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Production of buffalo embryos using oocytes from in vitro grown preantral follicles

Published online by Cambridge University Press:  01 February 2008

P.S.P. Gupta ,
H.S. Ramesh ,
B.M. Manjunatha ,
S. Nandi  and
J.P. Ravindra
P.S.P. Gupta*
Affiliation:
National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore-560 030, India.
H.S. Ramesh
Affiliation:
National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore-560 030, India.
B.M. Manjunatha
Affiliation:
National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore-560 030, India.
J.P. Ravindra
Affiliation:
National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore-560 030, India.
*
1All correspondence to: P.S.P. Gupta, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore-560 030, India. E-mail: [email protected]; [email protected]
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Summary

The present study examines the use of buffalo preantral follicles as a source of oocytes for in vitro embryo production. Preantral follicles were isolated from abattoir-derived buffalo ovaries and were grown for 100 days in five different culture systems: (1) minimum essential medium (MEM); (2) coconut water; (3) MEM + ovarian mesenchymal cell (OMC) co-culture; (4) MEM + granulosa cell (GC) co-culture; or (5) MEM + cumulus cell (CC) co-culture. Low growth rates for the preantral follicles were observed when follicles were cultured in MEM or coconut water medium. Moderate growth rates were seen for OMC and GC co-cultures, and high rates of growth were observed when follicles were grown in CC co-culture. The survival of preantral follicles was low in the MEM culture (<25%), but was over 75% in the other culture systems. Oocytes were not recovered from the MEM group, while an oocyte recovery rate of 80–100% was observed when the follicles were cultured with coconut water/somatic cells. Transferable embryos could be produced only with the oocytes obtained from preantral follicles grown in the OMC and CC co-culture systems. This study demonstrates, for the first time, that it is possible to produce buffalo embryos by in vitro fertilization of oocytes derived from in vitro grown preantral follicles.

Keywords

BuffaloEmbryoIn vitro culturePreantral follicleSomatic cells
Type
Research Article
Information
Zygote , Volume 16 , Issue 1 , February 2008 , pp. 57 - 63
Copyright
Copyright © Cambridge University Press 2008

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