TH, Chien, LF and Lee, MS (2017). Optimal timing of blastocyst vitrification after trophectoderm biopsy for preimplantation genetic screening. PLoS One 12, e0185747.CrossRefGoogle ScholarPubMed
Cimadomo, D, Capalbo, A, Levi-Setti, PE, Soscia, D, Orlando, G, Albani, E, Parini, V, Stoppa, M, Dovere, L, Tacconi, L, Ievoli, E, Maggiulli, R, Ubaldi, FM and Rienzi, L (2018). Associations of blastocyst features, trophectoderm biopsy and other laboratory practice with post-warming behavior and implantation. Hum Reprod 11, 1992–2001.CrossRefGoogle Scholar
Darwish, E and Magdi, Y (2016). Artificial shrinkage of blastocoel using a laser pulse prior to vitrification improves clinical outcome. J Assist Reprod Genet 33, 467–71.CrossRefGoogle ScholarPubMed
Desai, N, Szeptycki, J, Scott, M, AbdelHafez, FF and Goldfarb, J (2008). Artificial collapse of blastocysts before vitrification: mechanical vs. laser technique and effect on survival, cell number, and cell death in early and expanded blastocysts. Cell Preserv Technol 6, 181–90.CrossRefGoogle Scholar
ESHRE PGT Consortium and SIG-Embryology Biopsy Working Group, Kokkali, G, Coticchio, G, Bronet, F, Celebi, C, Cimadomo, D, Goossens, V, Liss, J, Nunes, S, Sfontouris, I, Vermeulen, N, Zakharova, E, De Rycke, M. ESHRE PGT Consortium and SIG Embryology good practice recommendations for polar body and embryo biopsy for PGT (2020). Hum Reprod Open 2020, hoaa020. doi: 10.1093/hropen/hoaa020.CrossRefGoogle Scholar
Guzman, L, Nunez, D, Lopez, R, Inoue, N, Portella, J, Vizcarra, F, Noriega-Portella, L, Noriega-Hoces, L and Munne, S (2019). The number of biopsied trophectoderm cells may affect pregnancy outcomes. J Assist Reprod Genet 36, 145–51.CrossRefGoogle ScholarPubMed
Handyside, AH, Kontogianni, EH, Hardy, K and Winston, RM (1990). Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification. Nature 19, 768–70.CrossRefGoogle Scholar
Hiraoka, K, Hiraoka, K, Kinutani, M and Kinutani, K (2004). Blastocoele collapse by micropipetting prior to vitrification gives excellent survival and pregnancy outcomes for human day 5 and 6 expanded blastocysts. Hum Reprod 19, 2884–88.CrossRefGoogle ScholarPubMed
Homer, H (2019). Preimplantation genetic testing for aneuploidy (PGT-A): the biology, the technology and the clinical outcomes. Aust N Z J Obstet Gynaecol 59, 317–24.CrossRefGoogle ScholarPubMed
Levi-Setti, PE, Menduni, F, Smeraldi, A, Patrizio, P, Morenghi, E and Albani, E (2016). Artificial shrinkage of blastocysts prior to vitrification improves pregnancy outcome: analysis of 1028 consecutive warming cycles. J Assist Reprod Genet 33, 461–66.CrossRefGoogle ScholarPubMed
Maggiulli, R, Giancani, A, Cimadomo, D, Ubaldi, FM and Rienzi, L (2019). Human blastocyst biopsy and vitrification. J Vis Exp 149, doi: 10.3791/59625. PMID: 31403619.CrossRefGoogle Scholar
Pooyanfar, F, Foroutan, T and Dashtizad, M (2018). Effects of blastocyst artificial collapse prior to vitrification on hatching and survival rates and the expression of klf4 gene in mouse embryos. Vet Res Forum 9, 87–92.Google ScholarPubMed
Scott, KL, Hong, KH and Scott, RT (2013). Selecting the optimal time to perform biopsy for preimplantation genetic testing. Fertil Steril 100, 608–14.CrossRefGoogle ScholarPubMed
Scott, RT, Upham, KM, Forman, EJ, Zhao, T and Treff, NR (2013). Cleavage-stage biopsy significantly impairs human embryonic implantation potential while blastocyst biopsy does not: a randomized and paired clinical trial. Fertil Steril 100, 624–30.CrossRefGoogle Scholar
Vanderzwalmen, PB, Debauche, Ch and Schoysman, R (2002). Births after vitrification at morula and blastocyst stages: effect of artificial reduction of the blastocoelic cavity before vitrification. J Assist Reprod Genet 33, 461–66.Google Scholar
Wang, C, Feng, G, Zhang, B, Zhou, H, Shu, J, Lin, R, Chen, H and Wu, Z (2017). Effect of different artificial shrinkage methods, when applied before blastocyst vitrification, on perinatal outcomes. Reprod Biol Endocrinol 15, 32.CrossRefGoogle ScholarPubMed
Xiong, S, Han, W, Liu, JX, Zhang, XD, Liu, WW, Liu, H and Huang, GN (2011). Effects of cumulus cells removal after 6 h co-incubation of gametes on the outcomes of human IVF. J Assist Reprod Genet 28, 1205–11.CrossRefGoogle ScholarPubMed
Xiong, S, Liu, JX, Gao, Y, Liu, WW, Wu, LH, Han, W, Zhang, XD, Han, SB, Liu, DY and Huang, GN (2016). Shortened equilibration time can compromise clinical outcomes in human embryo vitrification. Hum Fertil (Camb) 19, 114–19.CrossRefGoogle ScholarPubMed
Ye, H, Huang, GN, Zeng, PH and Pei, L (2009). IVF/ICSI outcomes between cycles with luteal estradiol (E2) pre-treatment before GnRH antagonist protocol and standard long GnRH agonist protocol: a prospective and randomized study. J Assist Reprod Genet 26, 105–11.CrossRefGoogle ScholarPubMed
Zhao, H, Tao, W, Li, M, Liu, H, Wu, K and Ma, S (2019). Comparison of two protocols of blastocyst biopsy submitted to preimplantation genetic testing for aneuploidies: a randomized controlled trial. Arch Gynecol Obstet 299, 1487–93.CrossRefGoogle ScholarPubMed