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Potential role of intraspecific and interspecific cloning in the conservation of wild mammals

Published online by Cambridge University Press:  11 June 2019

Alana Azevedo Borges  and
Alexsandra Fernandes Pereira Open the ORCID record for Alexsandra Fernandes Pereira [Opens in a new window]
Alana Azevedo Borges
Affiliation:
Laboratory of Animal Biotechnology, Federal Rural University of Semi-Arid, Mossoró, RN, Brazil
Alexsandra Fernandes Pereira*
Affiliation:
Laboratory of Animal Biotechnology, Federal Rural University of Semi-Arid, Mossoró, RN, Brazil
*
*Address for correspondence: Alexsandra Fernandes Pereira. Laboratory of Animal Biotechnology, Federal Rural University of Semi-Arid, Av. Francisco Mota, 572, Mossoró, RN, 59625-900, Brazil. Tel: +55 84 3317 8361. E-mail address: [email protected]
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Summary

Intraspecific and interspecific cloning via somatic cell nuclear transfer (iSCNT) is a biotechnique with great possibilities for wild mammals because it allows the maintenance of biodiversity by recovering species, nuclear reprogramming for the production of pluripotency-induced cells, and studies related to embryonic development. Nevertheless, many areas in cloning, especially those associated with wild mammals, are still in question because of the difficulty in obtaining cytoplasmic donor cells (or cytoplasts). Conversely, donor cell nuclei (or karyoplasts) are widely obtained from the skin of living or post-mortem individuals and often maintained in somatic cell banks. Moreover, the creation of karyoplast–cytoplast complexes by fusion followed by activation and embryo development is one of the most difficult steps that requires further clarification to avoid genetic failures. Although difficult, cloning different species, such as wild carnivores and ungulates, can be successful via iSCNT with embryo development and the birth of offspring. Thus, novel research in the area that contributes to the conservation of biodiversity and knowledge of the physiology of species continues. The present review presents the failures and successes that occurred with the application of the technique in wild mammals, with the goal of helping future work on cloning via iSCNT.

Keywords

BiodiversityBiotechnologyEmbryo developmentSCNTSomatic cells
Type
Review Article
Information
Zygote , Volume 27 , Issue 3 , June 2019 , pp. 111 - 117
Copyright
© Cambridge University Press 2019 

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